The folders for Sox19b and TBP contain the following items: i) SPT_..._ITM: a list of all tracks observed, sorted by stage and embryo. The position in pixels inx y is given for each spot belonging to the track. ii) SPT_..._ITM_events: number of tracks classified as stable binding, short binding or free is given for every stage and every embryo.This is the basis for the subsequent ITM analysis. iii) .._nuclear_areas: Here, the average cross-sectional area per embryo and stage is listed. From these Values the nuclear volume is calculated. Additionally the folder for TBP contains a file SPT_TBP_ITM_events_ff. It contains the Average number of tracks found in the first frame after phyotactivation an the average cross-sectional area per embryo and stage