Metadata for all datafiles from: Piper C, Siciliano S, Winsley T, Lamb E Data from: Smooth brome invasion increases rare soil bacterial species prevalence and alters soil bacterial community composition. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.5sn8m Each section below describes the variables included in each datafile. File Piper_plotdata This file includes all aboveground plant community measurements. Variable Description Plot Field plot ID number; Numbers are 1-80 but not continuous as some locations were skipped due to unsuitability (i.e. no brome patches, in too high of shrubs, too close to wetland, etc. Brome_Cover_Class Plots were assigned to one of four smooth brome cover classes, 1- 0% brome, 2- >0-50%, 3- 51-85% and 4- >85% Plant_Rich Plant species richness in 50cm by 50cm quadrats Plant_Evar Plant community evenness, calculated using Evar Brome_gm2 Biomass of smooth brome shoots (g/m2) Root_gm2 Root biomass (g/m2) LitterCN_ratio Carbon:Nitrogen ratio of Litter (no units) A_Horizon Depth of the A horizon (cm) Grass_gm2 Biomass of grass shoots (g/m2); includes smooth brome Shrub_gm2 Biomass of shrub shoots (g/m2) Forb_gm2 Biomass of forb shoots (g/m2) Litter_gm2 Biomass of litter (g/m2) Tot_Biomass_gm2 Total aboveground live biomass (including all living shoots, not litter) (g/m2) gm2_Nitrogen Total N added to the soil via the litter (e.g. total amount of N in litter * amount of litter in plot) (g m2) File Piper_soilsdata This file includes all soil variables measured in both the A and B soil horizons at each plot. Variable Plot Field plot ID number; Numbers are 1-80 but not continuous as some locations were skipped due to unsuitability (i.e. no brome patches, in too high of shrubs, too close to wetland, etc. Horizon Indicates A or B horizon. Each horizon was sampled at each plot. Microbe_Rich Microbial species (UTU) richness Evar_microbe_n5 Microbial community (OTU) evenness (calculated with Evar) arch_amoA Log amoA copy number/ng DNA/g dry soil Standard_curve For the bacterial amoA data, copy numbers were low, and so were given a number based on whether they were within the standard curve, below it, or not detected--> 1-Samples detected but below standard curve, 2- Samples detected and within standard curve, 3- samples not detected and converted to lowest theoretical copy number for inclusion in model bacterial_amoA Log amoA copy number/ng DNA/g dry soil Grav_Water Gravimetric water content of soil (%) Org_C Soil organic carbon (%) Soil_TotN Total soil N (%) Total_C Total soil carbon (%) Microbe_Comp Microbial Community composition (ordination axis) pH soil pH UFW weighted uniFrac UFUW unweighted uniFrac File Piper_plantcommunity This file includes estimates of percent cover for all vascular plant species encountered in the plots. Variable Description Plot Field plot ID number; Numbers are 1-80 but not continuous as some locations were skipped due to unsuitability (i.e. no brome patches, in too high of shrubs, too close to wetland, etc. Genus_species Genus and species names for each taxa encountered. Nomemclature follows ITIS File Piper_bacterialOTU_taxonomy This file summarizes the taxonomy of bacterial OTUs observed in this study Taxonomic assignment of OTUs was achieved by BLAST comparison of the seed sequences against the Greengenes 2011 database (McDonald et al., 2012) by trimming the Greengenes sequences to the same region as the seed sequences to improve assignment (Werner et al., 2012). Numbers in brackets indicate the confidence in which a particular name was assigned e.g. (100) is 100% confidence that the correct name was assigned Some OTUs are unnamed at some level of classification (e.g. f__(100) indicates that an OTU belongs to a certain unnamed family). Alternatively, an OTU may be "unclassified" denoting that the species could not be fit with any known bacterial groups. Variable Description OTU OTU numbers as used in the OTUby sample matrix Kingdom Naming at each level of the taxonomic hierarchy Phylum Class Order Family Genus Species File Piper_bacterial_OTU_sample_matrix This file contains the raw OTU by sample matrix for all soil samples. OTU_names are explained in the file "Piper_bacterial_OTU_taxonomy The samples were analysed as a subsampled dataset in which a total of 114 samples were analysed, standardised to 2550 reads each (singleton OTUs were removed from the dataset, therefore the abundance totals don't all add up to 2550) This is a matrix of each OTU at species level (0.03 dissimilarity) and its standardised (to 2550 reads) occurrence in each sample. Variable Description Plot Field plot ID number; Numbers are 1-80 but not continuous as some samples did not yield sufficent reads for analysis Horizon Soil horizon OTU OTU numbers as described in the OTU_taxonomy file File Piper_Ahorizon_bacteria_phylum This file includes abundance estimates for bacterial OTUs in A-horizon soil samples clustered at the phylum level. Taxonomic assignment according to the Greengenes 2011 taxonomy Variable Description Plot Field plot ID number; Numbers are 1-80 but not continuous as some samples did not yield sufficent reads for analysis Names Taxonomy following greengenes 2011. Units are the sum of copy numbers in that taxon from the OTU by sample matrix. File Piper_Ahorizon_bacteria_order This file includes abundance estimates for bacterial OTUs in A-horizon soil samples clustered at the order level. Taxonomic assignment according to the Greengenes 2011 taxonomy Variable Description Plot Field plot ID number; Numbers are 1-80 but not continuous as some samples did not yield sufficent reads for analysis Names Taxonomy following greengenes 2011. Units are the sum of copy numbers in that taxon from the OTU by sample matrix. File Piper_Bhorizon_bacteria_phylum This file includes abundance estimates for bacterial OTUs in B-horizon soil samples clustered at the phylum level. Taxonomic assignment according to the Greengenes 2011 taxonomy Variable Description Plot Field plot ID number; Numbers are 1-80 but not continuous as some samples did not yield sufficent reads for analysis Names Taxonomy following greengenes 2011. Units are the sum of copy numbers in that taxon from the OTU by sample matrix. File Piper_Bhorizon_bacteria_order This file includes abundance estimates for bacterial OTUs in B-horizon soil samples clustered at the order level. Taxonomic assignment according to the Greengenes 2011 taxonomy Variable Description Plot Field plot ID number; Numbers are 1-80 but not continuous as some samples did not yield sufficent reads for analysis Names Taxonomy following greengenes 2011. Units are the sum of copy numbers in that taxon from the OTU by sample matrix.