This Electrophysiological recordings and a liposome-based assay showing the absence of chloride and proton conduction in TTYH proteins_Readme.txt file was generated on 2021-07-21 by Anastasiia Sukalskaia GENERAL INFORMATION 1. Title of Dataset: Electrophysiological recordings and a liposome-based assay showing the absence of chloride and proton conduction in TTYH proteins 2. Author Information A. Principal Investigator Contact Information Name: Raimund Dutzler Institution: University of Zurich Address: Winterthurerstrasse 190, Zurich 8057, Switzerland Email: dytzler@bioc.uzh.ch B. Alternate Contact Information Name: Anastasiia Sukalskaia Institution: University of Zurich Address: Winterthurerstrasse 190, Zurich 8057, Switzerland Email: a.sukalskaia@bioc.uzh.ch 3. Date of data collection (single date, range, approximate date) October 2020 - June 2021 4. Geographic location of data collection : Winterthurerstrasse 190, Zurich 8057, Switzerland SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: no 2. Links to publications that cite or use the data: publication in progress DATA & FILE OVERVIEW 1. File List: TTYH_absence_of_chloride_conduction.xlsx - data from electrophysiological recordings showing the absence of chloride conduction in TTYH proteins; TTYH_absence_of_proton_conduction.xlsx - data from electrophysiological recordings and a liposome-based assay showing the absence of proton consuction in TTYH proteins. Electrophysiological recordings showing the absence of chloride and proton conduction in TTYH proteins.zip - raw data from electrophysiological recordings showing the absence of chloride and proton conduction in TTYH proteins The zip file contains the following folders: - HEK293_chloride_currents; - TTYH1_Calcium_chloride_currents; - TTYH1_Calcium_proton_currents; - TTYH1_chloride_currents; - TTYH2_Calcium_chloride_currents; - TTYH2_Calcium_proton_currents; - TTYH2_chloride_currents; - TTYH3_Calcium_chloride_currents; - TTYH3_Calcium_proton_currents; - TTYH3_chloride_currents; - VRAC--_AC_chloride_currents; - VRAC--_AQP4_chloride_currents; - VRAC--_AQP4_TTYH1_chloride_currents; - VRAC--_Calcium_chloride_currents; - VRAC--_Calcium_proton_currents; - VRAC--_chloride_currents. All files with raw electrophysiological data are ABF files and can be open with Clampfit software. 2. Are there multiple versions of the dataset? no A. If yes, name of file(s) that was updated: i. Why was the file updated? ii. When was the file updated? METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: TTYH_absence_of_chloride_conduction.xlsx: whole-cell elextrophysiological recordings TTYH_absence_of_proton_conduction.xlsx: whole-cell electrophysiological recordings and a liposome-based assay for detection of proton transport in membrane proteins Lee, S. Y., Letts, J. A. & MacKinnon, R. Functional reconstitution of purified human Hv1 H+ channels. J Mol Biol 387, 1055-1060, doi:10.1016/j.jmb.2009.02.034 (2009). 2. Methods for processing the data: All electrophysiological data were analyzed in Clampfit 10.7 (Molecular Devices).