Information given below contains aligned DNA sequences and scripts used in Díaz et al. (Divergence and biogeography of the recently evolved Macaronesian red Festuca (Gramineae) species inferred from coalescence-based analyses). Data is shown in sections, including: 1) DNA sequences 2) IM analysis (scripts for IM program and an excel file with a summary of results) 3) Lagrange Biogeographic Analysis (Python scripts for Lagrange and an excel file with a summary of results) 4) p_distance analysis (Scripts for performing coalescence-based simulations using Mesquite, Simcoal and Paup) 5) Slatkin and Maddison Test (Scripts for performing coalescence-based simulations using Mesquite, Simcoal and Paup) Each section contains its own ReadMe file. Some considerations must be observed: a) Cmd.exe scripts work if the Windows 7 root directory is f: You can change this directory, but most scripts have to be changed accordingly too. b) The following executables are needed to run our CMD scripts (Please read web links): Cmd.exe ------> Microsoft Corporation. 2009. version 6.1.7600. Copyright (c). http://www.microsoft.com/resources/documentation/windows/xp/all/proddocs/en-us/ntcmds_shelloverview.mspx?mfr=true Munge.exe ------> Microsoft Corporation. version 5.2.3630.0. Copyright (c). PLEASE READ: http://ss64.com/nt/munge.html http://technet.microsoft.com/en-us/library/cc767868.aspx http://www.corrupteddatarecovery.com/File-Data/munge.exe-Microsoft-Windows-Operating-System-Microsoft-Corporation-5.2.3630.0.asp http://www.microsoft.com/download/en/details.aspx?id=19109 Calc.exe ------> Microsoft Corporation. Copyright (c). PLEASE READ: http://download.corrupteddatarecovery.com/download-file/calc.exe http://www.corrupteddatarecovery.com/File-Data/calc.exe-Microsoft-Windows-Operating-System-Microsoft-Corporation-6.1.7600.16385.asp SimCoal.v.No.1 (Simcoal.exe) ------> http://cmpg.unibe.ch/software/simcoal/ http://www.cmpg.iee.unibe.ch/content/softwares__services/computer_programs/simcoal2/index_eng.html c) Software packages used in Analysis (Please read web links): Mesquite ------> http://mesquiteproject.org/mesquite/mesquite.html PAUP* ------> http://paup.csit.fsu.edu/ NOTE: p-distance and Slatkin and Maddison Test analysis need that some executables, like Munge.exe, Calc.exe and Simcoal.exe be placed within some folders. See "0 README BEFORE ANALYSIS.txt" files for detailed instructions.