The zip file contains all the technical and biological replicates for the experiment with microbial biofilms taken from six sites in the Swiss stream MonchaltorferAa. Measurement were taken with a Gallios flow-cytometer and are presented in.fcs and .csv format. File name description: SamplingSite# - BiologicalReplicate#-year-month-day-UniqueRunNumber-Position.fcs and SamplingSite# - BiologicalReplicate#-year-month-day-UniqueRunNumber-PositionA.csv Example: A1-2-2015-03-19-154440-1.fcs Example explained in detail: A1 - Site 1 used for sampling (altogether six sites were used A1-A6) -2- - biological replicate (three samples were taken from each site, each from a different biofilm) -2015-03-19- need no explanation -154440- this ID is given by the flow-cytometer to each measurement run -3- position 3 (not relevant for this dataset) FCS files Standard fcs files contain raw data acquired with the Gallios flow cytometer. csv files are exports from the fcs files. Each row represent a single particle (cell) being measured, while the columns represent individual measurements (in particular the signal area for each measurement). the columns are: forward scatter, side scatter, FL1-10 (measurement of autofluorescence of the particle/cell at a particular wavelength - see associated paper methods for details).