--------------------------- *** General information *** --------------------------- These datasets contain data from: Konrad M, Vyleta ML, Theis FJ, Tragust S, Stock M, Klatt M, Drescher V, Marr C, Ugelvig LV & Cremer S (2012) Social Transfer of Pathogenic Fungus Promotes Active Immunisation in Ant Colonies. PLoS Biology 10(4): e1001300. doi:10.1371/journal.pbio.1001300 Data collectors: Matthias Konrad, Meghan Vyleta, Miriam Stock, Line Vej Ugelvig Email addresses: Matthias.Konrad@ist.ac.at, Meghan.Vyleta@ist.ac.at, Miriam.Stock@ist.ac.at, Line.Ugelvig@ist.ac.at Datasets are saved as CSV files. There are no special symbols for missing data. Missing data are simply empty cells in the spreadsheed. ----------------- *** File list *** ----------------- Konrad_etal_2012_Figure 1.csv Konrad_etal_2012_Figure 2.csv Konrad_etal_2012_Figure 3.csv Konrad_etal_2012_Figure 4.csv Konrad_etal_2012_Figure 5AB.csv Konrad_etal_2012_Figure 5CD.csv Konrad_etal_2012_Figure 6.csv Konrad_etal_2012_Figure 7.csv Konrad_etal_2012_Figure S4.csv Konrad_etal_2012_Spatial Separation experiment.csv -------------------------------- *** Description of the files *** -------------------------------- Konrad_etal_2012_Figure 1.csv This file contains the data which was used to analyse the antifungal activity of nestmates after social contact to a treated individual Data collector: Matthias Konrad Columns: - "Day": Length of social contact between nestmates and the treated individual (coded as: 1 = 3days, 2 = 5days) - "Treatment": Treatment of the directly treated individual (coded as: 1 = Control treated, 2 = Fungus exposed) - "Absorbance": Absorbance values of antifungal activity assay samples (medium + fungal blastospores + ant sample) after 24h of growth measured with a spectrophotometer - "Absorbance values growth control": Absorbance values of growth control samples (medium + fungal blastospores) after 24h of growth measured with a spectrophotometer ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Konrad_etal_2012_Figure 2.csv This file contains the data which was used to analyse the behavioural interactions among group members Data collector: Line Vej Ugelvig Columns: - "Pairing": Type of ants participating in an interaction (coded as: 1 = directly treated ant interacting with a nestmate, 2 = nestmates interacting with each other) - "Treatment": Treatment of the directly treated individual (coded as: 2 = Control treated, 4 = Fungus exposed) - "Population": Four different populations were used in the experiment (coded as: 1 = Jena-Germany, 2 = Volterra-Spain, 3 = Bellaterra-Spain, 4 = Seva-Spain) - "Replica": Arbitrary labelling of experimental groups (ants from the same experimental group have the same labelling) - "From Individual": Identity of the ant performing the behaviour (coded from 1-6, with: 1 = directly treated ants, 2-6 = nestmates) - "To Individual": Identity of the ant receiving the behaviour (coded from 1-6, with: 1 = directly treated ants, 2-6 = nestmates) - "Day": Timepoint of observation (in days post treatment of the directly treated individual) - "Trophallaxis": cumulative frequency of trophallaxis (exchange of regurgitated liquid food) performed by the individual throughout the experiment - "Allogrooming": cumulative frequency of allogrooming (mutual cleaning of the body surface) performed by the individual throughout the experiment - "Antennation": cumulative frequency of antennation (recognition behaviour) performed by the individual throughout the experiment ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Konrad_etal_2012_Figure 3.csv This file contains the data which was used to analyse the fungal infection levels of fungus exposed individuals and their nestmates Data collector: Matthias Konrad Columns: - "Type": Type of ant (coded as: 1 = directly fungus exposed ant, 2 = nestmate of a directly fungus exposed ant) - "Day": Timepoint of dissection (coded as: 1 = 1day post exposure, 2 = 3days post exposure, 3 = 5days post exposure) - "Status": Status of an ant before freezing (coded as: 1 = alive, 2 = dead) - "Presence of CFUs": Presence of 1 or more colony forming units on an agar plate (coded as: 1 = yes, 2 = no) - "Number of CFUs": Total number of colony forming units on an agar plate - "Group ID": Arbitrary labelling of experimental groups (nestmates from the same experimental group have the same labelling) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Konrad_etal_2012_Figure 4.csv This file contains the data which was used to analyse the antifungal activity of directly exposed individuals Data collector: Matthias Konrad Columns: - "Treatment": Treatment of the directly treated individual (coded as: 1 = Control treated, 2 = exposed to a low dose of the fungus, 3 = exposed to a high dose of the fungus) - "Absorbance": Absorbance values of antifungal activity assay samples (medium + fungal blastospores + ant sample) after 24h of growth measured with a spectrophotometer - "Absorbance values growth control": Absorbance values of growth control samples (medium + fungal blastospores) after 24h of growth measured with a spectrophotometer ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Konrad_etal_2012_Figure 5AB.csv This file contains the data which was used to analyse the antifungal activity of 'new nestmates' Data collectors: Matthias Konrad, Line Vej Ugelvig Columns: - "Type": Type of ant (coded as: 1 = new nestmates of a treated individual, 2 = new nestmates of early nestmates) - "Treatment": Treatment of the directly treated individual (coded as: 1 = Control treated, 2 = Fungus exposed) - "Absorbance": Absorbance values of antifungal activity assay samples (medium + fungal blastospores + ant sample) after 24h of growth measured with a spectrophotometer - "Absorbance values growth control": Absorbance values of growth control samples (medium + fungal blastospores) after 24h of growth measured with a spectrophotometer ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Konrad_etal_2012_Figure 5CD.csv This file contains the data which was used to analyse the antifungal activity of different ant body parts and exudates Data collector: Matthias Konrad Columns: - "Type": Type of ant (coded as: 1 = directly treated ant, 2 = nestmate of a directly treated ant) - "Sample": Type of ant material tested in the antifungal activity assay (coded as: 1 = cuticle, 2 = thorax, 3 = trophallactic droplet) - "Treatment": Treatment of the directly treated individual (coded as: 1 = Control treated, 2 = Fungus exposed) - "Absorbance": Absorbance values of antifungal activity assay samples (medium + fungal blastospores + ant sample) after 24h of growth measured with a spectrophotometer ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Konrad_etal_2012_Figure 6.csv This file contains the data which was used to analyse the immune gene expression of nestmates after social contact to a treated individual Data collector: Meghan Vyleta Columns: - "Treatment": Treatment of the directly treated individual (coded as: 1 = Control treated, 2 = Fungus exposed) - "Defensin": Expression (in ng) of immune gene, Defensin, as measured by qPCR - "PPO": Expression (in ng) of immune gene, PPO, as measured by qPCR - "Cathepsin": Expression (in ng) of immune gene, Cathepsin L, as measured by qPCR - "18S": Expression (in ng) of housekeeping gene, 18s rRNA, as measured by qPCR - "Defensin/18S": Expression of immune gene, Defensin, normalised to housekeeping gene 18S rRNA - "PPO/18S": Expression of immune gene, PPO, normalised to housekeeping gene 18S rRNA - "Cathepsin/18S": Expression of immune gene, Cathepsin L, normalised to housekeeping gene 18S rRNA ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Konrad_etal_2012_Figure 7.csv This file contains the data which was used to analyse the antibacterial activity of nestmates after social contact to a treated individual Data collector: Miriam Stock Columns: - "Treatment": Treatment of the directly treated individual (coded as: 1 = Control treated, 2 = Fungus exposed) - "Absorbance": Absorbance values of antibacterial activity assay samples (medium + bacteria + ant sample) after 3h of growth measured with a spectrophotometer - "Absorbance values growth control": Absorbance values of growth control samples (medium + bacteria) after 3h of growth measured with a spectrophotometer ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Konrad_etal_2012_Figure S4.csv This file contains the data which was used to analyse the immune gene expression in ants after bacterial septic injury Data collectors: Meghan Vyleta Columns: - "Gene": Gene tested (coded as: 1 = Defensin, 2 = PPO, 3 = Cathepsin L) - "Treatment": Treatment of the directly treated individual (coded as: 1 = control pricked, 2 = pricked with bacteria) - "Expression normalised to 18S": Expression of genes of interest (Defensin, PPO, and Cathepsin L) normalized to expression of 18S rRNA housekeeping gene - "18S": Expression (in ng) of 18S rRNA housekeeping gene as measured by qPCR - "Defensin": Expression (in ng) of Defensin immune gene as measured by qPCR - "PPO": Expression (in ng) of PPO immune gene as measured by qPCR - "Cathepsin L": Expression (in ng) of Cathepsin L immune gene as measured by qPCR ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Konrad_etal_2012_Spatial Separation experiment.csv This file contains the data which was used to analyse the antifungal activity of nestmates spatially separated from the treated individual Data collectors: Line Vej Ugelvig, Matthias Konrad Columns: - "Treatment": Treatment of the directly treated individual (coded as: 1 = Control treated, 2 = Fungus exposed) - "Absorbance": Absorbance values of antifungal activity assay samples (medium + fungal blastospores + ant sample) after 24h of growth measured with a spectrophotometer - "Absorbance values growth control": Absorbance values of growth control samples (medium + fungal blastospores) after 24h of growth measured with a spectrophotometer