Distance matrix of genes, including the 17 candidate genes that showed meaningful groupings in the previous PCA. rgs2 cfos ier2 klf4 anxa srl col6 tpm4 ryr tnnt thbs3 des col12 osx cfos 1.3406908 ier2 1.2241928 0.5207112 klf4 1.0485950 1.6135334 1.4015867 anxa 2.2132786 2.7492120 2.5288956 2.4770168 srl 2.4420559 3.0249532 2.8694260 2.6957855 1.4426654 col6 2.5943750 3.0709590 2.8871190 2.9707531 2.2917948 2.8876823 tpm4 2.9269302 3.3550846 3.2149631 3.0019186 1.7673035 1.3408735 3.2810650 ryr 2.4412039 2.9732476 2.7799360 2.7057355 1.1625975 1.3836415 2.5407481 1.4344779 tnnt 2.6199897 3.1035863 2.9303856 2.7902783 1.4256235 1.1761732 3.0207785 0.9137062 1.1285596 thbs3 2.4941580 2.9909548 2.8769100 2.9288387 2.2026894 2.5458231 2.0594390 3.0805121 2.3964437 2.9538675 des 2.4851559 3.0743130 2.9053055 2.6746326 1.3686923 1.3927915 2.8676948 1.3267958 1.2165099 1.0198657 2.6927931 col12 2.2705951 2.7753707 2.5942293 2.7396834 1.5214508 2.3269860 1.9476868 2.9521508 2.0854926 2.5847878 1.7624695 2.4872006 osx 2.0718498 2.5483337 2.3111000 2.3986543 1.3121450 2.0234577 2.0209767 2.5765064 1.8410486 2.2534706 1.9526698 2.0920978 0.9622262 runx2b 1.9874320 2.5653120 2.3631488 2.4105331 1.5625150 2.0797254 1.3253534 2.5952630 1.8227888 2.2819873 1.9696339 2.1244746 1.3325023 1.1196643 Distance matrix of the fish specimens using expression levels of the 17 candidate genes that showed meaningful groupings in the previous PCA. HD01_1mo HD02_1mo HD03_1mo HD04_1mo SD01_1mo SD02_1mo SD03_1mo SD04_1mo HD05_3mo HD06_3mo HD07_3mo HD08_3mo SD05_3mo SD06_3mo SD07_3mo SD08_3mo HD09_5mo HD10_5mo HD11_5mo HD12_5mo SD09_5mo SD10_5mo SD11_5mo SD12_5mo HD13_8mo HD14_8mo HD15_8mo HD16_8mo HD17_8mo HD18_8mo SD13_8mo SD14_8mo HD02_1mo 1.2928571 HD03_1mo 0.4214286 1.0071429 HD04_1mo 0.9964286 1.3750000 1.2392857 SD01_1mo 1.7178571 0.4750000 1.2714286 0.7892857 SD02_1mo 0.3642857 0.7535714 0.6642857 1.3464286 1.4428571 SD03_1mo 0.2142857 1.2750000 0.5107143 0.9535714 1.6357143 0.4642857 SD04_1mo 1.6392857 0.9107143 1.4250000 0.8178571 0.2750000 1.4750000 1.7321429 HD05_3mo 1.3714286 0.8928571 1.3107143 1.1678571 0.7571429 1.5000000 1.4785714 0.6571429 HD06_3mo 1.1857143 1.3928571 1.2571429 0.5964286 0.9107143 1.3678571 1.1571429 0.5464286 1.1607143 HD07_3mo 0.4214286 0.7142857 0.6571429 1.1428571 1.3571429 0.2250000 0.2964286 1.6464286 1.5178571 1.4714286 HD08_3mo 1.3785714 1.3928571 1.4285714 0.1928571 0.7714286 1.4964286 1.2500000 0.7000000 1.0571429 0.4714286 1.4214286 SD05_3mo 1.2071429 0.4000000 0.9964286 1.2464286 0.8035714 0.9607143 1.0821429 1.2642857 1.0214286 1.3392857 0.7857143 1.2642857 SD06_3mo 1.5428571 1.3892857 1.5750000 0.5500000 0.7142857 1.8464286 1.6000000 0.4321429 0.4785714 0.4928571 1.7678571 0.3535714 1.2607143 SD07_3mo 1.5250000 1.3142857 1.4071429 0.3642857 0.5892857 1.5892857 1.5107143 0.4428571 1.1000000 0.3285714 1.6142857 0.1285714 1.3178571 0.3000000 SD08_3mo 1.3321429 1.1250000 1.0964286 0.7714286 0.9071429 1.5571429 1.3642857 1.0571429 0.7357143 0.8821429 1.3571429 0.5821429 0.6500000 0.4678571 0.6571429 HD09_5mo 1.0321429 1.4000000 0.9392857 1.1642857 1.0428571 1.2785714 1.1571429 0.6392857 0.5250000 0.9392857 1.4035714 1.1857143 1.3035714 0.7285714 1.1321429 1.0214286 HD10_5mo 0.7285714 1.5964286 1.1714286 0.7714286 1.3535714 0.9428571 0.5285714 1.0714286 1.2071429 0.5892857 0.8357143 0.8964286 1.3750000 0.9250000 1.0035714 1.1964286 0.6392857 HD11_5mo 1.7357143 0.5857143 1.4392857 0.7535714 0.1035714 1.4821429 1.7678571 0.2642857 0.7428571 0.8178571 1.5000000 0.7035714 0.6714286 0.5892857 0.5285714 0.7142857 0.9642857 1.3321429 HD12_5mo 1.7250000 0.5321429 1.1464286 1.3357143 0.4500000 1.3714286 1.6107143 0.7285714 0.7214286 1.2857143 1.2035714 1.0535714 0.6642857 0.8107143 0.8750000 0.6071429 1.1071429 1.5357143 0.5178571 SD09_5mo 0.4107143 1.0678571 0.5892857 1.1785714 1.4892857 0.3392857 0.3321429 1.3178571 1.2571429 1.0107143 0.4535714 1.2357143 1.3428571 1.4964286 1.4000000 1.5250000 1.1071429 0.7500000 1.6892857 1.4750000 SD10_5mo 1.5000000 0.2535714 1.2464286 0.9750000 0.2357143 1.0714286 1.2678571 0.6857143 1.0857143 1.0142857 0.8464286 1.0357143 0.5785714 1.1250000 0.9000000 1.1357143 1.3857143 1.1821429 0.3964286 0.5785714 1.2785714 SD11_5mo 0.3285714 1.2821429 0.4714286 0.8642857 1.4107143 0.6142857 0.5642857 1.4107143 1.2357143 1.2785714 0.6642857 1.1821429 0.9357143 1.3642857 1.3571429 0.9607143 0.6785714 0.7928571 1.2892857 1.5250000 0.8607143 1.4392857 SD12_5mo 0.2821429 1.2964286 0.6035714 1.3571429 1.7892857 0.4285714 0.3071429 1.7785714 1.3607143 1.4750000 0.4178571 1.5392857 0.9357143 1.5785714 1.6607143 1.2178571 0.9857143 0.7357143 1.7392857 1.3178571 0.6642857 1.5000000 0.4857143 HD13_8mo 0.1607143 1.5035714 0.4214286 1.0785714 1.6464286 0.5500000 0.4178571 1.3285714 1.3142857 1.0035714 0.6750000 1.3785714 1.4928571 1.3642857 1.3714286 1.3642857 0.6285714 0.5714286 1.6571429 1.6107143 0.5178571 1.5964286 0.3678571 0.3785714 HD14_8mo 1.7642857 1.0642857 1.7071429 0.8321429 0.4714286 1.6714286 1.7071429 0.3535714 0.6071429 0.5642857 1.7535714 0.5178571 1.1571429 0.2571429 0.3214286 0.7464286 1.0000000 1.0642857 0.4178571 0.5892857 1.5357143 0.7500000 1.7178571 1.6285714 1.6285714 HD15_8mo 0.6714286 1.4964286 0.6785714 1.0000000 1.3392857 0.8464286 0.4035714 1.1178571 1.4535714 0.7428571 0.7464286 1.0607143 1.5250000 1.2000000 1.0250000 1.5000000 0.8428571 0.3821429 1.5642857 1.3392857 0.4535714 1.1821429 0.9821429 0.6678571 0.4750000 1.2178571 HD16_8mo 1.5285714 1.5178571 1.4857143 0.4642857 0.7642857 1.7678571 1.5178571 0.5750000 0.7750000 0.5285714 1.7642857 0.1928571 1.2750000 0.1428571 0.1500000 0.4928571 0.8571429 0.9321429 0.6357143 0.8714286 1.5571429 1.1607143 1.2392857 1.4750000 1.3678571 0.2714286 1.1071429 HD17_8mo 0.9964286 1.7178571 1.2857143 0.2750000 1.1678571 1.5250000 0.9071429 0.9178571 1.0071429 0.3964286 1.4607143 0.2714286 1.3500000 0.3964286 0.4107143 0.7392857 0.9535714 0.6535714 1.0357143 1.4321429 1.1428571 1.3857143 1.0392857 1.1750000 0.9857143 0.6857143 0.8392857 0.3250000 HD18_8mo 0.1964286 1.4035714 0.2928571 1.0392857 1.5750000 0.4928571 0.3500000 1.3821429 1.5964286 0.9250000 0.6250000 1.2821429 1.4464286 1.5107143 1.2321429 1.4392857 0.9500000 0.7357143 1.6607143 1.5678571 0.4392857 1.4535714 0.5142857 0.4428571 0.1071429 1.6250000 0.3714286 1.3857143 0.9642857 SD13_8mo 0.2714286 1.3250000 0.6250000 1.1821429 1.8678571 0.4500000 0.1142857 1.8250000 1.4000000 1.1642857 0.3750000 1.2821429 1.1321429 1.5357143 1.5178571 1.3071429 1.1642857 0.5928571 1.9392857 1.5392857 0.3357143 1.4500000 0.7000000 0.2321429 0.4357143 1.6392857 0.4607143 1.4535714 0.9321429 0.3892857 SD14_8mo 1.3035714 0.1535714 0.9500000 1.5535714 0.6642857 0.7607143 1.3035714 1.1000000 0.8071429 1.6821429 0.7035714 1.4571429 0.3821429 1.3785714 1.4250000 0.8964286 1.2678571 1.6428571 0.7250000 0.3071429 1.1607143 0.5250000 1.1571429 1.0285714 1.4500000 1.1107143 1.5464286 1.4500000 1.8214286 1.4357143 1.2500000 SD15_8mo 0.3964286 1.4428571 0.4821429 1.1071429 1.6250000 0.6392857 0.2571429 1.4250000 1.4250000 0.8285714 0.6428571 1.2107143 1.1785714 1.3607143 1.3178571 1.1785714 0.9392857 0.5857143 1.6535714 1.4214286 0.3285714 1.5035714 0.7250000 0.4250000 0.3821429 1.5142857 0.3571429 1.3750000 0.8250000 0.3107143 0.3035714 1.4071429 Distance matrix of genes, including all 19 candidate genes . rgs2 cfos c1ql abcb3 alas1 ier2 klf4 gif anxa srl col6 tpm4 ryr tnnt thbs3 des col12 cfos 1.3406908 c1ql 2.2473025 2.9699751 abcb3 2.8323422 3.3186680 3.0584687 alas1 1.8038599 2.3077821 2.0094261 2.7393018 ier2 1.2241928 0.5207112 2.7220307 3.1853126 2.0690991 klf4 1.0485950 1.6135334 2.5170077 2.9296147 1.9450606 1.4015867 gif 2.3041835 2.8259935 1.9275090 3.0462278 1.5374397 2.6622896 2.3852174 anxa 2.2132786 2.7492120 1.7032452 3.0897031 2.0058235 2.5288956 2.4770168 2.2039454 srl 2.4420559 3.0249532 2.0284934 2.9366659 2.2544642 2.8694260 2.6957855 2.6478506 1.4426654 col6 2.5943750 3.0709590 2.5518221 3.2438174 1.8696312 2.8871190 2.9707531 2.3582536 2.2917948 2.8876823 tpm4 2.9269302 3.3550846 2.6432753 3.6723159 2.8418872 3.2149631 3.0019186 3.1127011 1.7673035 1.3408735 3.2810650 ryr 2.4412039 2.9732476 2.3436428 3.1247711 2.2626558 2.7799360 2.7057355 2.6324970 1.1625975 1.3836415 2.5407481 1.4344779 tnnt 2.6199897 3.1035863 2.2596429 3.4703825 2.5531258 2.9303856 2.7902783 2.8021030 1.4256235 1.1761732 3.0207785 0.9137062 1.1285596 thbs3 2.4941580 2.9909548 2.8748687 3.1325620 2.3588801 2.8769100 2.9288387 2.9729587 2.2026894 2.5458231 2.0594390 3.0805121 2.3964437 2.9538675 des 2.4851559 3.0743130 2.2531586 3.3069305 2.4816863 2.9053055 2.6746326 2.6601609 1.3686923 1.3927915 2.8676948 1.3267958 1.2165099 1.0198657 2.6927931 col12 2.2705951 2.7753707 1.9658102 2.9815624 1.9697908 2.5942293 2.7396834 2.2968649 1.5214508 2.3269860 1.9476868 2.9521508 2.0854926 2.5847878 1.7624695 2.4872006 osx 2.0718498 2.5483337 1.7558955 2.7738533 1.6352188 2.3111000 2.3986543 2.1271788 1.3121450 2.0234577 2.0209767 2.5765064 1.8410486 2.2534706 1.9526698 2.0920978 0.9622262 Distance matrix of fish specimens using expression levels of all 19 candidate genes. HD01_1mo HD02_1mo HD03_1mo HD04_1mo SD01_1mo SD02_1mo SD03_1mo SD04_1mo HD05_3mo HD06_3mo HD07_3mo HD08_3mo SD05_3mo SD06_3mo SD07_3mo SD08_3mo HD09_5mo HD10_5mo HD11_5mo HD12_5mo SD09_5mo SD10_5mo SD11_5mo SD12_5mo HD13_8mo HD14_8mo HD15_8mo HD16_8mo HD17_8mo HD18_8mo SD13_8mo SD14_8mo HD02_1mo 1.1228070 HD03_1mo 0.4994840 1.0939112 HD04_1mo 0.9865841 1.1475748 0.9184727 SD01_1mo 1.7647059 0.6150671 1.3003096 0.9865841 SD02_1mo 0.4437564 0.5614035 0.9948400 1.1640867 1.3622291 SD03_1mo 0.2125903 1.1021672 0.5036120 0.6418989 1.6800826 0.6130031 SD04_1mo 1.5810114 0.8937049 1.4716202 0.8441692 0.4643963 1.0753354 1.5748194 HD05_3mo 1.2549020 0.7492260 1.3601651 1.0670795 0.8751290 1.0939112 1.3353973 0.5675955 HD06_3mo 1.1950464 1.2755418 1.4097007 0.9989680 0.8462332 1.1517028 1.2260062 0.6006192 1.0918473 HD07_3mo 0.5263158 0.7966976 0.7987616 1.0691434 1.3684211 0.3942208 0.3921569 1.4138287 1.2301342 1.3684211 HD08_3mo 1.3808050 1.4179567 1.2404541 0.6439628 0.6728586 1.5087719 1.2776058 0.9618163 1.3023736 0.5799794 1.3704850 SD05_3mo 1.1537668 0.9556244 0.7306502 1.1950464 0.8070175 1.3973168 1.1166151 1.4654283 1.3188854 1.3704850 1.0464396 0.8524252 SD06_3mo 1.4613003 1.3519092 1.3704850 0.8916409 0.6398349 1.7502580 1.4551084 0.7719298 0.7863777 0.5325077 1.6140351 0.3488132 0.8916409 SD07_3mo 1.5190918 1.4757482 1.1001032 0.5841073 0.5985552 1.5892673 1.3890609 0.7182663 1.3519092 0.6047472 1.5397317 0.1630547 0.8998968 0.4891641 SD08_3mo 1.2445820 1.2094943 0.8627451 0.9081527 0.8524252 1.6615067 1.2136223 1.3085655 1.0794634 1.0175439 1.3209494 0.4953560 0.3921569 0.3777090 0.6315789 HD09_5mo 0.9349845 1.3849329 0.8833849 1.2218782 1.0196078 1.2528380 1.2301342 0.9576883 0.9391125 0.9680083 1.4427245 1.0257998 0.8833849 0.8132095 0.8833849 0.9514964 HD10_5mo 0.6790506 1.2321981 1.3746130 1.1785346 1.2260062 0.7512900 0.8049536 1.0712074 1.1289990 0.3921569 0.8875129 0.9721362 1.3684211 0.9019608 1.1393189 1.2693498 0.7430341 HD11_5mo 1.7296182 0.7905057 1.2899897 1.0650155 0.1424149 1.4427245 1.7874097 0.5696594 0.9680083 0.9143447 1.5748194 0.6398349 0.5531476 0.6191950 0.5159959 0.6687307 0.7450980 1.2610939 HD12_5mo 1.5851393 0.6377709 1.2755418 1.5500516 0.4313725 1.2239422 1.6615067 0.8875129 0.9432405 0.9535604 1.1517028 0.9019608 0.7822497 0.7120743 0.9308566 0.6811146 1.0877193 1.1372549 0.4912281 SD09_5mo 0.3859649 0.8235294 0.9102167 1.2177503 1.3415893 0.4066047 0.5448916 1.2734778 1.1785346 0.7389061 0.6357069 1.1207430 1.3395253 1.1661507 1.4055728 1.3209494 1.0815273 0.4726522 1.5170279 1.1248710 SD10_5mo 1.3787410 0.2579979 1.3890609 1.0464396 0.3611971 0.8028896 1.2425181 0.6955624 0.8978328 0.8194014 0.8875129 1.1042312 1.1145511 1.1001032 1.1207430 1.2507740 1.3395253 0.8379773 0.6377709 0.5531476 0.9432405 SD11_5mo 0.4396285 1.0361197 0.5490196 0.7492260 1.4262126 0.7100103 0.4747162 1.2074303 0.9205366 1.3106295 0.5902993 1.3539732 1.0546956 1.3085655 1.4220846 0.9989680 1.0257998 1.0402477 1.4303406 1.5190918 0.9618163 1.2404541 SD12_5mo 0.3859649 0.8813209 0.8998968 1.0196078 1.6904025 0.3261094 0.4024768 1.2631579 1.0526316 1.2590299 0.4107327 1.6635707 1.3642931 1.6594427 1.6449948 1.4509804 1.3044376 0.7966976 1.7048504 1.3250774 0.7698658 1.0485036 0.5036120 HD13_8mo 0.5944272 1.5830753 0.5159959 0.9803922 1.5170279 0.9287926 0.6398349 1.0856553 1.0732714 1.2280702 0.6955624 1.3085655 1.1517028 1.3849329 1.0939112 1.2817337 0.7141383 1.0381837 1.4613003 1.5232198 1.1166151 1.6202270 0.5964912 0.6976264 HD14_8mo 1.7874097 1.1640867 1.4427245 0.8132095 0.5345717 1.5438596 1.5603715 0.4107327 0.6336429 0.8462332 1.4736842 0.7100103 1.0423117 0.5902993 0.4912281 0.8916409 1.1310630 1.3292054 0.5634675 0.8194014 1.6656347 0.9473684 1.4695562 1.4241486 1.0650155 HD15_8mo 0.6501548 1.2507740 0.7801858 1.1186791 1.2033024 0.9184727 0.5283798 1.2693498 1.4716202 0.6439628 0.7141383 0.9721362 1.1991744 0.9638803 1.0464396 1.2094943 0.9927761 0.4582043 1.4427245 1.0732714 0.4189886 0.9783282 0.9865841 0.8833849 0.8854489 1.3704850 HD16_8mo 1.4901961 1.6284830 1.1228070 0.7925697 0.7017544 1.7688338 1.4509804 0.9184727 1.2033024 0.7595459 1.5892673 0.1857585 0.6831785 0.3364293 0.1176471 0.4478844 0.7058824 1.1372549 0.5180599 0.8957688 1.5046440 1.3374613 1.3828689 1.7007224 1.0278638 0.4767802 1.1062951 HD17_8mo 1.0134159 1.7048504 1.1001032 0.5077399 1.1104231 1.5211558 0.9060888 0.9948400 1.2528380 0.4602683 1.4530444 0.3240454 1.0712074 0.4602683 0.3715170 0.7182663 0.8978328 0.7595459 1.0113519 1.3704850 1.1001032 1.3704850 1.1413829 1.2899897 0.9556244 0.7966976 0.8276574 0.3715170 HD18_8mo 0.4066047 1.3065015 0.5923633 0.9391125 1.5933953 0.5428277 0.4437564 1.0340557 1.1805986 0.9122807 0.5655315 1.3849329 1.5665635 1.5397317 1.2487100 1.5541796 1.1062951 0.7946336 1.7254902 1.5108359 0.6976264 1.2858617 0.5428277 0.3818369 0.2765738 1.3168215 0.6006192 1.3993808 0.9556244 SD13_8mo 0.2951496 1.2549020 0.7801858 1.3106295 1.7502580 0.6088751 0.3570691 1.7481940 1.4097007 1.0216718 0.4561404 1.1496388 1.0072239 1.3436533 1.3911249 1.1826625 1.0691434 0.5428277 1.7316821 1.2693498 0.3839009 1.3312693 0.8998968 0.5283798 0.7182663 1.6037152 0.4623323 1.2817337 0.9391125 0.5655315 SD14_8mo 1.3477812 0.3529412 0.9824561 1.4138287 0.6191950 0.8751290 1.4138287 1.0299278 0.8132095 1.6119711 0.8441692 1.3065015 0.5902993 1.3106295 1.2528380 0.9287926 1.0897833 1.5459236 0.5861713 0.3983488 1.2631579 0.6625387 1.2053664 1.0939112 1.2321981 0.9349845 1.5170279 1.2053664 1.7853457 1.4447884 1.2734778 SD15_8mo 0.5758514 1.3477812 0.4272446 0.8689370 1.5500516 0.8565531 0.3942208 1.2342621 1.3353973 1.1351909 0.5799794 1.2899897 0.9618163 1.4633643 1.1310630 1.1578947 1.1042312 1.1269350 1.5232198 1.5067079 0.9226006 1.4984520 0.7120743 0.6460268 0.2641899 1.1475748 0.7946336 1.2053664 0.9122807 0.3529412 0.5861713 1.2280702 >