Microsatellite data 28 loci from 9 populations.xls Microsatellite data from Boechera stricta for 28 loci from 9 populations in Idaho and Montana. Missing data are recorded as zero, otherwise the allele size is indicated in nucleotides. Contact person who collected these data: Bao-Hua Song Indiv: individual identifier Pop: Population name Subsequent columns indicate loci , which are paired, for diploid individuals Missing data are coded as zeroes ------------ CO endogenous leaf damage.xls Levels of herbivory damage on endogenous plants at the Round Mountain field site, Crested Butte, Colorado. Missing data are blank. Contact person who collected these data: Jill Anderson Percent leaf area removed: level of damage by herbivores ------------ BCMA allele-specific expression.xls Pyrosequencing allele-specific expression data for BCMA. Missing data are blank. Contact person who collected these data: K. Prasad plant: plant ID number BCMA: genotyped at the BCMA locus, with CC, CM, MM indicating Colorado homozygote, Colorado-Montana heterozygote, and Montana homozygote, respectively SNP: SNP scored in pyrosequencing Expression: quantitative expression level BCMA3_copies: number of copies of BCMA3 Adjustment Factor: controls for sensitivity of SNP_127A vs. SNP_294A (see text for details) Allelic_expression: quantitative expression level per allele in pyrosequencing ------------ BCMA heterologous expression.xls In vitro enzyme activity of BCMA genes expressed in E. coli. Missing data are blank. Contact person who collected these data: Carrie Olson-Manning Construct: Label for expression construct or control BCMA: CYP79 or BCMA gene or control or site directed mutagenesis construct BCMA_genes: CYP79 or BCMA gene or control Substrate: ILE or VAL Expression: quantitative level of enzyme activity in nmol product/ minute/ nmol cytochrome P450) ------------ BCMA fine mapping.xls Marker genotypes used in fine mapping of BCMA3. Contact person who collected these data: K. Prasad ID: Plant ID BStES0012 - BStES0012: Molecular marker names. Uppercase letters indicate scored genotypes, while lowercase indicates imputed genotypes. C, H, M indicate Colorado homozygote, heterozygote, and Montana homozygote, respectively. BC_ratio: concentration of branched chain-derived glucosinolates/concentration of total aliphatic glucosinolates ------------ BCMA2 genotypes & linkage.xls Marker genotypes used in fine mapping of BCMA2. Contact person who collected these data: K. Prasad RIL line: recombinant inbred line identifier Marker genotypes are show at BCMA2 and adjacent flanking markers. 'CC', 'CM', 'MM', and '-' indicate Colorado homozygote, heterozygote, Montana homozygote, and missing data, respectively. ------------ CO manipulative.xls Herbivory damage levels in the manipulative experiment at the Round Mountain field site, Crested Butte, Colorado. Missing data are blank. Contact person who collected these data: Jill Anderson Fam: family identifier Individual: Plant ID Block: spatial blocking variable Treat: treatment: 'c' = control, 'd' = damage Initial # leaves: initial number of leaves Survivorship: after herbivory, 0 = dead, 1 = alive Plant cover: local cover of living and dead vegetation within 5 cm of focal plant, scored as 0 = 0%, 1 = 10%, 2 = 20%, etc. Vegetation: percent local cover of living and dead vegetation within 5 cm of focal plant Fitness_viability: relative plant fitness based on survival, with mean = 1.0 ------------ GS transgenics.xls Glucosinolate phenotypes of BCMA genes expressed in Arabidopsis thaliana. Missing data are blank. Glucosinoate concentrations are in micro mol/gram fresh weight. Contact person who collected these data: K. Prasad Sample: sample ID ID: transgene or empty vector control Trt: treatment, including transgene or empty vector control Tot_Iso: concentration of total isoleucine-derived glucosinolates Tot_Met: concentration of total methionine-derived glucosinolates Tot_Val: concentration of total valine-derived glucosinolates Tot_GS: concentration of total aliphatic glucosinolates ------------ Lab Boechera_East GS.xls Glucosinolate phenotypes of Boechera stricta plants from 9 populations in Montana and Idaho. Missing data are blank. Contact person who collected these data: T. Mitchell-Olds Block: block in randomized complete block design Flat: flat in laboratory experiment Genotype: unique genotype identifier Population: source population Family: family within source population Wt: sample weight in mg. BC-ratio: concentration of branched chain-derived glucosinolates/concentration of total aliphatic glucosinolates TotGS: total glucosinolate concentration LogTotGS: log transformed total glucosinolates ------------ Lab Boechera_East Tni.xls Phenotypes for herbivory damage by Trichoplusia ni feeding of Boechera stricta plants from 9 populations in Montana and Idaho. Missing data are blank. Contact person who collected these data: T. Mitchell-Olds Flat: flat in laboratory experiment Family: genotype identifier Pop: source population Fam: family within source population Leaves: leaf number TniDamgPC: Trichoplusia ni leaf damage percentage ------------ Lab Data for Tni TotGS BCratio histogram.xls Phenotypes for glucosinolates and Trichoplusia ni herbivory of Boechera stricta plants from 9 populations in Montana and Idaho. Missing data are blank. Glucosinoate concentrations are in micro mol/gram fresh weight. Contact person who collected these data: T. Mitchell-Olds Herbivore damage: percent leaf area removed by Trichoplusia ni herbivory BC-ratio: concentration of branched chain-derived glucosinolates/concentration of total aliphatic glucosinolates TotGS: total glucosinolate concentration ------------ MT&CO Plant Means BC-ratio.xls Glucosinolate phenotypes for lab-grown plants from near Lost Trail Pass, Montana, and Round Mountain, Crested Butte, Colorado. Missing data are blank. Contact person who collected these data: K. Prasad Plant: Plant ID LSMean BCratio: least-squares mean glucosinolate branched-chain ratio Std Error: standard error of the mean BC-group: glucosinolate production derived from methionine or branched chain amino acids ------------ NILs 2008 BCMA GS Traits.xls Field phenotypes from F6 near-isogenic lines grown near Lost Trail Pass, Montana. Missing data are blank. Contact person who collected these data: K. Prasad and Bao-Hua Song Site: research site in Colorado or Montana Quad: spatial blocking variable Family: NIL family BCMA: genotype at BCMA locus, ÒCCÓ = Colorado homozygote, "CM" = heterozygote, ÒMMÓ = Montana homozygote BCratio: concentration of branched chain-derived glucosinolates/concentration of total aliphatic glucosinolates Leaves1: leaf number at 1st census DamagePropn1: proportion of leaf area removed by herbivores at the 1st census Alive1: alive or dead at the 1st census (1 or 0, respectively) Leaves2: leaf number at the 2nd census DamagePropn2: proportion of leaf area removed by herbivores at the second census Alive2: alive or dead at the second census (1 or 0, respectively) Herb_factor: 1st principal component of DamagePropn1 and DamagePropn2 Vegetation: percentage local cover both living and dead vegetation within 5 cm of focal plant MeanDamag: mean damage level from 1st and 2nd censuses MaxDamagPropn: maximum damage level during two censuses ------------ NILs MT&CO Herbivory.xls Field herbivory phenotypes from F6 near-isogenic lines grown near Lost Trail Pass, Montana. Missing data are blank. Contact person who collected these data: T. Mitchell-Olds Site: research site in Colorado or Montana Quad: spatial blocking variable Family: NIL family LeafNum1: leaf number at 1st census DamagePropn1: proportion of leaf area removed by herbivores at the 1st census LogHerb1: log transformed DamagePropn1 Alive2: alive or dead at the second census (1 or 0, respectively) Vegetation: percentage local cover both living and dead vegetation within 5 cm of focal plant ------------ RILs MT&CO FamMeans BCMA.xls Field phenotypes from F6 recombinant inbred lines grown near Lost Trail Pass, Montana and Round Mountain, Crested Butte, Colorado. Data are family means. Missing data are blank. Contact person who collected these data: T. Mitchell-Olds and Jill Anderson Site: experimental site in Montana or Colorado FamNum: family number Fam: recombinant inbred line family Adj_Jul_FD: Julian flowering date, see Anderson, et al. (2011). "Life history QTLs and natural selection on flowering time in Boechera stricta, a perennial relative of Arabidopsis." Evolution 65: 771-787. Propn_Flrd: Least Square Mean estimates of the proportion of plants that flowered within each family. Negative values occur when LSMeans are slightly below zero. MaxDamage%: maximum percent leaf area removed during the growing season MaxLN: maximum leaf number during the growing season Siliques: Least Square Mean estimates of the number of fruits produced. Negative values occur when LSMeans are slightly below zero. Fitness: relative fitness, calculated as the number of fruits/mean number of fruits BCMA_nohets: BCMA genotype, excluding heterozygotes. LL = Montana homozygote, SS = Colorado homozygote. ------------ RILs MT&CO indiv plants BCMA.xls Field phenotypes from F6 recombinant inbred lines grown near Lost Trail Pass, Montana and Round Mountain, Crested Butte, Colorado. Data are from individual plants. Missing data are blank. Contact person who collected these data: T. Mitchell-Olds and Jill Anderson Family: recombinant inbred line family Quad: spatial block in randomized complete block design Adj_Jul_FD: Julian flowering date, see Anderson, et al. (2011). "Life history QTLs and natural selection on flowering time in Boechera stricta, a perennial relative of Arabidopsis." Evolution 65: 771-787. MaxLN: maximum leaf number during the growing season MaxDamage%: maximum percent leaf area removed during the growing season LogMaxHerbivory: log transforms maximum herbivory Siliques: number of fruits produced Fitness: relative fitness, calculated as the number of fruits/mean number of fruits Vegetation: local cover both living and dead vegetation within 5 cm of focal plant, scored as 0 = 0%, 1 = 10%, 2 = 20%, etc. BCMA_nohet: BCMA genotype, excluding heterozygotes. LL = Montana homozygote, SS = Colorado homozygote. ------------ Parker x Ruby GS data.xls Glucosinolate phenotypes of lab-grown plants from cross between genotypes Parker (near Challis, Idaho) and Ruby (near Wisdom, Montana). Location details are in the Prasad et al paper. Data are F3 family means based on pooled F4 plants. Only homozygous families are included. Contact person who collected these data: C-R Lee and K. Prasad. Sample: Sample number Plate: HPLC plate identifier BCMA genotype: genotype at BCMA3 locus, either "Parker" or "Ruby" BCratio: concentration of branched chain-derived glucosinolates/concentration of total aliphatic glucosinolates