README file accompanying Supplementary figures e1-e3 and Appendix e-1 for Ò[11C]PK11195-PET brain imaging of the mitochondrial translocator protein in mitochondrial diseaseÓ Figure e-1. Age and gender distribution of patients and controls. A. Number of patients and controls according to age (Mann-Whitney U test). B. Number of female (light) and male (dark) controls (green) and controls (blue) (FisherÕs exact test). Figure e-2. Individual regional [11C]PK11195 voxel fractions with significant change in binding potential. A,B. Fraction of each grey (A) or white (B) matter ROI for each individual control (green), patient with mitochondrial disease (blue) or asymptomatic carrier (red), where BPND was significantly (p<0.001) increased (top) or decreased (bottom) compared to the control population. Figure e-3. Clinical severity is associated with changes in [11C]PK11195 binding potential (BPND). A-D. Violin plots of all ROIs across the brain showing the fraction of significantly (p<0.001) increased (top) or decreased (bottom) BPND, for each patient with MT-TL1 (A), MT-ND6 (B), POLG (C) or SPG7 (D) mutations. E-P. Overlay of -log10(p-value) for voxels with significantly increased (E,G,J,L,N) or decreased (F,H,K,M,O) BPND (p<0.05; p²0.001 in red) on the ICBM 152 2009a T1 MRI template at regular dorso-ventral intervals across the brain (E-H) or in the cerebellum (J-O), and correlation between score on the modified Rankin Scale (mRS) and the fraction of the brain (I) or the Scale for the Assessment and Rating of Ataxia (SARA) and cerebellum (P) where binding potential (BPND) was significantly (p<0.001) different from the control population, for each patient with MT-ND6 m.14487T>C (E-I) or SPG7 mutations (J-P). Appendix e-1. All patient -log10(p) maps are displayed as overlay of -log10(p-value) for voxels with significantly increased (top) or decreased (bottom) binding potential (p<0.05; p²0.001 in red) on the ICBM 152 2009a T1 MRI template for each patient compared to controls. Scale shown for Patient 1 applies for all other images. All images are shown in radiological format (i.e. left of the brain is shown on the right of the image and vice versa).