Data from: Integrative morphological, biogeographic and molecular evidence supports Azorella crenata and A. reniformis (Azorelloideae, Apiaceae) as distinct species
Data files
Jul 28, 2026 version files 251.17 KB
Abstract
This dataset comprises the aligned DNA sequence matrix of five chloroplast regions (rps16, trnQ–rps16, rps16–trnK, trnG–trnS, and rpL32–trnL) and the nuclear ribosomal ITS (nrITS), including coded indels, for species of Azorella section Laretia (= Diversifolia group sensu Fernández et al. 2017) and Azorella selago (Azorelloideae, Apiaceae). A total of twenty-five accessions were examined: eighteen were previously included in the molecular phylogenetic study of subfamily Azorelloideae by Fernández et al. (2017), and seven additional accessions were newly generated for the present study (Fernández, Beck & Calviño, 2026, Systematic Botany). This sampling includes 13 of the 14 species of Azorella sect. Laretia, and Azorella selago which was used to root the trees. For each species of A. sect. Laretia, at least two accessions were sampled when possible, to test the monophyly of the species.
Dataset DOI: 10.5061/dryad.2bvq83c55
Files and variables
File: Seq_alignment_cpDNAITSindels_Azorella_section_Laretia_MPE_2017__SB_2026.nex
Description: The file is in NEXUS format and was used for Bayesian Inference (BI) and Maximum Likelihood (ML) phylogenetic analyses. The matrix includes the total number of taxa, the total number of characters, specimen names with corresponding DNA numbers, and the aligned sequences for each specimen.
Newly generated DNA sequences were edited, assembled, and manually aligned with the data matrices used in Fernández et al. (2017). The cpDNA and ITS data matrices were analyzed both separately and in combination. A matrix of binary-coded indels was constructed for each data partition (i.e., the five cpDNA regions and ITS) to incorporate length-mutational information into the phylogenetic analyses. Gap coding followed Calviño and Downie (2007). The plastid DNA, nuclear DNA, and total evidence data matrices (including indels) were each analyzed using Maximum Likelihood (ML) and Bayesian Inference (BI) under the settings described in Calviño et al. (2008).
Code/software
The aligned sequence matrix is provided in NEXUS format and can be viewed and edited using standard phylogenetic software compatible with this format.
Access information
Previously published sequences incorporated into this dataset are publicly available in GenBank and were originally reported in Fernández et al. (2017). All newly generated DNA sequences have been deposited in GenBank, and their accession numbers are provided in Appendix 2 of the manuscript currently under review.