Data from: Comparative analysis to investigate a possible mechanism for cell enlargement in succulent leaves of Crassothonna capensis (Asteraceae)
Abstract
This dataset was used for Fig. 2 in "Comparative analysis to investigate a possible mechanism for cell enlargement in succulent leaves of Crassothonna capensis (Asteraceae)". Succulent plants, characterized by the presence of water-storage tissues, often exhibit distinctive leaf morphology. However, their developmental mechanisms remain largely unknown, partly due to the lack of an appropriate model plant. In this study, we evaluated the potential of Asteraceae species as a model system for investigating the mechanisms underlying leaf succulence. First, we analyzed the leaf anatomical and cellular characteristics of succulent plants in the genera Caputia, Crassothonna, Curio, Othonna, and Senecio. This DRYAD data shows that the internal cell size is much larger in succulent species than in normal-leaved species.
Dataset DOI: 10.25338/B8ZW7S
Corresponding Author Contact Information:
Name: Hokuto Nakayama
Institution: The University of Tokyo
Email: hokuto@bs.s.u-tokyo.ac.jp
Description of the data and file structure
This dataset contains cell size measurements from mature leaves of Senecioneae and Othonninae species. This data was used for Fig. 2: Comparison of leaf cell size in Senecioneae and Othonninae species. Images of Technovit sections were used to measure epidermal and inner cell sizes in leaves. Cells in the outermost layer of each organ and fully expanded inner cells were selected as the epidermal and inner cells, respectively. To calculate cell area, six epidermal and inner cells in an image of the section were used. The experiments were performed three times with independent individuals (n = 18 from 3 individuals).
All data is included in cell.csv.
Files and variables
File: cell.csv
Description: Raw data of cell size measurement in mature leaves.
Variables
- sp: plant species name - epi = epidermal cell, inn = inner cells
- svul = Senecio vulgaris
- sant = Senecio antandroi
- crep = Curio repens
- craw = Curio rowleyanus
- oeup = Othonna euphorbioides
- ccap = Crassothonna capensis
- area: µm2
Images of the sections were used to measure epidermal and palisade cell sizes in leaves. Cells in the outermost layer of the leaf image and fully expanded inner cells were selected as the epidermal and leaf inner cells, respectively. Cell areas were calculated using Fiji (ImageJ2; ver. 2.9.0). To calculate cell area, six epidermal and inner leaf cells in each image were used as biological replicates. Then, the average leaf area (n = 3) was measured.
