https://doi.org/10.5061/dryad.1g1jwsv37

Uploaded data set contains the lipidomics files from LC-MS/MS Orbitrap Instrument.

Description of the data and file structure

General Lipidomics files are named as follows:

For cells – CellName-GeneName-Genotype-ModeofIonization. For example “HMC3-WT-NegativeMode” - refers to lipidomics data recorded for HMC3 wild type cells in negative mode of ionization. Genotype = Wild Type (WT), knock out (KO), Add-Back (PLD3 KO+ stable expression of PLD3 for rescue)

For tissues – TissueName-GeneName-Genotype-ModeofIonization. For example “Spleen-PLD4-WT-NegativeMode” - refers to lipidomics data recorded for Spleen from PLD4 wild type mice in negative mode of ionization. Genotype = Wild Type (WT), knock out (KO), hetereozygous (HET).

BMP measurement files are named with initials “BMP”. For example - “BMP-HMC3-WT-NegativeMode” - refers to the BMP measurements from HMC3 cells with wildtype genotype in negative mode of analysis. WT = wildtype, KO = Knock out

PLD3 and PLD4 are two genes.

Negative mode and positive mode - refers to the mode of ionization in which data was recorded on the Orbitrap.

Sharing/Access information

Not Applicable. 

Code/Software

Excalibur or LipidSearch is required to open these files. For general lipidomics, output of LipidSearch can be analysed in lipidcruncher available here: https://lipidcruncher.streamlit.app