Neural crest-mediated Gas1 expression regulates species-specific jaw size during development and evolution
Data files
Jul 28, 2026 version files 52.50 GB
-
D24_1.fastq.gz
3.12 GB
-
D24_2.fastq.gz
3.01 GB
-
D24_3.fastq.gz
2.62 GB
-
D24Gas1MO_10.fastq.gz
2.68 GB
-
D24Gas1MO_13.fastq.gz
3.24 GB
-
D24Gas1MO_14.fastq.gz
3.14 GB
-
D24Gas1OE_10.fastq.gz
3.55 GB
-
D24Gas1OE_11.fastq.gz
3.61 GB
-
D24Gas1OE_9.fastq.gz
2.33 GB
-
Q24_3.fastq.gz
2.21 GB
-
Q24_4.fastq.gz
3.40 GB
-
Q24_5.fastq.gz
2.55 GB
-
Q24Gas1MO_04.fastq.gz
1.98 GB
-
Q24Gas1MO_12.fastq.gz
2.62 GB
-
Q24Gas1MO_16.fastq.gz
3.27 GB
-
Q24Gas1OE_1.fastq.gz
3.32 GB
-
Q24Gas1OE_14.fastq.gz
3.15 GB
-
Q24Gas1OE_22.fastq.gz
2.69 GB
-
README.md
2.24 KB
Abstract
Developmental control of jaw size is crucial to prevent birth defects and facilitate evolutionary adaptation. We have shown that jaw size is established by neural crest mesenchyme (NCM), which are progenitor cells that migrate into the mandibular primordia and produce the jaws. NCM relies on multiple signaling pathways, including Sonic Hedgehog (SHH), to mediate interactions with mandibular epithelium and promote jaw outgrowth. Growth Arrest-Specific 1 (GAS1), which is a SHH co-receptor, is expressed 20-75 times higher in mandibular primordia of duck relative to those of quail. Gas1 overexpression and knockdown in NCM alters cell number and mandibular primordia size.
To analyze the species-specific differential expression of Gas1 regulates the Shh pathway as well as other signaling pathways, which direct mandibular primordia development, we overexpressed and knocked down GAS1 in white Pekin duck (Anas platyrhynchos domestica) and Japanese quail (Coturnix coturnix japonica) embryos. Mandibular primordia were collected at HH24 for mRNA seq. mRNA was sequenced using Illumina NovaSeq paired-end 150 bp sequencing.
Dataset DOI: 10.5061/dryad.1g1jwsvc5
Description of the data and file structure
Data are mRNA sequencing results from Illumina NovaSeq paired-end 150 bp sequencing. Mandibular primordia were collected at HH24 from Pekin duck (D, Anas platyrhynchos domestica) and Japanese quail (Q, Coturnix coturnix japonica) embryos for mRNA seq. Embryos were either control, overexpressing Gas1 (OE), or injected with Gas1 vivo-morpholino (MO).
Files and variables
File: D24Gas1OE_11.fastq.gz
Description: Duck HH24 Gas1 overexpression sample 11
File: Q24Gas1OE_1.fastq.gz
Description: Quail HH24 Gas1 overexpression sample 1
File: D24Gas1OE_10.fastq.gz
Description: Duck HH24 Gas1 overexpression sample 10
File: Q24_4.fastq.gz
Description: Quail HH24 sample 4
File: Q24Gas1MO_16.fastq.gz
Description: Quail HH24 Gas1 morpholino knockdown sample 16
File: Q24Gas1OE_14.fastq.gz
Description: Quail HH24 Gas1 overexpression sample 14
File: Q24Gas1OE_22.fastq.gz
Description: Quail HH24 Gas1 overexpression sample 22
File: D24_1.fastq.gz
Description: Duck HH24 sample 1
File: D24_2.fastq.gz
Description: Duck HH24 sample 2
File: Q24Gas1MO_12.fastq.gz
Description: Quail HH24 Gas1 morpholino knockdown sample 12
File: Q24_5.fastq.gz
Description: Quail HH24 sample 5
File: Q24_3.fastq.gz
Description: Quail HH24 sample 3
File: D24_3.fastq.gz
Description: Duck HH24 sample 3
File: D24Gas1OE_9.fastq.gz
Description: Duck HH24 Gas1 overexpression sample 9
File: Q24Gas1MO_04.fastq.gz
Description: Quail HH24 Gas1 morpholino knockdown sample 4
File: D24Gas1MO_14.fastq.gz
Description: Duck HH24 Gas1 morpholino knockdown sample 14
File: D24Gas1MO_10.fastq.gz
Description: Duck HH24 Gas1 morpholino knockdown sample 10
File: D24Gas1MO_13.fastq.gz
Description: Duck HH24 Gas1 morpholino knockdown sample 13
Code/software
Any software that can process RNA-seq reads should work.