*************************** WING_BODY ALLOMETRY PACKAGE ***************************** THE WING-BODY ALLOMETRY PACKAGE ACCOMPANIES: Stillwell, R.C., Shingleton, A.W., Dworkin, I. Frankino, A.W., 2016. Tipping the scales: Evolution of the allometric slope independent of average trait size. Evolution. in press. The package contains a copy of the main data file, R Scripts use to analyze the data, and additional derived data files. Please contact the corresponding authors (A.W. Shingleton and W.A. Frankino) if you have any comments on the files. Please also let us know if you use the data in any additional publications. ************************************ CONTENTS *************************************** All files are plain ASCII text files, with the exception of Vector_Projections.xlxs. Text files with data have TABs as their field delimiters. 1. README.txt: This file. Includes a description of the variables for the data tables. 2. ALLDATA.txt: The complete data file giving the wing and body (pupal) size of each measured individual. 3. SLOPE_ANALYSIS.R: The annotated R scripts used in the main analyses. 4. VECTOR_PROJECTIONS.xlsx: The data file giving the inter-generational change in body (pupal) and wing size parallel and orthogonal to the allometry of the previous generation. Cells include formulae used to calculate the change. ************************************************************************************* Variable names and descriptions for ALLDATA.txt lineage - The experimental lineage subject to selection. Lineages A,B, and C were subject to hyperallometric selection; D, E, and F subject to hypoallometric selection; and G, H and I were the control lineages. X is initial population. sex - Sex of the measured fly. pup - Cross-sectional area of the pupal case in mm^2. wing - Area of wing in mm^2 gen - Generation starved - Feeding treatment. d0 = late-starved larvae, that were removed from their food at or just prior to the wandering stage; d1 = mid-starved larvae, which were removed from their food toward the middle of their final growth instar; and d2 = early-starved larvae, that were removed from their food c. 48h before wandering lingen - Lineage and generation ************************************************************************************* Description of SLOPE_ANALYSIS.txt This file contains the annotated R scripts used to conduct the analysis. The analysis can be applied directly to the data table in ALLDATA.txt ************************************************************************************* Variable names and descriptions for VECTOR_PROJECTIONS.txt This file contains the data used to test for evolutionarily independent change in the bivariate mean, as described in Figure S2. lineage - The experimental lineage subject to selection. Lineages A,B, and C were subject to hyperallometric selection; D, E, and F subject to hypoallometric selection; and G, H and I were the control lineages. sex - Sex of the measured fly. gen - Generation n - Number of flies of specified lineage, sex and generation logpupal - Mean log pupal case size (mm^2) within each lineage/sex/generation combination. logwing - Mean log wing area (mm^2) within each lineage/sex/generation combination. dpupal - Change in mean log pupal case size from previous generation for each lineage/sex combination. dwing - Change in mean log wing are from previous generation for each lineage/sex combination. l - Used for calculation of uniform vector. slope - Slope (RMA) of the wing-body allometry in the previous generation for each lineage/sex combination. upupal - The pupal component of a unit vector in the direction of the wing-body allometry in the previous generation for each lineage/sex combination. uwing - The wing component of a unit vector in the direction of the wing-body allometry in the previous generation, for each lineage/sex combination. dotprod - The dot product of the unit vector (upupal, uwing) in the direction of the wing-body allometry in the previous generation, and the vector describing the observed change in pupal and wing size from the previous generation (dpupal, dwing), for each lineage/sex combination. parpup - The pupal component of (dpupal,dwing) that is in the direction of (upupal, uwing). parwing - The wing component of (dpupal,dwing) that is in the direction of (upupal, uwing). orthpup - The pupal component of (dpupal,dwing) that is orthogonal to direction of (upupal, uwing). orthwing - The wing component of (dpupal,dwing) that is orthogonal to direction of (upupal, uwing). normparpup - The pupal component of (dpupal,dwing) that is in the direction of (upupal, uwing), normalized for change in the control lineages. normparwing - The wing component of (dpupal,dwing) that is in the direction of (upupal, uwing), normalized for change in the control lineages. normorthpup - The pupal component of (dpupal,dwing) that is orthogonal to direction of (upupal, uwing), normalized for change in the control lineages. normorthwing - The wing component of (dpupal,dwing) that is orthogonal to direction of (upupal, uwing), normalized for change in the control lineages.