Spatial Reconstruction of the Early Hepatic Transcriptomic Landscape after an Acetaminophen Overdose using Single-Cell RNA Sequencing DOI: 10.1093/toxsci/kfab052 Author(s): David S Umbaugh, Anup Ramachandran, Hartmut Jaeschke Date Created: 2021-08-01 Data For more details, refer to METHODS in manuscript. Additional File 1: Table indicating number of hepatocytes, endothelial and Kupffer cells identified within either the control or APAP dataset. Additional File 2 and 3: Column names are the Gene name, mean square error, slope, standard error, p-value, adjusted p-value, zonation pattern. Additional File 4: Pearson correlation tables Additional File 5, 6, and 7: Seurat wilcoxon rank sum test performed. Column 1 is gene names. In respective order, p-value (unadjusted), average log fold change, percent of cells in the respective group expressing the gene (pct.1), percent of cells in the respective group expressing the gene (pct.2), adjusted p value. Additional File 8: First column are gene names. Other columns are either control or acetaminophen groups divided into 9 layers, where layer 1 represents the most pericentral hepatocytes, and layer 9 represent the most periportal hepatocytes. Values were obtained by Using the AverageExpression() function in Seurat giving gene expression for an ‘average’ cell among the 18 identity classes (e.g. layer 1 APAP, layer 1 control, layer 2 APAP, layer 2 control etc.) Additional File 9: Same format as additional files 5,6,7. Differential expression using Wilcoxon rank sum test in Seurat comparing the pericentral clusters that were predominately APAP hepatocytes. Additional File 10: Same format as additiona files, 5, 6,7, 9. Differential expression using Wilcoxon rank sum test in Seurat comparing the Glul+/StressHigh subclusters. Additional File 11: Same format as additional files 2 and 3. List of all sampled genes from human hepatocytes (GSE124395), ordered based on mean square error, assigned either as perivenous (PV, e.g. pericentral), periportal (PP) or no zonation. Additional File 10: