Supporting data for "Breakdown of Phylogenetic Signal: A Survey of Microsatellite Densities in 454 Shotgun Sequences from 154 Non Model Eukaryote Species" by Emese Meglecz, Gabriel Neve, Ed Biffin and Michael G. Gardner (PLoS ONE,2012) ######################################## Fasta files: One fasta file for each species, with 454 reads of a partial non-enriched genomic library. Sequences do not contain adaptors of sample tags. Following Gardner et al. [58], DNA from all species was sheared by CovarisTM and 500 ng of purified DNA was used for 454 FLX Titanium library (Roche Applied Science) preparation, according to the manufacturer’s protocols using parallel sample cleanup and RL MID adapters. Emulsion PCR (emPCR) was carried out at a ratio of three copies per bead. Each Titanium PicoTiter plate contained two gaskets and two million beads were loaded in each half which was the equimolar pooling of libraries from 2-4 species in each of them. Sequencing was done with 200 cycles. Sample preparation and analytical processing, such as base calling, were performed at Australian Genome Research Facility Ltd (AGRF, Brisbane Australia), according to the manufacturer’s protocol for the Titanium series. ########################################