This PersonalRiskSocialSafetyReadMe.txt file was generated on 2021-07-23 by Laurence Feyten GENERAL INFORMATION 1. Title of Dataset: Predation risk shapes the use of conflicting personal risk and social safety information in guppies 2. Author Information A. Principal Investigator Contact Information Name: Laurence E A Feyten Institution: Concordia University Address: Montreal, QC, CAN Email: laurencefeyten@mac.com B. Associate or Co-investigator Contact Information Name: Grant E Brown Institution: Concordia University Address: Montreal, QC, CAN Email: grant.brown@concodia.ca 3. Date of data collection: 2019-03-22 to 2019-03-26 4. Geographic location of data collection: Lopinot and Upper Aripo Rivers in the Northern Range Mountains of Trinidad and Tobago. 5. Information about funding sources that supported the collection of the data: This work was supported by funding to GEB by the Natural Science and Engineering Research Council of Canada, Discovery Grants. SHARING/ACCESS INFORMATION 1. This data corresponds to: Feyten, LEA, Crane, AL, Ramnarine, IW, Brown, GE. 2021. Predation risk shapes the use of conflicting personal risk and social safety information in guppies, Behavioral Ecology. 2. Recommended citation for this dataset: Feyten, LEA, Crane, AL, Ramnarine, IW, Brown, GE. 2021. Data from: Predation risk shapes the use of conflicting personal risk and social safety information in guppies, Behavioral Ecology. https://doi.org/10.5061/dryad.kwh70rz44. DATA & FILE OVERVIEW 1. File List: ÒExperiment 1.csvÓ contains the data collected for Experiment 1 (described below), with 3 independent variables (population, chemical cue treatment, and shoal treatment) and 2 response variables (latency to enter and latency to forage in a novel foraging arena). ÒExperiment 2.csvÓ contains the data collected for Experiment 2 (described below), with 6 independent variables (population, and standard lengths, average standard length, and variance of standard length) and 2 response variables (number of calm crosses and number of dashing crosses). 2. Relationship between files: Experiment 2 was conducted to determine whether the stimulus shoals used in Experiment 1 may have behaved differently depending on population. METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: In March 2019, we conducted observations of guppies in the Lopinot and Upper Aripo Rivers in the Northern Range Mountains of Trinidad. For Experiment 1, we conducted field observations by gently placing a novel foraging arena (corrugated plastic; ~30x25x23 cm) into stream pools at locations that visibly contained at least 5 guppies in locations without visible currents or higher velocities. For half of the trials, the social safety cue was present, consisting of 3 female guppies inside a clear 1-L chamber (~7x7x24 cm) filled with stream water and gravel (~3 cm depth).ÊFor the other half of trials, the chamber was filled with water and gravel but did not contain guppies. We began our trials by gently injecting 10 mL of one of the three personal cue treatments (alarm cue, novel cue, or stream water) through airline tubing on the back wall of the arena before flushing the tubing with 60 mL of stream water and beginning a 5-min (300s) observation period. We recorded the latency for guppies to enter the foraging zone (crossing from the approach zone into the foraging zone), as well as the latency to forage inside the foraging zone. These two latencies could correspond to two different guppies. After each trial, we moved at least 1 m upstream before initiating the next trial, which ensured we avoided re-testing guppies or testing downstream guppies potentially exposed to the experimental cues from previous trials. The cue treatments were randomly assigned, and we conducted a total of 90 trials per population (180 total), with a sample size of 15 per treatment group.Ê For Experiment 2, we conducted a behavioral assay to determine whether the movement activity of our experimental shoals differed between the high- and low-predation streams (30 trials from each population). From each stream, we collected 90 female guppies in total, moving 3 individuals at a time into 1-L chambers that were positioned on a flat surface along the stream bank. The chamber again contained stream water (0.75 L) and gravel, was surrounded by opaque walls on 3 sides to minimize external visual stimuli, and was demarcated into 4 quadrants by exterior lines. After a 1-min acclimation period, we tallied the total number of lines crossed by all 3 guppies over a 1-min observation period. We also recorded whether the line crosses were dashes (rapid bursts of movement with the entire body crossing the line in under 0.5 s) or were calmer movements that are typical of normal swimming behavior (> 0.5 s). After each trial, we recorded the standard length (head to caudal peduncle) of each fish and released them at their site of capture.Ê More detailed methodology for data collection can be found in Feyten et al. 2021, Behavioral Ecology. 2. Methods for processing the data: The data were recorded on data sheets in the field, and transcribed into Excel sheets. The data was not processed further. 3. Software-specific information needed to interpret the data: The authors used RStudio 3.4.1 to analyze their data, using alpha = 0.05. Libraries used for Experiment 1 analyses include car, ARTool, emmeans, lsmeans, ggplot2, grid, gridExtra, ggsignif, and forcats. Libraries used for Experiment 2 analyses include car, vegan, rcompanion, ggplot2, ggsignif, grid, and gridExtra 4. People involved with sample collection, processing, analysis and/or submission: Data collection by Laurence E.A. Feyten, Adam L. Crane, Grant E. Brown. Data processing and analysis by Laurence E.A. Feyten and Grant E. Brown. DATA-SPECIFIC INFORMATION FOR: [Experiment 1.csv] 1. Number of variables: 5 2. Number of cases/rows: 180 3. Variable List: ÒPOPÓ is an independent variable, the source population, where ÒUAÓ= Upper Aripo, and ÒLOPÓ= Lopinot ÒSTIMÓ is an independent variable, the chemical cue treatment, where ÒSWÓ= stream water control, ÒACÓ= alarm cue, and ÒLOÓ= lemon odour (a novel cue) ÒSHOALÓ is an independent variable indicating whether a stimulus shoal was present, ÒYÕ= present, ÒNÓ= absent ÒLAT EÓ is corresponds to a response variable, the latency to enter the novel arena (seconds) ÒLAT FÓ is corresponds to a response variable, the latency to forage in the novel arena (seconds) DATA-SPECIFIC INFORMATION FOR: [Experiment 2.csv] 1. Number of variables: 8 2. Number of cases/rows: 60 3. Variable List: ÒPOPÓ is an independent variable, the source population, where ÒUAÓ= Upper Aripo, and ÒLOPÓ= Lopinot ÒCALMÓ is a response variable, the tally of the number of calm crosses (normal swimming behaviour with the entire body crossing the line in >0.5s) during a trial ÒDASHINGÓ is a response variable, a tally of the number of dashing crosses (rapid bursts of movement with the entire body crossing the line in under 0.5 s) during a trial ÒSL_1Ó, ÒSL_2Ó, and ÒSL_3Ó are independent variables, corresponding the standard lengths (mm) of the 3 guppies used in the shoal assay. ÒAV_SLÓ is the calculated average standard length (mm) of guppies used in the shoal assay ÒVAR_SLÓ is the calculated variance of standard lengths of guppies used in the shoal assay 4. Special Case: In the case of 0 total movement (no crosses made), the ratio calculations result in NaN. In such a scenario, we set the ratio NaN value to 0 (if a shoal made no crosses, their proportion of calm crosses were effectively 0). This is seen in row 11 (excluding header) of the data.