Read me file for "Implementing an evolutionary framework for understanding genetic relationships of phenotypically defined insect biotypes in the invasive soybean aphid (Aphis glycines)". For file "Master_GenePop.txt" The file is representative of an unparsed dataset including all sampled aphids and genotyped SNP loci. The file is in GenePop format, as was generated by GenAlEx. For file "Master_GenAlEx.txt" The file is representative of an unparsed dataset including all sampled aphids and genotyped SNP loci as. The file is in GenAlEx format, a software package utilized widely for our analyses. Both datasets have not had aphids of the same clonal lineage or loci undergoing selection removed. In order to replicate the dataset utilized within the study one must: 1. Analyze the data set for shared MLGs and reduce all shared MLG's within a single population to include only one member (delete all but one member of a shared MLG) 2. Remove loci 42701, 5109, 2654 as these were found to be undergoing strong selection (Lositan). Individual codes: Sampled individual soybean aphids are sorted by two part code (EX: P1A1_BIO1) The first two digits (P#) represent the geographic location of the sample: P1 = Defiance County P2 = Wood County P3 = Fulton County P4 = Wayne County Field 1 P5 = Wayne County Field 2 P6 = Wayne County Field 3 P7 = Wayne County Field 4 The next two two three digits (Letter, #, #) identify the sample too individual aphid by their location in the 96 well plates used for genotyping. The 4 digit code located after the underscore indicates the sample's biotype with Biotype 1 individuals marked BIO1, and biotype 2 with BIO2. Example: P1A1_BIO1 P1 - The aphid was sampled in Defiance County A1 - The individual aphid's DNA is stored in A1 of its genotyping plate. The individual ID#. BIO1 - The aphid belongs to biotype 1.