The purpose of this READ ME file is to provide guidance to the data used in the Tree Genetics and Genomes publication: "A tapetal ablation transgene induces stable male-sterility and slows field growth in Populus". The authors are Estefania Elorriaga, Richard Meilan, Cathleen Ma, Jeffrey S. Skinner, Elizabeth Etherington, Amy Brunner, and Steven H. Strauss. The paper was accepted on July 18th 2014 and it was published online on August 13th 2014. The DRYAD file contains raw data gathered from a field trial of transgenic trees that were expressing a RNAse (barnase) in the tapetal layer of the anthers. The raw data encompasses: 1) An Excell spreadsheet named "Sterility_VolumeIndex" with growth data (height, diameter, volume) for the trees expressing the RNAse (sterility trial) 2) An Excell spreadsheet named "Reporter_VolumeIndex" with growth data for the trees that were part of a reporter trial 3) An Excell spreadsheet named "Catkin_length_weight" with length, weight, and angle of catkins taken from the sterility trial trees 4) An Excell spreadsheet named "Pollen_count" with pollen count data from the sterility trial trees 5) A text file name "R code" with the programing code used in R to perform the different statistical analyses needed for analysis of both the sterility trial and the reporter trial General notes: The Original genotype code refers to the original transgenic event number that was assigned during research. The Manuscript genotype code refers to the transgenic event number we selected for the manuscript for ease of reading. The equivalency among the two is given in the manuscript. ----->FILE: Sterility_VolumeIndex<----- This file has the volume index (i.e. height*diameter^2) data for the sterility trial. Note, we created the last column in order to have reasonable value on the y-axis of our volume index figure (Fig. 2). This figure can be found in the sheet "Volume index - Mean value". The row refers to the row in which the tree was located in the field. The same stands for block. R was also used to perform a Dunnett’s multiple comparison test for this data. We used the Dunnett's test results to obtain the 95% confidence intervals and the number of stars in our figure (Fig. 2). Figure 2 can be found in this Excel sheet. The code is also available here in the DRYAD file and it is named "R code.docx". ----->FILE: Reporter_VolumeIndex<----- This file has the volume index (i.e. height*diameter^2) data for the reporter trial. The R statistical computer language and environment version 3.0.1 was used to perform a Dunnett’s multiple comparison test. The Dunnett's test allowed us to compare the mean volume index of each construct against the control. The output of the Dunnett's test provided us with P-values, 95% confidence interval, and stars according to significance. We used thees results to obtain the 95% confidence intervals in this table. These results were used to create Fig. 3, which can be found in this sheet. ----->FILE: Catkin_length_weight<----- In this Excel file, you will find the length, weight, and angle data of the catkin samples per event for both harvest dates that were recorded, March 10th 2009 and March 14th 2009. The data presented was used to create Fig. S3 (included in the file), Fig. S4 (included in the file), Table S13, Table S14, and Table S15, and to calculate the values for average catkin angle that is reported in the manuscript. ----->FILE: Pollen_count<---- This excel sheet has the pollen grain count data for the control trees from the sterility trial. There is a sheet for every year (i.e. 2006, 2007, 2008, and 2009) that pollen grains were counted. Viability testing was not done on 2006 or 2007, so there are no viability values for these years. Note, in the yaer 2009 there were more samples than in 2008, so there are two more columns: Total average viable pollen grains and total average percent viable pollen. ----->FILE: R code<----- This file contains the R code used to perform Dunnett’s multiple comparison tests with the growth data from the sterility and reporter trials.