This Electrophysiological recordings and a liposome-based assay showing the absence of chloride and proton conduction in TTYH proteins_Readme.txt file was generated on 2021-07-21 by Anastasiia Sukalskaia GENERAL INFORMATION 1. Title of Dataset: Electrophysiological recordings and a liposome-based assay showing the absence of chloride and proton conduction in TTYH proteins 2. Author Information A. Principal Investigator Contact Information Name: Raimund Dutzler Institution: University of Zurich Address: Winterthurerstrasse 190, Zurich 8057, Switzerland Email: dutzler@bioc.uzh.ch B. Alternate Contact Information Name: Anastasiia Sukalskaia Institution: University of Zurich Address: Winterthurerstrasse 190, Zurich 8057, Switzerland Email: a.sukalskaia@bioc.uzh.ch 3. Date of data collection (single date, range, approximate date) October 2020 - June 2021 4. Geographic location of data collection : Winterthurerstrasse 190, Zurich 8057, Switzerland SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: no 2. Links to publications that cite or use the data: https://www.nature.com/articles/s41467-021-25106-4 DATA & FILE OVERVIEW 1. File List: TTYH_absence_of_chloride_conduction.xlsx - data from electrophysiological recordings showing the absence of chloride conduction in TTYH proteins; TTYH_absence_of_proton_conduction.xlsx - data from electrophysiological recordings and a liposome-based assay showing the absence of proton consuction in TTYH proteins. Electrophysiological recordings showing the absence of chloride and proton conduction in TTYH proteins.zip - raw data from electrophysiological recordings showing the absence of chloride and proton conduction in TTYH proteins The zip file contains the following folders: - HEK293_chloride_currents; - TTYH1_Calcium_chloride_currents; - TTYH1_Calcium_proton_currents; - TTYH1_chloride_currents; - TTYH2_Calcium_chloride_currents; - TTYH2_Calcium_proton_currents; - TTYH2_chloride_currents; - TTYH3_Calcium_chloride_currents; - TTYH3_Calcium_proton_currents; - TTYH3_chloride_currents; - VRAC--_AC_chloride_currents; - VRAC--_AQP4_chloride_currents; - VRAC--_AQP4_TTYH1_chloride_currents; - VRAC--_Calcium_chloride_currents; - VRAC--_Calcium_proton_currents; - VRAC--_chloride_currents. All files with raw electrophysiological data are ABF files and can be opened with Clampfit software. 2. Folder VRAC--_AQP4_chloride_currents was updated on 14 August 2021 (files 2 and 5 were replaced with correct ones). METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: TTYH_absence_of_chloride_conduction.xlsx: whole-cell electrophysiological recordings TTYH_absence_of_proton_conduction.xlsx: whole-cell electrophysiological recordings and a liposome-based assay for detection of proton transport in membrane proteins Lee, S. Y., Letts, J. A. & MacKinnon, R. Functional reconstitution of purified human Hv1 H+ channels. J Mol Biol 387, 1055-1060, doi:10.1016/j.jmb.2009.02.034 (2009). 2. Methods for processing the data: All electrophysiological data were analyzed in Clampfit 10.7 (Molecular Devices).