This file explains all of the variables in each of the datasets that accompany: Johnson et al. 2017 ms. Evidence that fertility trades off with early offspring fitness as males age Cohort-based longitudinal analyses: dataset: MaleSurvival.csv Age = age of males when sampling (months) Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3 Survived = number of males alive at sampling x.Survived = number of males dad at sampling (from total of 15) P_Survived = proportion of males surviving dataset: MatingSuccess.csvÊ Age = age of males when sampling (months) Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3 Mated = number of surviving males that mated (i.e., fertilised eggs produced) during mating interval x.Mated = number of surviving males that did not mate during mating interval P_Mated = proportion of males that mated dataset: SpermProduction.csv Age = age of males when sampling (months) Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3 VOL = male ejaculate volume, categorised into 4 levels (0=no semen, 1=0-1 uL, 2=1-3 uL, 3=3+ uL) VOL_binary = male ejaculate collected at sampling (0 = no, 1 = yes) dataset: SpermConcentration.csv Age = age of males when sampling (months) Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3 FishID_unique = male ID, a combination of the age at sampling, the line (ABZ) and the Fish ID Sperm_mL = concentration of sperm per mL Size = total male length (cm) dataset: CASA.csv Age = age of males when sampling (months) Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3 FishID_unique = male ID, a combination of the age at sampling, the line (ABZ) and the Fish ID VAP = sperm swimming speed (actual path velocity) VSL = sperm swimming speed (straight line velocity) VCL = sperm swimming speed (curvilinear velocity) MOTILE_PCT = percentage of motile sperm in sample Size = total male length (cm) dataset: IVF.csvÊ Age = age of males when sampling (months) Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3 FishID_unique = male ID, a combination of the age at sampling, the line (ABZ) and the Fish ID Developed = number of developed embryos at 24 hours post fertilisation x.Developed = number of undeveloped embryos at 24 hours post fertilisation P_Developed = sperm swimming speed (curvilinear velocity) Hatch = number of developed embryos (@ 24 hpf) that hatched by 5 days x.Hatch = number of developed embryos that did not hatch by 5 days P_Hatch = proportion of developed embryos (@ 24 hpf) that hatched by 5 days Size = total male length (cm) dataset: ProgenySurvival.csvÊ Age = age of males when sampling (months) Tank = tank fish housed in, ML6B1, ML6B2 or ML6B3 FishID_unique = male ID, a combination of the age at sampling, the line (ABZ) and the Fish ID Survived = number of developed embryos (@ 24hpf) that survived to 15 days x.Survived = number of developed embryos (@ 24 hpf) that did not survive to 15 days P_Survived = sperm swimming speed (curvilinear velocity) Cross-sectional (young vs. old males) analyses: dataset: CASA_CS.csv Age = age of males when sampling (months) FishID_unique = male ID VAP = sperm swimming speed (actual path velocity) VSL = sperm swimming speed (straight line velocity) VCL = sperm swimming speed (curvilinear velocity) MOTILE_PCT = percentage of motile sperm in sample dataset: IVF_CS.csvÊ Age = age of males when sampling (months) FishID_unique = male ID Developed = number of developed embryos at 24 hours post fertilisation x.Developed = number of undeveloped embryos at 24 hours post fertilisation P_Developed = sperm swimming speed (curvilinear velocity) Hatch = number of developed embryos (@ 24 hpf) that hatched by 5 days x.Hatch = number of developed embryos that did not hatch by 5 days P_Hatch = proportion of developed embryos (@ 24 hpf) that hatched by 5 days dataset: ProgenySurvival_CS.csvÊ Age = age of males when sampling (months) FishID_unique = male ID Survived = number of developed embryos (@ 24hpf) that survived to 15 days x.Survived = number of developed embryos (@ 24 hpf) that did not survive to 15 days P_Survived = sperm swimming speed (curvilinear velocity)