The file presents data underlying the analyses for an article called Serum-free and Xenobiotic-free Preservation of Cultured Human Limbal Epithelial Cells in preparation for publication in PlosOne. For all tables, column C describes the experimental groups, column D gives the number of the experiment as referred to in the article and column E gives the name of each sample analyzed. The table called ?Cell Viability? contains the percentage of viable cells per sample calculated as the number of green cells divided with the total number of cells (green + red). The percentages are found from averaging countings from 5 randomly picked areas of the basal layer of the cultured human limbal epithelial sheets, assessed by confocal microscopy and a Calcein/Ethidium Homodimer viability kit. The table called "Epithelial Thickness" contains the thickness of the epithelial sheets (amniotic membrane not included) in micrometers measured on four pre-set spots; 250, 500, 750 and 1000 micrometers from the explant (column F, G, H and I) of each sample. Column J gives the average of the four pre-set spots per culture. The table called "Aminotic Membrane Thickness" contains the thickness of the amniotic membrane in micrometers measured on four pre-set spots; 250, 500, 750 and 1000 micrometers from the explant (column F, G, H and I) of each sample. Column J gives the average of the four pre-set spots per culture. The remaining tables contain data of the expression of 11 different immunohistochemical markers, each table being named after the marker presented (deltaNP63alpha, P63, Bmi-1, CEBPdelta, ABCG2, Connexin-43, Keratin-19, Keratin-3, PCNA, Caspase-3). The immunohistochemical marker tables contain calculations from two independent investigators, called investigator 1 (column F through H) and investigator 2 (column J through M). The actual numbers of positive and negative cells with respect of marker expression are presented in column F and G for Investigator 1 and column I and J for Investigator 2. The results from the two investigators are averaged in column L. 3 %. Samples with less than 50 cells were not included in the calculations, and these samples were noted as missing. Consult the article for further information regarding materials, methods and results. Questions can be sent to corresponding author and group leader Tor Paaske Utheim, MD, PhD, Department of Medical Biochemistry, Oslo University Hospital, PO box 4956 Nydalen, 0424 Oslo, Norway. Email: utheim2@gmail.com.