Read me file of Supplementary material S1.xlsx Short description We applied two NGS technologies (454 pyrosequencing and Illumina sequencing) to characterize the transcriptome of a plant species of ecological and conservation relevance for which no genomic resource is available, Scabiosa columbaria L. We obtained 528,557 reads from a 454 GS-FLX run and a total of 28,993,627 reads from two lanes of an Illumina GAII single run. After read trimming, the de novo assembly of both types of reads produced 109,630 contigs. Both the contigs and the >75 bp remaining singletons were blasted against the Uniprot/Swissprot database using the software BLAST2GO, resulting in 29,676 and 10,515 significant hits, respectively. This file contains the full list of annotated contigs (sheet 1) and singletons >75bp (sheet 2). column headings Sequence: the nucleotide sequence of the contig (or singleton). Seq. Description: describes the matching sequence found in the uniprot/swissprot database with the lowest e-value. Seq. Length: reports the lenght of the sequence (number of nucleotides). #Hits: the number of matching sequences found in the uniprot/swissprot database. min. eValue: reports the lowest e-value found among all the matching sequences in the uniprot/swissprot database. #GOs:Reports the amount of Gene Ontology (GO) slim terms found for the query sequence. GOs: describes all the GO slim terms found for the matching sequence. Enzyme Codes: reports the numerical classification scheme for enzymes, based on the chemical reactions they catalyze. As a system of enzyme nomenclature, every EC number is associated with a recommended name for the respective enzyme. Missing data symbol: empty cell