Title: Phage gene expression and host responses lead to infection-dependent costs of CRISPR immunity Names: Sean Meaden, Loris Capria, Ellinor Alseth, Sylvain Gandon, Ambarish Biswas, Luca Lenzi, Stineke van Houte & Edze R. Westra Email: S.Meaden@exeter.ac.uk E.R.Westra@exeter.ac.uk Files: acr_KO_phage_comp_expt.csv Competition experiment between PA14 carrying 2 spacers targeting dms3vir phage and a lacZ marked surface mutant. Carried out with wt phage and phage lacking acr operon. Relative fitness determined based on blue/white colony counts. Variables: relative_fitness: Relative fitness of spacer carrying PA14 strain (BIM2). phage_genotype: wt phage dms3vir or dms3vir lacking acr operon. titer: phage titer used for experiment. replicate: 6 biological replicates labelled accordingly. cas1_comp_assay.csv Competition experiment between cas1 deletion mutant, WT PA14 or strain carrying 2 spacers (BIM2) vs. lacZ marked surface mutant. Relative fitness determined based on blue/white colony counts. Variables phage_titre_pfu_ml: phage titer used relative_fitness: fitness of tested strains relative to lacZ marked surface mutant. genotype: strain used in competition experiment. evo_expt_phage_data.csv Phage titres measured during the evolution experiment for each microcosm. Variables: Time: Days post start of experiment. PFU_per_ml: PFU per ml. treatment: Infection regime. D = daily infection. replicate: 12 biological replicates. evo_expt_pheno_data.csv Phentypic data based on streak assay against acr phage or wt. phenotype: sm (surface mutant), sens (sensitive), crispr (acquired 1 or more spacer) day: Days from start of infection treatment: D (daily reinfection regime). replicate: 12 biological replicates frequency: 24 colonies screened per microcosm, frequency is from 24 colonies, but in rare cases fewer were counted (discarded if ambiguous). protease_growth_rates.csv Comparison of PA14 straing carrying arabinose inducible protease gene vs. empty vector control. Measured via OD600 on a spectrophotometer. Rate determined by change in OD during log phase growth. Variables: well: Well in 96 well plate genotype: pro (protease expressing plasmid), ev (empty vector) ara_conc: 1% concentration rate: change in od600 during log phase.