Data from: Nematode mind: Exploring the role of the RNAi pathway in learning, memory and beyond
Data files
Jun 12, 2025 version files 51.01 KB
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AppetitiveLearning.csv
958 B
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AppetitiveLearning.R
1.82 KB
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AversiveLearning.csv
826 B
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AversiveLearning.R
2.35 KB
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Development.R
2.58 KB
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DevelopmentHT115.csv
253 B
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DevelopmentOP50.csv
369 B
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Eggs.csv
9.59 KB
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EggSize.R
1.52 KB
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HT115reproduction.csv
5.95 KB
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HT115reproduction.R
4.57 KB
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HT115survival.csv
1.06 KB
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lifespanHT115.R
1.33 KB
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lifespanOP50.R
1.40 KB
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OP50reproduction.csv
6.10 KB
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OP50reproduction.R
4.54 KB
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OP50survival.csv
1.07 KB
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README.md
4.72 KB
Abstract
Caenorhabditis nematodes, particularly the well-known C. elegans, have challenged early views of them as hard-wired by demonstrating diverse learning and memory capabilities. This cognitive repertoire, developed with just several hundred neurons, highlights the evolutionary importance of cognitive traits. In this study, we examine the relationship between learning, development, reproduction, and lifespan, focusing on its regulation by the conserved RNA interference (RNAi) pathway. Specifically, we examine NRDE-3, a key Argonaute protein in this pathway, using an RNAi-defective nrde-3 mutant to evaluate the effects of this mutation under two diets. Here we recite the relevant phenotypic data from the assays, as well as the R scripts used for their analyses.
Files and variables
File: OP50reproduction.csv
Description: Daily reproduction scoring of individuals fed with E. coli OP50
Variables
- Strain: Genotype wild-type or mutant(N2/NRDE-3)
- Plate: Increasing number of plates for the two genotypes respectively
- Day: Days of counting offspring (0-7)
- Larvae: Daily hatched offspring numbers
File: HT115reproduction.csv
Description: Daily reproduction scoring of individuals fed with E. coli HT115 (L4440)
Variables
- Strain: Genotype wild-type or mutant(N2/NRDE-3)
- Plate: Increasing number of plates for the two genotypes respectively
- Day: Days of counting offspring (0-7)
- Larvae: Daily hatched offspring numbers
File: OP50survival.csv
Description: Lifespan assay for individuals fed with E. coli OP50
Variables
- Line: Genotype wild-type or mutant(N2/NRDE-3)
- Plate: Increasing number of plates for the two genotypes respectively
- Day: Days of scoring death events
- Event: =1 when individual is dead
- Matricide: =1 when individual died due to matricide
- comment: Additional comments. =L when individual is lost, =K when individual was killed during transfer
File: HT115survival.csv
Description: Lifespan assay for individuals fed with E. coli HT115 (L4440)
Variables
- Line: Genotype wild-type or mutant(N2/NRDE-3)
- Plate: Increasing number of plates for the two genotypes respectively
- Day: Days of scoring death events
- Event: =1 when individual is dead
- Matricide: =1 when individual died due to matricide
- comment: Additional comments. =L when individual is lost, =K when individual was killed during transfer
File: DevelopmentHT115.csv
Description: Proportion of fully developed individuals fed with E. coli HT115 (L4440)
Variables
- Strain: Genotype wild-type or mutant(N2/NRDE-3)
- No: Replication plate number for the respective genotype
- Mature: Number of individuals with fully formed vulvas
- Nonm: Number of individuals with non fully formed vulvas
- Total: Total number of individuals on plate
- Time: Timepoint of observation (50/51/52)
File: DevelopmentOP50.csv
Description: Proportion of fully developed individuals fed with E. coli OP50
Variables
- Strain: Genotype wild-type or mutant(N2/NRDE-3)
- No: Replication plate number for the respective genotype
- Mature: Number of individuals with fully formed vulvas
- Nonm: Number of individuals with non fully formed vulvas
- Total: Total number of individuals on plate
- Time: Timepoint of observation (47/48/49)
File: AversiveLearning.csv
Description: Chemotaxis assay for worms conditioned to avoid butanone
Variables
- Block: Experimental block
- Line: Genotype wild-type or mutant(N2/NRDE-3)
- Treatment: Treatment during conditioning phase C= naive individuals, L= trained individuals
- Replicate: Replication within line and tratment combinations
- But: Individuals counted on the butanone area
- Alc: Individuals counted on the ethanol area
- Init: Individuals on the starting point that the individuals were placed at the beginning of the assay
- Total: Total number of individuals on plate
File: AppetitiveLearning.csv
Description: Chemotaxis assay for worms conditioned to be attracted to butanone
Variables
- Block: Experimental block
- Line: Genotype wild-type or mutant(N2/NRDE-3)
- Treatment: Treatment during conditioning phase C= naive individuals, L= trained individuals
- Replicate: Replication within line and tratment combinations
- But: Individuals counted on the butanone area
- Alc: Individuals counted on the ethanol area
- Init: Individuals on the starting point that the individuals were placed at the beginning of the assay
- Total: Total number of individuals on plate
File: AversiveLearning.R
Description: R code for data analysis of aversive learning assays
File: AppetitiveLearning.R
Description: R code for data analysis of appetitive learning assays
File: Development.R
Description: R code for data analysis of development assays for both food sources
File: HT115reproduction.R
Description: R code for data analysis of reproduction assays for individuals fed with E. coli HT115.
File: OP50reproduction.R
Description: R code for data analysis of reproduction assays for individuals fed with E. coli OP50.
File: lifespanHT115.R
Description: R code for data analysis of lifespan assays for individuals fed with E. coli HT115.
File: lifespanOP50.R
Description: R code for data analysis of lifespan assays for individuals fed with E. coli OP50.
File: EggSize.R
Description: R code for data analysis of egg size assays.