Squash preparations and living cells showing chromosome arrangement in mitosis, meiosis I, and meiosis II in green lacewing (Chrysoperla rufilabris) males
Data files
Jul 26, 2025 version files 2.57 GB
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C-rufilabris-male-cell-division_Dryad-upload_07-23-2025.zip
2.57 GB
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README.md
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Abstract
In previously-studied species of insects in the order Neuroptera, sex chromosomes in males exhibit a phenomenon called "distance segregation," where the X and Y chromosomes are separated and associated with opposite poles from prometaphase I-anaphase I. This study aimed to determine the chromosome number and sex determination of a previously unstudied Neuropteran insect, the green lacewing Chrysoperla rufilabris (Burmeister, 1839). Following determination of chromosome number and sex determination we examined images of fixed and living specimens to determine if distance segregation was observed. This dataset includes all of the raw images of living and aceto-orcein squashed cells that were observed to determine chromosome number, sex determination, and existence of distance segregation.
Dataset DOI: 10.5061/dryad.15dv41p8f
Description of the data and file structure
This dataset contains the raw image files required to study chromosome number, alignment, and position during mitosis, meiosis I, and meiosis II in adult males of the green lacewing Chrysoperla rufilabris (Burmeister, 1839). Images of aceto-orcein squashes of testes and living spermatocytes are included in this dataset. Preparations were imaged using a Nikon Eclipse TS100 microscope equipped with a 100X, 1.25 NA phase-contrast, oil immersion objective. Images of aceto-orcein squashes were collected using a View 4K HD camera (Microscope Central, Feasterville, PA, USA) using InFocus software version x64 (Microscope Central). Images of living cells were either collected using a View 4K HD camera using InFocus software version x64 (subfolder 03-19-2025), or a Spot camera with the Spot 3.5.7 software (SPOT Imaging, Sterling Heights, MI, USA). In images in the subfolder “Live-cell preps” in the sub-subfolders 02-19-2009, 04-14-2009, and 07-21-2009, 130 pixels=10 μm. In images in the subfolder “Live-cell preps” in the sub-subfolder 03-19-2025, 620 pixels=10 μm. In all images in the subfolder “Chromosome-squash preps” 620 pixels=10 μm.
Files and variables
File: C-rufilabris-male-cell-division_Dryad-upload.zip
Description: The zipped folder (C-rufilabris-male-cell-division_Dryad-upload_07-21-2025.zip) contains 3490 image files, all in jpeg format. The images are all of testes preparations from male Chrysoperla rufilabris.
Subfolder “Chromosome-squash preps” contains 778 files organized into 14 sub-subfolders that are named according to the date images sub-subfolder were obtained, in the format MM-DD-YYYY where M=month, D=day, and Y=year. Images of squashed cells in mitosis, meiosis I, and meiosis II are contained in these sub-subfolders. Within the sub-subfolders, the file naming convention is based on date/time imaged. File names are in the format YYYYMMDDHHMMSSsss, where Y=year, M=month, D=day, H=hour, S=second, and s=millisecond.
Subfolder “Live-cell preps” contains 2496 files organized into 4 sub-subfolders named in the format MM-DD-YYYY where M=month, D=day, and Y=year. All images in this subfolder are of living prometaphase I and metaphase I spermatocytes. File naming conventions are as follows:
In the “02-19-2009” sub-subfolder, images in the “cell 01” sub-sub-subfolder are named with the prefix cell01 followed by the number in the series starting with 000 and ending with 785.
In the “03-19-2025” sub-subfolder, all image file names are in the format YYYYMMDDHHMMSSsss, where Y=year, M=month, D=day, H=hour, S=second, and s=millisecond.
In the “04-14-2009” sub-subfolder, all images are named with the prefix “cell01” followed by the number in the series starting with 000 and ending with 292.
In the “07-21-2009” sub-subfolder, within the “Cell 01” sub-sub-subfolder all images are named with the prefix “Cell one” followed by the number in the series, starting with 000 and ending with 1030.
Code/software
Images can be viewed using any image viewer. Images can be viewed and analyzed using ImageJ (https://imagej.net/ij/)
Access information
Other publicly accessible locations of the data:
- NA
Data was derived from the following sources:
- NA
Testes of Chrysoperla rufilabris were removed from adult male abdomens.
For aceto-orcein squashes, isolated testes were fixed in 3:1 ethanol/acetic acid for 5 minutes, rinsed 30 seconds in distilled water, and stained in filtered aceto-orcein (2.5 g orcein in 100 mL 45% (v/v) acetic acid) for 3 minutes. Stained testes were placed on a slide in a drop of 45% acetic acid. A coverslip was placed over the testes and uniform pressure was applied by hand on the coverslip.
For living-cell preparations, testes were placed under a thin layer of Kel-F #10 oil (Ohio Valley Specialty Company, Marietta, Ohio) in a culture chamber on a coverslip. Testes contents were spread under the oil and living cells were immediately imaged.
Imaging was done on a Nikon Eclipse TS100 microscope (Nikon Instruments, Tokyo, Japan) equipped with a 100X, 1.25 NA phase-contrast, oil immersion objective. Images were collected either with a View 4K HD camera (Microscope Central, Feasterville, PA, USA), using InFocus software version x64 (Microscope Central), or using a Spot camera with the Spot 3.5.7 software (SPOT Imaging, Sterling Heights, MI, USA).