# Readme file of the dataset for the playback experiment of the manuscript “EVOLUTION OF DIVERGENT FEMALE MATING PREFERENCE IN RESPONSE TO EXPERIMENTAL SEXUAL SELECTION” Debelle A, Ritchie MG & Snook RR 2014, Evolution. The dataset file is called “Female_preference_dataset_Dryad.txt” This dataset contains the data of the playback experiment done with Drosophila pseudoobscura females from experimentally evolved populations to measure their preference function for one parameter of male courtship song (IPI; inter-pulse interval). # 9 populations of females were used: - E females: females from Elevated polyandry selection lines (6 males/1 female). There are 4 E populations: E1, E2, E3 and E4. - M females: females from Monogamy selection lines (1 male/1 female). There are 4 M populations: M1, M2, M3 and M4. - A females: females from the ancestral population # 4 artificial songs were used, varying in their IPI value: - EXAGG_IPI: exaggerated IPI (very short) - SHORT_IPI: average E male IPI (short) - LONG_IPI: average M male IPI (long) - PERS_IPI: persimilis IPI (very long) # Each row of the table corresponds to one of the females tested. The table contains the following columns: - playback_session: the session of the playback experiment (formed of the date + the artificial song used + the starting time of the experiment) - date_experiment: the date of the experiment (dd/mm/yyyy) - female_number: the female number in each chamber (from 1 to 10, the number 1 being the first mated) - time_start_experiment: the time the experiment started - playback_song: the artificial song used for the playback experiment - female_population: the population of the female (E1-4, M1-4 or A) - time_to_mate: the mating latency (in min). ‘NA’ is indicated when the female has not mated at the end of the 20 min experiment) - female_treatment: the sexual selection treatment of the female (E, M or A) - female_replicate: the replicate of the treatment used (1, 2, 3, 4 or A) - mated_or_not: the status of the female (0=not mated at the end of the 20 min experiment; 1=mated) ### MATING LATENCY ANALYSIS The column ‘time_to_mate’ was used as the response variable. The variable was first log-transformed. Only the first 5 mated individuals of each chamber were included in the analysis (female_number<6). ### MATING PROBABILITY ANALYSIS The column ‘mated_or_not’ was used as the response variable( in a logistic regression).