This README file refers to the data reported in manuscript 13-0899.R1, published in Evolution. Authors: Romain Rouchet & Christoph Vorburger Title: Experimental evolution of parasitoid infectivity on symbiont-protected hosts leads to the emergence of genotype-specificity Data were collected by both authors, for queries please contact christoph.vorburger@eawag.ch. The experimental design is explained in the publication. 1) The table "infectivity_gen0" corresponds to the infectivity test at the beginning of experimental evolution procedure and contains the following columns: rep_id: Replicate identifier aphid_subline: Aphid subline in the infectivity test consisted of four sublines: the H. defensa-free clone A06-407 and the three H. defensa-infected sublines of the same clone: A06-407H323, A06-407H402 and A06-407H76. evolution_treatment: Evolution treatment consisted of the original parasitoid population created at the beginning of the experimental evolution procedure. n_nymph: Number of 48-72h old aphid nymphs from one of the aphid sublines in each replicate. n_mummy: Number of mummified aphids after 11 days. n_survivor: Number of aphids still alive after 11 days. n_dead: Number of aphids dead after 11 days. p_mummy: Proportion of mummified aphids, i.e. the number of mummies divided by the number of nymphs in each replicate. Measure of infectivity. 2) The table "infectivity_gen5" corresponds to the infectivity test after five generations of experimental evolution and contains the following columns: rep_id: Replicate identifier aphid_subline: Aphid subline in the infectivity test consisted of four sublines: the H. defensa-free clone A06-407 and the three H. defensa-infected sublines of the same clone: A06-407H323, A06-407H402 and A06-407H76. evolution_treatment: Evolution treatment consisted of four aphid treatments in the experimental evolution procedure: the H. defensa-free clone A06-407 and the three H. defensa-infected sublines of the same clone: A06-407H323, A06-407H402 and A06-407H76. evolution_line: Evolution line consisted of the four replicates for each of the evolution treatment. n_nymph: Number of 48-72h old aphid nymphs from one of the aphid sublines in each replicate. n_mummy: Number of mummified aphids after 11 days. n_survivor: Number of aphids still alive after 11 days. n_dead: Number of aphids dead after 11 days. p_mummy: Proportion of mummified aphids, i.e. the number of mummies divided by the number of nymphs in each replicate. Measure of infectivity. 3) The table "infectivity_gen11" corresponds to the infectivity test after eleven generations of experimental evolution and contains the following columns: rep_id: Replicate identifier aphid_subline: Aphid subline in the infectivity test consisted of four sublines: the H. defensa-free clone A06-407 and the three H. defensa-infected sublines of the same clone: A06-407H323, A06-407H402 and A06-407H76. evolution_treatment: Evolution treatment consisted of four aphid treatments in the experimental evolution procedure: the H. defensa-free clone A06-407 and the three H. defensa-infected sublines of the same clone: A06-407H323, A06-407H402 and A06-407H76. evolution_line: Evolution line consisted of the four replicates for each of the evolution treatment. n_nymph: Number of 48-72h old aphid nymphs from one of the aphid sublines in each replicate. n_mummy: Number of mummified aphids after 11 days. n_survivor: Number of aphids still alive after 11 days. n_dead: Number of aphids dead after 11 days. p_mummy: Proportion of mummified aphids, i.e. the number of mummies divided by the number of nymphs in each replicate. Measure of infectivity. 4) The table "infectivity_mixed_lines" corresponds to the infectivity test after the mixing of the experimental lines and contains the following columns: rep_id: Replicate identifier aphid_subline: Aphid subline in the infectivity test consisted of four sublines: the H. defensa-free clone A06-407 and the three H. defensa-infected sublines of the same clone: A06-407H323, A06-407H402 and A06-407H76. evolution_treatment: Evolution treatment consisted of four aphid treatments in the experimental evolution procedure: the H. defensa-free clone A06-407 and the three H. defensa-infected sublines of the same clone: A06-407H323, A06-407H402 and A06-407H76. n_nymph: Number of 48-72h old aphid nymphs from one of the aphid sublines in each replicate. n_mummy: Number of mummified aphids after 11 days. n_survivor: Number of aphids still alive after 11 days. n_dead: Number of aphids dead after 11 days. p_mummy: Proportion of mummified aphids, i.e. the number of mummies divided by the number of nymphs in each replicate. Measure of infectivity. 5) The table "traits_evolution_lines" corresponds to the life-history traits (sex, mass, tibia length, development time) measured in the evolution lines and contains the following columns: id_sample: Sample identifier. Correspond to the individual parasitoid assayed. replicate: Replicate. Each replicate correspond to one aphid colony and one parasitoid female from which assayed parasitoids were produced. evolution_treatment: Evolution treatment consisted of four aphid treatments in the experimental evolution procedure: the H. defensa-free clone A06-407 and the three H. defensa-infected sublines of the same clone: A06-407H323, A06-407H402 and A06-407H76. evolution_line: Evolution line consisted of the four replicates for each of the evolution treatment. sex: Sex of adult parasitoids. mass_mg: Fresh mass of adult parasitoids in mg, measured within 24 hours after emergence. tibia_mm: Tibia size in mm. devtime_d: Parasitoid development time (until emergence) measured in days. 6) The table "eggs_evolution_lines" corresponds to the average egg size measured in the evolution lines and contains the following columns: id_sample: Sample identifier. Correspond to the individual parasitoid assayed evolution_treatment: Evolution treatment consisted of four aphid treatments in the experimental evolution procedure: the H. defensa-free clone A06-407 and the three H. defensa-infected sublines of the same clone: A06-407H323, A06-407H402 and A06-407H76. evolution_line: Evolution line consisted of the four replicates for each of the evolution treatment. eggsize_um: Average egg size per female (measured in 10 eggs per female) in micrometer. 7) The table "traits_mixed_evolution_lines" corresponds to the life-history traits (sex, mass, tibia length, development time) measured in the evolution treatment after mixing the evolution lines and contains the following columns: id: Sample identifier. Correspond to the individual parasitoid assayed. replicate: Replicate. Each replicate correspond to one aphid colony and one parasitoid female from which assayed parasitoids were produced. evolution_treatment: Evolution treatment consisted of four aphid treatments in the experimental evolution procedure: the H. defensa-free clone A06-407 and the three H. defensa-infected sublines of the same clone: A06-407H323, A06-407H402 and A06-407H76. sex: Sex of adult parasitoids. mass_mg: Fresh mass of adult parasitoids in mg, measured within 24 hours after emergence. tibia_mm: Tibia size in mm. devtime_d: Parasitoid development time (until emergence) measured in days. 8) The table "reduced_infectivity_mixed" corresponds to the infectivity on the H. defensa-free clone A407 of the parasitoids from the different evolution treatment after the mixing of the evolution lines and contains the following columns: id_rep: Replicate identifier a_subline: Aphid subline in the infectivity test consisted of four sublines: the H. defensa-free clone A06-407 and the three H. defensa-infected sublines of the same clone: A06-407H323, A06-407H402 and A06-407H76. evolution_treatment: Evolution treatment consisted of four aphid treatments in the experimental evolution procedure: the H. defensa-free clone A06-407 and the three H. defensa-infected sublines of the same clone: A06-407H323, A06-407H402 and A06-407H76. n_nymph: Number of 48-72h old aphid nymphs from one of the aphid sublines in each replicate. n_mummy: Number of mummified aphids after 11 days. n_survivor: Number of aphids still alive after 11 days. n_dead: Number of aphids dead after 11 days. p_mummy: Proportion of mummified aphids, i.e. the number of mummies divided by the number of nymphs in each replicate. Measure of infectivity. Notes: 'NA' is used for missing data.