This test of perfluorodecalin to increase transdermal oxygen delivery in mice readme.txt file was generated on 2021-03-05 by Lars Kaestner GENERAL INFORMATION 1. Title of Dataset: TRANSDERMALOXYGEN 2. Author Information Principal Investigator Contact Information Name: Lars Kaestner Institution: Saarland University Address: Campus E2 6, 66123 Saarbruecken, Germany Email: lars_kaestner@me.com 3. Date of data collection (range): 2020-06-19 to 2020-08-20 4. Geographic location of data collection: Saarland University, Campus University Hospital, Building 65, 66421 Homburg, Germany 5. Information about funding sources that supported the collection of the data: The study was supported by the Volkswagen Foundation in the framework 'Off the beaten track' and the European Framework Horizon 2020 under grant agreement number 860436 (EVIDENCE). SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: none 2. Links to publications that cite or use the data: not yet available 3. Links to other publicly accessible locations of the data: n/a 4. Links/relationships to ancillary data sets: n/a 5. Was data derived from another source?: no DATA & FILE OVERVIEW 1. File List: heart rate protocol 1.csv: data provide the heart rate of the mice (per minute) in dependence of the oxygen supplied (%) according to protocol 1, described in the methods oxygenation protocol 1.csv: data provide the oxygenation of the mice (%) in dependence of the oxygen supplied (%) according to protocol 1, described in the methods heart rate protocol 2.csv: data provide the heart rate of the mice (per minute) in dependence of the experimental time (min) according to protocol 2, described in the methods oxygenation protocol 2.csv: data provide the oxygenation of the mice (%) in dependence of the experimental time (min) according to protocol 2, described in the methods 2. Relationship between files, if important: the files 'heart rate protocol 1.csv' and 'oxygenation protocol 1.csv' belong to the same set of experiments and the files 'heart rate protocol 2.csv' and 'oxygenation protocol 2.csv' belong to the same set of experiments 3. Additional related data collected that was not included in the current data package: n/a 4. Are there multiple versions of the dataset?: no METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: Experiments with mice were carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The animal experiment protocol was approved by the State Office for Health and Consumer Protection (permit number: 08/2020). All efforts were made to ameliorate harm to the animals. This was achieved by performing the entire experimental procedure with the mice in anesthesia and by providing an additional pain therapy (details are provided below). For this study, we used sex mixed population of CD1nu/nu mice (Charles River, Wilmington, MA, USA) at an age of 50.3±8.5 weeks with a bodyweight of 29.9±3.7 g, which were housed under a 12h/12h day-night rhythm in the animal husbandry of the Institute for Clinical & Experimental Surgery (Homburg, Germany). The animals were kept in individually ventilated cages (IVCs) in groups of 5 animals per cage. They had free access to drinking water and standard pellet food (Altromin, Lage, Germany). No additional inclusion criteria were set. Animals were randomly distributed in 2 groups (control group and treated group). Randomization was done by choosing lots and experiments were performed by alternating measurements of control and treated mice with a cage of animals per (experimental) day. The number of animals per group could not be precalculated because we established this kind of measurements and had no reliable information about the variances of the measured parameters. Therefore, we performed a statistical analysis on a daily base and such decided after we reached a group size of 5 animals to stop the experiments of protocol 1 and after reaching 4 mice for protocol 2. During the experiment, the mice were anesthetized with isoflurane (5%: initiation, 1%: maintenance of anesthesia). Immediately after anesthesia initiation, the mice received 5 mg/kg carprofen (Rimadyl; Zoetis, Berlin, Germany) i.p. for intraoperative pain therapy. Afterwards, they were fixed on a heating pad in supine position and tracheotomized for the insertion of a catheter for ventilation. Breathing rate and stroke volume could be set on the mouse ventilator (Minivent; Harvard Apparatus, March-Hugstetten, Germany) and was adapted according to the body weight of the mice in the range of 200-270 per minute and 120-140 µL, respectively. The body temperature was kept constant in the range between 37-38oC, which was monitored with an anal probe (GTH1170, Greisinger Messtechnik, Regenstauf, Germany). The heart rate was documented with the help of a pulse oximeter (MouseSTAT Jr. with Paw sensor, Kent Scientific, Torrington, CT, USA.) The control group contained 5 animals at an age of 47.4±2.4 weeks with a body weight of 28.8±3.7 g and the test group contained the same number of animals (n=5 , age: 49.3±3.3 weeks, body weight: 29.4±4.9 g; no significant difference to control group). All mice in all groups and all protocols were given an initial acclimatization phase as outlined in Figure 1B (-10 to 0 min). Mice of control and treatment groups experienced the same hypoxic protocol. Control mice received no additional treatment whereas mice of the treatment group were brushed with prewarmed (30oC) perfluorodecalin (medical grade, Pharmpur, Königsbrunn, Germany). This included a full-body application with the exception of the area of the head and the paw to which the sensor of the pulse oximeter was attached. The procedure was continuously renewed in the course of the experiment to compensate the perfluorodecalin evaporation. The oxygenation of the blood was monitored and documented (in addition to the heart rate) with the pulse oximeter. After the oxygen saturation had reached a stationary level, the oxygen content of the ventilation was reduced according to the test protocol (see below). For this purpose, a gas mixer (KM10-2 FLEX, Witt-Gastechnik, Witten, Germany) was used, which mixed oxygen and nitrogen in an adjustable ratio. In addition, the setting was monitored with an oxygen meter (GOX100, Greisinger Messtechnik, Regenstauf, Germany). The mouse group used for protocol 2 consisted of 4 mice at an age of 55±15 weeks with a body weight of 31.9±1.1 g. 2. Methods for processing the data: All data were read on the displays of the devices specified above and hand-noted by the experimenter. 3. Instrument- or software-specific information needed to interpret the data: n/a DATA-SPECIFIC INFORMATION FOR: heart rate protocol 1.csv 1. Number of variables: 2 2. Number of cases/rows: 10 3. Variable List: oxygen supply, percentage of oxygen supplied in the mouse ventilation machine, % heart rate, heart rate of the mouse given by the pulse oximeter, /min 4. Missing data codes: n/a - data are not available 5. Specialized formats or other abbreviations used: none DATA-SPECIFIC INFORMATION FOR: oxygenation protocol 1.csv 1. Number of variables: 2 2. Number of cases/rows: 10 3. Variable List: oxygen supply, percentage of oxygen supplied in the mouse ventilation machine, % oxygenation, oxygenation of the mouse given by the pulse oximeter, % 4. Missing data codes: n/a - data are not available 5. Specialized formats or other abbreviations used: none DATA-SPECIFIC INFORMATION FOR: heart rate protocol 2.csv 1. Number of variables: 2 2. Number of cases/rows: 4 3. Variable List: time, experimental time, min heart rate, heart rate of the mouse given by the pulse oximeter, /min 4. Missing data codes: n/a - data are not available 5. Specialized formats or other abbreviations used: none DATA-SPECIFIC INFORMATION FOR: oxygenation protocol 2.csv 1. Number of variables: 2 2. Number of cases/rows: 4 3. Variable List: time, experimental time, min oxygenation, oxygenation of the mouse given by the pulse oximeter, % 4. Missing data codes: n/a - data are not available 5. Specialized formats or other abbreviations used: none