This dataset contains some of the data from: MacLellan, K., L. Kwan, M.C. Whitlock, and H.D. Rundle. 2011. Dietary stress does not strengthen selection against single deleterious mutations in Drosophila melanogaster. Heredity: in press. Data collectors: Kelsie MacLellan Lucia Kwan Howard Rundle, contact for questions: e-mail: hrundle@uottawa.ca All the data for this publication are contained within three files, each saved as CSV file (i.e. values separated by commas). There are no missing values. This is the third of the three. The file (rundle3_data.csv) contains the data from the productivity assay that counted the total number of adult offspring that emerged from the eggs laid by a single female in a 24 hr period when mated to a randomly chosen stock male. Replicate females were raised in the ancestral (cornmeal) and novel (corn-flour) food environments and laid eggs in the same environment in which they were raised. Productivity was measured in blocks such that females from two mutant populations and the stock were assayed in both environments within any particular generation. In creating the females for use in this assay, two mutant and the stock population were raised together in a common vial for one generation prior to the assay (Fig. 1 in the manuscript). Four female offspring were collected as virgins upon emergence from a particular mixed vial and used in the productivity assay: two stock females and one female of each of the two mutants. Each row in the data file gives the productivity of a single mutant female and the two stock females raised in the same vial. A separate row gives the productivity of the other mutant female raised in the same vial, along with the same two stock females. Data for each pair of stock females thus appear twice in the data file. The dataset has following columns: 1) Mutation. The identity of the mutant female in question. 2) environment. The environment in which the mutant and stock females were raised and in which their productivity was measured. 3) block. The experiment was performed across five blocks. 4) replicate. Replicate number within each mutation and environment. There is no correspondence of replicate numbers across mutations and environments. 5) mutant productivity. Total number of adult offspring produced by the mutant female. 6) control productivity 1. Total number of adult offspring produced by the first of two stock females. 7) control productivity 2. Total number of adult offspring produced by the second of two stock females. 8) mean control productivity. The average of columns 6 and 7. 9) difference. Column 5 - column 8.