Readme file for Colyott, K., C. Odu, and J.M. Gleason. 2016. Dissection of signaling modalities and courtship timing reveals a novel signal in Drosophila saltans courtship. Animal Behaviour. In press. This read file describes the data files accompanying the above publication. For any further queries please contact KColyott@KU.edu or jgleason@ku.edu The following files are included; 1) “Dsaltans_light_dark_AB.csv” -Methods of data collection are in the Vision section of manuscript 2) “Dsaltans_eye_ablation_AB.csv” -Methods of data collection are in the Vision section of manuscript 3) “Dsaltans_wing_ablation_AB.csv” - Methods of data collection are in the Wing removal section of manuscript 4) “Dsaltans_aristae_ablation_AB_.csv” -Methods of data collection are in the Aristae removal section of manuscript 5) “Dsaltans_antennae_ablation_AB.csv” -Methods of data collection are in the Olfaction section of manuscript 6) “Dsaltans_foretarsi_ablation_AB.csv” -Methods of data collection are in the Gustation section of manuscript 7) “Dsaltans_midtarsi_ablation_AB.csv” -Methods of data collection are in the Midtarsi section of manuscript Description of data included in each file: 1) “Dsaltans_light_dark_AB.csv” Col1: Larvae - yes or no Col2: Treatment - light or dark Col3: Light 1=yes, 0=no Col4: Dark 1=yes, 0=no Col5: Larvae_0-1 1=yes, 0=no 2-7) “Dsaltans_eye_ablation_AB.csv”, “Dsaltans_wing_ablation_AB.csv”, “Dsaltans_aristae_ablation_AB_.csv”, “Dsaltans_antennae_ablation_AB.csv”, “Dsaltans_foretarsi_ablation_AB.csv” and “Dsaltans_midtarsi_ablation_AB.csv” Col1: Male blind 1=yes, 0=no Col2: Female blind 1=yes, 0=no Col3: Courtship 1=yes, 0=no Col4: Copulation 1=yes, 0=no Col5: TMT 1= male control + female control, 2= male control + female ablated, 3= male ablated + female control, 4= male ablated + female ablated Col6: CourtLat latency to courtship in hour:min:sec Col7: CourtLatSec latency to courtship in seconds - missing data is males that failed to court during experiment Col8: CopLat latency to copulation in hour:min:sec Col9: CourtDurSec duration of courtship in seconds (calculated by CopLat- CourtLat) - missing data is males that failed to court during experiment Col10: CopDur duration of copulation in hour:min:sec - not used because of high amount of missing data for some treatments Col11: CopDurSec duration of copulation in seconds - not used because of high amount of missing data for some treatments -Methods of data analysis are in Data Analysis section of manuscript