File Pansu_gh_raw_data.txt This file contains raw sequences produced by g (5'-GGGCAATCCTGAGCCAA-3') and h (5'-CCATTGAGTCTCTGCACCTATC-3') primers. The sequences have been produced by the Illumina technology (HiSeq 2500 platform, 2x100bp pair-end). First filtering steps were performed using the OBITOOLS software (http://metabarcoding.org/obitools). Direct and reverse reads corresponding to the same sequence were aligned and merged thanks to the IlluminaPairEnd program. Only merged sequences with a high alignment quality score were retained (>=40). Each merged sequence was assigned to its original sample using the tags information previously added to primers thanks to the ngsfilter program. Only sequences containing both primers (with a maximum of 3 mismatches per primer) and exact tag sequences were selected. Sequences containing ambiguous nucleotides or shorter than 7 bp were discarded. Strictly identical sequences were merged together. Pure singleton (sequences with 1 read among the whole dataset) were removed. Data collection: Johan Pansu, Charline Giguet-Covex, Fabien Arnaud, Jerome Poulenard, Pierre Taberlet and Ludovic Gielly. Charline Giguet-Covex, Fabien Arnaud and Jerome Poulenard collected lake sediment cores in the field. Johan Pansu, Charline Giguet-Covex, ludovic Gielly collected lake sediment samples in the laboratory. Johan Pansu, Charline Giguet-Covex and Ludovic Gielly performed the DNA extraction. Ludovic Gielly and Charline Giguet-Covex performed the DNA amplification. Johan Pansu performed sequences filtering. Contact authors: Johan Pansu and Charline Giguet-Covex (johan.pansu@gmail.com ; charlinegiguet@gmail.com;) Column heading: sample:ANT01A1a: number of reads of the relevant sequence for sample ANT01A1a sample:ANT01A1b: see above (A and B of ANT01 = sample replicates, 1 and 2 of ANT01 = extraction replicates, a and b of ANT01 = PCR replicates) sample:ANT01A2a: see above (A and B of ANT01 = sample replicates, 1 and 2 of ANT01 = extraction replicates, a and b of ANT01 = PCR replicates) sample:ANT01A2b: see above (A and B of ANT01 = sample replicates, 1 and 2 of ANT01 = extraction replicates, a and b of ANT01 = PCR replicates) sample:ANT01B1a: see above (A and B of ANT01 = sample replicates, 1 and 2 of ANT01 = extraction replicates, a and b of ANT01 = PCR replicates) sample:ANT01B1b: see above (A and B of ANT01 = sample replicates, 1 and 2 of ANT01 = extraction replicates, a and b of ANT01 = PCR replicates) sample:ANT01B2a: see above (A and B of ANT01 = sample replicates, 1 and 2 of ANT01 = extraction replicates, a and b of ANT01 = PCR replicates) sample:ANT01B2b: see above (A and B of ANT01 = sample replicates, 1 and 2 of ANT01 = extraction replicates, a and b of ANT01 = PCR replicates) ... sample:TEMEXTx: number of reads of the relevant sequence for the xth extraction control (and two PCR for each extraction) sample:TEMPCRx or TEMVIDx : number of reads of the relevant sequence for PCR control Sequence_name : name of the sequence Count : number of reads of the sequence in the dataset Occurence : number of occurence among samples Sequence : DNA sequence