Overview

This dataset contains behavioral and neural recordings supporting the manuscript:
“Complementary roles of dorsal and ventral hippocampus in the flexible adaptation of goal-directed behavior.”

Data include z-scored ΔF/F (delta F over F) calcium signals and behavioral performance measures from mice performing Y-maze, Barnes maze, and digging tasks.
Each Excel file corresponds to one figure in the paper, and each worksheet (tab) corresponds to one figure panel.
All figures are fully reproducible using MATLAB, GraphPad Prism, or Microsoft Excel.

File Structure

MATLAB Analysis Scripts

Main Figure Data Files

Figure1.xlsx

Contains z-scored ΔF/F fluorescence from dCA1 and vCA1 during the Y-maze task, focused on the transition zone and strategy-specific activity on Reversal Day 1.
Each sheet corresponds to a figure panel.

• Each column = mouse
• Each row = day or strategy condition
• Headings = recorded region (dCA1, vCA1)

Panel descriptions:

• panel d: ΔF/F across all days.
• panels e–h: Pairwise comparisons for acquisition day 3 (A3), reversal day 1 (R1), reversal day 3 (R3), and reversal day 6 (R6), respectively.
• panels i–l: ΔF/F during strategy use on reversal learning day 1 for vCA1 and dCA1, respectively.

Variables:

Notes: All signals are normalized (z-scored) per session and mouse.

Figure2.xlsx

Behavioral performance in the Y-maze task, showing the probability of strategy use when vCA1 or dCA1 was inhibited during acquisition or reversal learning.
Each worksheet (tab) corresponds to one panel in figure 2.

Panel descriptions:

• panel g: P(stay | lose) for vCA1 acquisition inhibition.
• panel h: P(stay | lose) for vCA1 reversal inhibition.
• panel i: P(shift | win) for vCA1 acquisition inhibition.
• panel j: P(shift | win) for vCA1 reversal inhibition.
• panel k: P(stay | lose) for dCA1 acquisition inhibition.
• panel l: P(stay | lose) for dCA1 reversal inhibition.
• panel m: P(shift | win) for dCA1 acquisition inhibition.
• panel n: P(shift | win) for dCA1 reversal inhibition.

Variables:

Figure3.xlsx

Data from the Barnes maze showing behavioral performance of the recorded mice and corresponding neural activity.

• Sheet 1: Total errors and perseverative errors.
• Sheet 2: Z-scored ΔF/F from vCA1 and dCA1 during the searching epoch.

Variables:

Figure4.xlsx

Behavioral performance in the Barnes maze when vCA1 or dCA1 was inhibited during acquisition or reversal learning.
Each sheet = one panel.

• Columns = mice (Control or DREADD)
• Rows = days

Panel descriptions:

• panels c–d: Total errors and perseverative errors for vCA1 acquisition inhibition.
• panels e–f: Total and perseverative errors for vCA1 reversal inhibition.
• panels g–h: Total and perseverative errors for dCA1 acquisition inhibition.
• panels i–j: Total and perseverative errors for dCA1 reversal inhibition.
• panels l–m: Strategy use percentages (global, serial, local, directed) for vCA1 acquisition inhibition.
• panels n–o: Strategy use for vCA1 reversal inhibition.
• panels p–q: Strategy use for dCA1 acquisition inhibition.
• panels r–s: Strategy use for dCA1 reversal inhibition.

Variables:

Figure5.xlsx

Z-scored ΔF/F fluorescence of vCA1 and dCA1 during the digging task.
Each sheet corresponds to one figure panel.

• Columns = mice
• Rows = days: Acquisition, Reversal, and Extra Dimensional Set Shifting.

Panel descriptions:

• panel c: Average z-scored ΔF/F across days (Acquisition, Reversal, and EDS).
• panels d–f: Pairwise comparisons for Acquisition, Reversal, and EDS, respectively.
• panel i: Z-scored ΔF/F during post-dig epoch after correct and incorrect trials for vCA1 and dCA1, respectively.

Variables:

Figure6.xlsx

Behavioral performance (trials-to-criterion; 8 correct trials out of 10 consecutive trials) during the digging task for control and inhibited mice.

• Columns = mice
• Rows = days or learning phases
• Sheets = Inhibition during different learning phases (Acquisition or Reversal).

Panel descriptions:

• panels c–d: Trials-to-criterion for vCA1 acquisition inhibition and vCA1 reversal inhibition, respectively.
• panels e–f: Trials-to-criterion for dCA1 acquisition inhibition and dCA1 reversal inhibition, respectively.

Variables:

Supplementary Figure Data Files

Supplementary_Figure2.xlsx

Z-scored ΔF/F from vCA1 and dCA1 in incorrect trials of the Y-maze transition zone.
Variables: dCA1, vCA1, Mouse, Day, ΔF/F (z-score)

Supplementary_Figure3.xlsx

Neural activity during strategy use in the Y-maze.
Each sheet includes vCA1 and dCA1 activity across behavioral strategies.
Variables: dCA1, vCA1, Strategy, Mouse, ΔF/F (z-score)

Supplementary_Figure4.xlsx

Contains outcome-related neuronal activity analyses from ventral CA1 (vCA1) and dorsal CA1 (dCA1) in the Y-maze task on Reversal Day 1.
The first set of tabs compares post-dig ΔF/F activity following correct versus incorrect trials for both vCA1 and dCA1.
The subsequent tabs include correlation analyses linking post-decision (post-dig) activity after incorrect trials with the neural activity during the next correct trial in the transition zone.
Variables::

Supplementary_Figure6.xlsx

Behavioral learning rates in Y-maze comparing control and inhibited mice during Acquisition and Reversal.
Variables: Control, DREADD, Day, Phase, Performance: Learning Rate

Supplementary_Figure7.xlsx

Adaptive strategy use in Y-maze (related to Figure 2).
Variables: Control, DREADD, Day, Strategy Use (%), Mouse

Supplementary_Figure8.xlsx

Latency and path length to locate the escape hole in the Barnes maze (related to Figure 4).
Variables: Control, DREADD, Day, Latency (s), Path Length (cm), Phase

Supplementary_Figure9.xlsx

Z-scored ΔF/F from vCA1 and dCA1 during investigation periods of wrong-choice trials in the digging task (related to Figure 5).
Variables: Region (dCA1, vCA1), Mouse, Days: ACQ, RL and EDS

Supplementary_Figure11.xlsx

Perseverative error signals in vCA1 and dCA1 on Acquisition and Extra-Dimensional Set-Shifting days (related to Figure 5).
Variables: Region (dCA1, vCA1), post-dig ΔF/F activity following correct trials compare to post-dig ΔF/F activity following the incorrect trials during Learning phase and EDS phase.

Supplementary_Figure12.xlsx

Results of ROC analysis from logistic regression predicting choice behavior from post-dig neural activity (related to Figure 5).
Variables: Region (vCA1/dCA1). AUC (calcium activity of each region in the current trial) of reversal learning day. Outcome of the next trial: 1 means the outcome was correct and 0 means the outcome was incorrect.

Notes on Excel Formatting

• Bold headers (e.g., Control, DREADD, dCA1, vCA1) identify variable names and experimental groups.
• Thin red divider lines separate data from different regions (dCA1/vCA1) or manipulations (control/inhibition) within the same sheet for visual clarity only.
• No color, shading, or font formatting encodes data values or metadata; these elements are purely cosmetic and do not affect data interpretation or re-analysis.
  All numeric data can be read directly by standard software (MATLAB, Prism or Excel) without loss of information.

Reproducibility

All datasets and results can be easily reproduced and visualized in MATLAB, GraphPad Prism, or Microsoft Excel.

Contact

Maryam Hasantash — mh4026@cumc.columbia.edu
Christoph Anacker — ca2694@cumc.columbia.edu