Pollen metabarcoding sequences used in: Anthony D. Vaudo, David J. Biddinger, Wiebke Sickel, Alexander Keller, and Margarita M. López-Uribe. Phylogenetic pollen preferences facilitate naturalization and pollination services of introduced bees in new habitats. Biology Letters Author emails: advaudo@gmail.com, djb134@psu.edu, wiebke.sickel@thuenen.de, a.keller@biozentrum.uni-wuerzburg.de, mml64@psu.edu Sequence .fastq files are demultiplexed ITS2 metabarcoded sequences, sequenced on Illumina MiSeq (Illumina, Inc., San Diego, CA, USA). "its2.penn-malus2018-5.fasta" is the reference ITS2 species sequences of flowering plants of Pennsylvania used for classification. Code for sequence quality filtering and classification are provided in "SI1_qc_classification.sh". Abundance and Taxonomic tables are provided as "combined.vs.otu" and "combined.vs.tax" respectively. Code for taxa filtering and normalization are provided in "SI2_normalize_filtertaxa_diversity_dataoutput.R". Final data for analysis is provided as "count_data_analysis.xlsx" Sample metadata are: Static Sample_ID Site Landscape 1 BR-1 B_Rice Woods 2 BR-2 B_Rice Woods 3 BR-3 B_Rice Woods 4 BR-4 B_Rice Woods 5 BR-5 B_Rice Woods 6 BR-6 B_Rice Woods 7 BR-7 B_Rice Woods 8 BR-8 B_Rice Woods 9 BR-9 B_Rice Woods 10 BR-10 B_Rice Woods 11 BR-11 B_Rice Woods 12 BR-12 B_Rice Woods 13 SF-1 S_Famous Urban 14 SF-2 S_Famous Urban 15 SF-3 S_Famous Urban 16 SF-4 S_Famous Urban 17 SF-5 S_Famous Urban 18 SF-6 S_Famous Urban 19 SF-7 S_Famous Urban 20 SF-8 S_Famous Urban 21 SF-9 S_Famous Urban 22 SF-10 S_Famous Urban 23 SF-11 S_Famous Urban 24 SF-12 S_Famous Urban 25 94-1 Lerew_94 Cherry 26 94-2 Lerew_94 Cherry 27 94-3 Lerew_94 Cherry 28 94-4 Lerew_94 Cherry 29 94-5 Lerew_94 Cherry 30 94-6 Lerew_94 Cherry 31 94-7 Lerew_94 Cherry 32 94-8 Lerew_94 Cherry 33 94-9 Lerew_94 Cherry 34 94-10 Lerew_94 Cherry 35 94-11 Lerew_94 Cherry 36 94-12 Lerew_94 Cherry 37 TH-1 Lerew_TH Cherry 38 TH-2 Lerew_TH Cherry 39 TH-3 Lerew_TH Cherry 40 TH-4 Lerew_TH Cherry 41 TH-5 Lerew_TH Cherry 42 TH-6 Lerew_TH Cherry 43 TH-7 Lerew_TH Cherry 44 TH-8 Lerew_TH Cherry 45 TH-9 Lerew_TH Cherry 46 TH-10 Lerew_TH Cherry 47 TH-11 Lerew_TH Cherry 48 TH-12 Lerew_TH Cherry 49 PT-1 Peters Cherry 50 PT-2 Peters Cherry 51 PT-3 Peters Cherry 52 PT-4 Peters Cherry 53 PT-5 Peters Cherry 54 PT-6 Peters Cherry 55 PT-7 Peters Cherry 56 PT-8 Peters Cherry 57 PT-9 Peters Cherry 58 PT-10 Peters Cherry 59 PT-11 Peters Cherry 60 PT-12 Peters Cherry 61 DV-1 Diverley Apple 62 DV-2 Diverley Apple 63 DV-3 Diverley Apple 64 DV-4 Diverley Apple 65 DV-5 Diverley Apple 66 DV-6 Diverley Apple 67 DV-7 Diverley Apple 68 DV-8 Diverley Apple 69 DV-9 Diverley Apple 70 DV-10 Diverley Apple 71 DV-11 Diverley Apple 72 DV-12 Diverley Apple 73 KFL-1 KFL Apple 74 KFL-2 KFL Apple 75 KFL-3 KFL Apple 76 KFL-4 KFL Apple 77 KFL-5 KFL Apple 78 KFL-6 KFL Apple 79 KFL-7 KFL Apple 80 KFL-8 KFL Apple 81 KFL-9 KFL Apple 82 KFL-10 KFL Apple 83 KFL-11 KFL Apple 84 KFL-12 KFL Apple 85 DSR-1 DS_Ramp Apple 86 DSR-2 DS_Ramp Apple 87 DSR-3 DS_Ramp Apple 88 DSR-4 DS_Ramp Apple 89 DSR-5 DS_Ramp Apple 90 DSR-6 DS_Ramp Apple 91 DSR-7 DS_Ramp Apple 92 DSR-8 DS_Ramp Apple 93 DSR-9 DS_Ramp Apple 94 DSR-10 DS_Ramp Apple 95 DSR-11 DS_Ramp Apple 96 DSR-12 DS_Ramp Apple