The table contains genotype data of the samples of Lychnis flos-cuculi collected from 17 populations in Oberaargau region, Switzerland. The primers for the amplification of the first three loci originate from Galeuchet et al. (2002): Cuculi 4, Cuculi 17 and Cuculi 19. Primers for amplifying SL_eSSR13, SL_eSSR17 and SL_eSSR49 originate from Moccia et al. 2009 and Molecular Ecology Resources (2010). A few samples did not amplify at some loci being marked as "0". Galeuchet DJ, Husi R, Perret C, Fischer M, Gautschi B (2002) Characterization of microsatellite loci in Lychnis flos-cuculi (Caryophyllaceae). Mol Ecol Notes 2: 491-492. Moccia MD, Oger-Desfeux C, Marais GAB, Widmer A (2009) A White Campion (Silene latifolia) floral expressed sequence tag (EST) library: annotation, EST-SSR characterization, transferability, and utility for comparative mapping. BMC Genomics 10: 243. Molecular Ecology Resources Primer Development Consortium (2010) Permanent Genetic Resources added to Molecular Ecology Resources Database 1 October 2009–30 November 2009. Mol Ecol Resources 10: 404-408.