The nexus files (ChB6align, IAPalign, IL2align, TGFB3align, and TRAILalign) are alignments of greater prairie-chicken immune gene sequences from six populations. The five immune genes are chicken B-cell marker 6 (ChB6), interleukin-2 (IL2), transforming growth hormone beta 3 (TGFB3), inhibitor of apoptosis protein-1 (IAP), and tumor necrosis factor-related apoptosis inducing ligand-like protein (TRAIL). The six populations are Wisconsin (denoted by "BV" for Buena Vista subpopulation), Nebraska (NE), Minnesota (MN), Missouri (MO), Kansas (KS), and Texas (APC, for Attwater's prairie chicken). Each individual is represented by two seqeunces (the two phased haplotypes).