Intestinal macrophages modulate synucleinopathy along the gut-brain axis
Data files
Dec 03, 2025 version files 20.12 KB
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GI_HKNorm_Data_Final.xlsx
18.22 KB
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README.md
1.90 KB
Abstract
We generated a digital spatial profiling (DSP) dataset from duodenal myenteric plexus of 3-month-old 3KL and WT mice, analyzing 2–3 ROIs per slide across 2 animals per genotype. The dataset comprises expression values for 25 proteins, including PD-associated targets, lysosomal and autophagy markers, internal housekeeping and background controls. Normalized digital counts were obtained per ROI, enabling comparative analysis of protein signatures between genotypes at an early stage of pathology
Dataset DOI: 10.5061/dryad.12jm63zb6
Description of the data and file structure
The dataset was generated using digital spatial profiling of duodenal myenteric plexus from 3-month-old 3KL and WT mice to capture early molecular changes in enteric synucleinopathy. These data were collected to assess how early α-synuclein pathology correlates with lysosomal stress in the ENS.
5 μm thick FFPE sections of the myenteric plexus were deparaffinized and incubated with antibodies against bIII-tubulin to select myenteric ganglia as segmented ROI, according to the manufacturer’s instructions. Cell nuclei were stained with DAPI. Each ROI was manually drawn around myenteric ganglia in the myenteric plexus (geometric segments), encompassing both neurons and glial cells, resulting in a total of 2-3 analysed ROIs per slide. Each slide was incubated with an antibody panel containing antibodies conjugated to a unique UV-photocleavable oligonucleotide tag. Selected ROIs were individually illuminated with UV light using NanoString's GeoMx™ Digital Spatial Profiler System (NanoString Technologies).
Files and variables
File: GI_HKNorm_Data_Final.xlsx
Description:
Variables
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Genotype: 3KL vs. WT mice
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ME 3KL= 3KL_55A_24_GI_3mo_scan1_001 3KL_55A_24_GI_3mo_scan1_002 3KL_55A_24_GI_3mo_scan1_003
3KL56_GI_PD_001 3KL56_GI_PD_002 3KL56_GI_PD_003
ME WT= WT_292A_32_GI_3mo_scan1_001 WT_292A_32_GI_3mo_scan1_002
WT_293A_38_GI_3mo_001 WT_293A_38_GI_3mo_002 WT_293A_38_GI_3mo_003
LP 3KL= 3KL_55A_24_GI_3mo_scan1_004 3KL_55A_24_GI_3mo_scan1_005
3KL56_GI_PD_004 3KL56_GI_PD_005
LP WT= WT_292A_32_GI_3mo_scan1_003 WT_292A_32_GI_3mo_scan1_004
WT_293A_38_GI_3mo_004 WT_293A_38_GI_3mo_005
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Protein expression values: normalized digital counts per ROI