## DATASET INFO ## Dataset Title: Drosophila wing disc cell morphology in timelapse Contact info: Natalie Dye, MPI-CBG, Pfotenhauerstrasse 108, 01307 Dresden, dye@mpi-cbg.de Alternate contact: Marko Popovic, MPI-PKS, Nöthnitzer Str. 38, 01187 Dresden, mpopovic@pks.mpg.de Date of data collection: raw data ranging from Oct, 2014 to Dec, 2016; tables last generated in May 2020 Location of data collection: Dresden, Germany Keywords: Drosophila, wing, cell morphology, cell area, cell elongation, tissueminer Funding: Deutsche Forschungsgemeinschaft, Max Planck Society ## Data overview ## These tables include measurements of cell morphology over time in the proliferating Drosophila larval wing disc, specifically the wing disc pouch. Five wing disc samples were grown in ex vivo culture using conditions described in Dye et al 2017, with images acquired every 5 min for ~13 hrs. Images were segmented and analyzed with TissueMiner (Etournay et al 2016). The datasets are used in Dye et al 2017 and Dye et al 2020. The tables provided here include measurements of cell morphology (area and elongation) at each timepoint in each of the 5 wing discs. For cell elongation, we report the elongation values for each of its component triangles, formed by connecting the cell center to the center of each its neighbors (see Etournay et al 2016). We also provide a table that reports the x,y position of a point calculated as the center of radial cell elongation (see Dye et al 2020). Note that wing disc is not exactly flat but rather a dome. Here, we have also taken into account the local curvature of the disc and corrected for distortions associated with forcing our analysis to 2D. Units are generally in pixels (0.2um/pixel). ## Methods: ## Data were acquired and processed by Natalie A. Dye, Dresden Germany, 2014-2020 Data collection: Timelapse movies of E-cadherin-GFP expressing wing discs were acquired as described in Dye et al 2017. Briefly, they were grown in Grace's media containing 20nM 20-hydroxyecdysone in a glass-bottom dish covered with a porous filter (using a double-stick tape spacer) at 25C. We used a spinning disc microscope to acquire Z-stacks (0.5um Z-steps, 0.2x0.2 um/pixel in x and y). Data processing: For each movie, we projected the apical surface onto a 2D surface for segmentation. TissueAnalyzer (FIJI plugin) was used to segment and track each cell over time, and TissueMiner (Etournay et al 2016) was used for further analysis. We corrected cell area and elongation for local curvature as described in Dye et al 2017 and Dye et al 2020 (denoted "height-corrected" below). For each movie, we also calculated the center of radial cell elongation symmetry, as described in Dye et al 2020. ## File Overview: ## Figure1_SourceData_CellArea_EachMovie_AllTimes.csv Cell area values (in square pixels) for each cell of each movie at each timepoint (frame). Generated 2020.05.05 Figure1_SourceData_TriangleElongation_EachMovie_AllTimes.csv Triangle elongation for each movie at each timepoint (frame). Generated 2020.05.05 Figure1_SourceData_CenterPositions_EachMovie.csv Calculated center points for the tissue-wide radial cell elongation pattern (pixels in x, y in image coordinates) for each movie. Generated 2020.05.05 ### File-specific information ### Figure1_SourceData_CellArea_EachMovie_AllTimes.csv Columns: movie: name of the movie. Ecd means it was acquired with 20nM 20hE, date of acquisition, F or P + # indicates the position (usually 2 discs were acquired each day, but only the one that didn't move or looked the brightest was taken). frame: frame # of the timelapse time_hr: actual elapsed time since start of movie, in hours (taken from the metadata of the microscope). cell_id: unique identifier for cell. center_x: cell center position along x axis in the original image, in pixels center_y: cell center position along y axis in the original image, in pixels x_pos.centered: center_x normalized to the position of the AP boundary for that disc. Positive numbers indicates posterior, negative indicates anterior. y_pos.centered: center_y normalized to the position of the DV boundary for that disc. Positive numbers indicates dorsal, negative indicates ventral. x_pos.elong.centered: center_x normalized to the position of the center of elongation symmetry. Positive numbers indicates posterior, negative indicates anterior. y_pos.elong.centered: center_y normalized to the position of the center of elongation symmetry. Positive numbers indicates dorsal, negative indicates ventral. area: cell area from tissue miner (uncorrected) area_tilt_factor: compensation due to tissue tilt corrected_area: height-corrected cell area Figure1_SourceData_TriangleElongation_EachMovie_AllTimes.csv Columns: movie: name of the movie. Ecd means it was acquired with 20nM 20hE, date of acquisition, F or P + # indicates the position (usually 2 discs were acquired each day, but only the one that didn't move or looked the brightest was taken). frame: frame # time_hr: actual elapsed time since start of movie, in hours (taken from the metadata of the microscope). tri_id: unique identifier for each triangle count: you can ignore this -- it was a check to make sure each triangle is associated with 3 cell_ids centroid_x: triangle centroid position along x axis in the original image, in pixels centroid_y: triangle centroid position along y axis in the original image, in pixels x_pos.centered: centroid_x normalized to the position of the AP boundary for that disc. Positive numbers indicates posterior, negative indicates anterior. y_pos.centered: centroid_y normalized to the position of the DV boundary for that disc. Positive numbers indicates dorsal, negative indicates ventral. x_pos.elong.centered: centroid_x normalized to the position of the center of elongation symmetry. Positive numbers indicates posterior, negative indicates anterior. y_pos.elong.centered: centroid_y normalized to the position of the center of elongation symmetry. Positive numbers indicates dorsal, negative indicates ventral. r: distance to the calculated elongation center Qt_xx: XX component of triangle elongation (already height-corrected) Qt_xy: XY component of triangle elongation (already height-corrected) Qt_RR: RR component of triangle elongation (already height-corrected) Qt_RPHI: RPHI component of triangle elongation (already height-corrected) corrected_area: triangle area (height-corrected) cell_id: unique identifier for associated cell. Note: each triangle has 3 (listed in count). I account for this repetition later when averaging. Figure1_SourceData_CenterPositions_EachMovie.csv Columns: movie: movie name x: x position of the calculated center of cell elongation symmetry, in pixels, in original image y: y position of the calculated center of cell elongation symmetry, in pixels, in original image ## References: ## Dye NA, Popovic M, Spannl S, Etournay R, Kainmueller D, Ghosh S, Myers G, Jülicher F, Eaton S. Cell dynamics underlying oriented growth of the Drosophila imaginal wing disc. Development. 2017. https://doi.org/10.1242/dev.155069 Dye NA, Popovic M, Iyer V, Eaton S, Jülicher F. Self-organized patterning of cell morphology via mechanosensitive feedback. BioRxiv. 2020. https://doi.org/10.1101/2020.04.16.044883 Etournay R, Merkel M, Popović M, Brandl H, Dye NA, Aigouy B, Salbreux G, Eaton S, Jülicher F. TissueMiner: A multiscale analysis toolkit to quantify how cellular processes create tissue dynamics. Elife. 2016. https://doi.org/10.7554/eLife.14334