Data from: Dopamine and serotonin co-transmission filters striatonigral synaptic activity via 5-HT1B receptor activation
Data files
Oct 08, 2025 version files 27.30 MB
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Dataset_Molinari_etal.zip
27.29 MB
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README.md
15.07 KB
Abstract
The substantia nigra pars reticulata (SNr), a key basal ganglia output nucleus, is modulated by dopamine (DA), believed to be released locally from midbrain dopamine neurons. Although DA has been proposed to regulate GABA release from medium spiny neurons (MSN) terminals via presynaptic D1 receptors (D1Rs), the precise mechanisms remain unclear. Using presynaptic optical recordings of synaptic vesicle fusion, calcium influx in D1-MSN synapses, together with postsynaptic patch-clamp recordings from SNr neurons, we found that DA inhibits D1-MSN GABA release in a frequency-dependent manner. Surprisingly, this effect was independent of DA receptors and instead required 5-HT1B receptor activation. Using two-photon serotonin biosensor imaging in slices and fiber photometry in vivo, we demonstrate that DA enhances extracellular serotonin in the SNr. Our results suggest that serotonin mediates DAergic control of basal ganglia output and contributes to the therapeutic actions of dopaminergic medications for Parkinson’s disease and psychostimulant-related disorders.
Dataset DOI: 10.5061/dryad.2z34tmpzx
Description of the data and file structure
This dataset contains the source data for all figures.
Each CSV file corresponds to one figure panel as indicated by the filename.
- Columns:
"x" = time or condition
"y" = measurement (e.g., normalized fluorescence, ΔF/F, etc.)
"sem" = standard error of the mean (if applicable)
"n" = number of observations (if applicable)
For details on experimental design, see Materials and Methods in the manuscript.
Contact: Anders Borgkvist, Department of Neuroscience, Karolinska Institutet, anders.borgkvist@ki.se
Files and variables
File: Dataset_Molinari_etal.zip
Root Contents
• README.txt — Text note/README.
Fiber_photometry_analysis_code
• README_FP.txt
– Type: Text file
• ann_5HT_grab.m
– Type: M
Figure1
• Fig1C_source.csv
– Type: CSV (comma-delimited)
– Shape: 198 rows × 5 columns
– Variables: Time_s, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• Fig1D_source.csv
– Type: CSV (comma-delimited)
– Shape: 39 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, FractionalRelease
– Notes: Summary/condition table with mean, SEM, N.
• Fig1E_source.csv
– Type: CSV (comma-delimited)
– Shape: 39 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, BoutonsPerSlice
– Notes: Summary/condition table with mean, SEM, N.
• Fig1F_source.csv
– Type: CSV (comma-delimited)
– Shape: 198 rows × 5 columns
– Variables: Time_s, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• Fig1G_source.csv
– Type: CSV (comma-delimited)
– Shape: 12 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, FractionalRelease
– Notes: Summary/condition table with mean, SEM, N.
Figure2
• Fig2A_source.csv
– Type: CSV (comma-delimited)
– Shape: 264 rows × 5 columns
– Variables: Time_s, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• Fig2B_source.csv
– Type: CSV (comma-delimited)
– Shape: 39 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, FractionalRelease
– Notes: Summary/condition table with mean, SEM, N.
• Fig2C_source.csv
– Type: CSV (comma-delimited)
– Shape: 198 rows × 5 columns
– Variables: Time_s, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• Fig2D_source.csv
– Type: CSV (comma-delimited)
– Shape: 26 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, FractionalRelease
– Notes: Summary/condition table with mean, SEM, N.
• Fig2F_source.csv
– Type: CSV (comma-delimited)
– Shape: 41 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, FractionalRelease
– Notes: Summary/condition table with mean, SEM, N.
Figure3
• Fig3C_source.csv
– Type: CSV (comma-delimited)
– Shape: 278 rows × 4 columns
– Variables: avgDA, semDA, avgCtr, semCtr
– Notes: Tabular dataset.
• Fig3D_source.csv
– Type: CSV (comma-delimited)
– Shape: 4 rows × 4 columns
– Variables: diffDA, diffSKF, semSKF, semDA
– Notes: Tabular dataset.
Figure4
• Fig4G_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 50 rows × 5 columns
– Variables: Time_m, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• Fig4H_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 40 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, oIPSC
– Notes: Summary/condition table with mean, SEM, N.
• Fig4I_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 35 rows × 8 columns
– Variables: RowType, Condition, Mean, SEM, N, PPR value, Unnamed: 6, Unnamed: 7
– Notes: Summary/condition table with mean, SEM, N.
• Fig4K_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 17 rows × 9 columns
– Variables: RowType, Condition, Mean, SEM, N, oIPSC, Unnamed: 6, Unnamed: 7, Unnamed: 8
– Notes: Summary/condition table with mean, SEM, N.
Figure5
• Fig5C_source .csv
– Type: CSV (semicolon-delimited)
– Shape: 30 rows × 5 columns
– Variables: Time_m, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• Fig5D_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 38 rows × 12 columns
– Variables: RowType, Condition, Mean, SEM, N, oIPSC, Unnamed: 6, Unnamed: 7, Unnamed: 8, Unnamed: 9, Unnamed: 10, Unnamed: 11
– Notes: Summary/condition table with mean, SEM, N.
• Fig5E_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 30 rows × 5 columns
– Variables: Time_m, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• Fig5F_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 37 rows × 12 columns
– Variables: RowType, Condition, Mean, SEM, N, oIPSC, Unnamed: 6, Unnamed: 7, Unnamed: 8, Unnamed: 9, Unnamed: 10, Unnamed: 11
– Notes: Summary/condition table with mean, SEM, N.
• Fig5G_source.csv
– Type: CSV (comma-delimited)
– Shape: 278 rows × 6 columns
– Variables: avgDA, semDA, avgCtr, semCtr, avgNAS_DA, semNAS_DA
– Notes: Tabular dataset.
• Fig5H_source.csv
– Type: CSV (comma-delimited)
– Shape: 4 rows × 5 columns
– Variables: semNAS_DA, semDA, diffNAS_DA, diffDA, freq
– Notes: Tabular dataset.
Figure6
• Fig6C_source.csv
– Type: CSV (comma-delimited)
– Shape: 600 rows × 9 columns
– Variables: xVal, avg1micro, sem1micro, xVal.1, mean_sec, sem_sec, xVal.2, avg100nM, sem100nM
– Notes: Tabular dataset.
• Fig6D_source.csv
– Type: CSV (comma-delimited)
– Shape: 35 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, g5HT3_DeltaF_over_F_percent
– Notes: Summary/condition table with mean, SEM, N.
• Fig6E_source.csv
– Type: CSV (comma-delimited)
– Shape: 16 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, g5HT3_DeltaF_over_F_percent
– Notes: Summary/condition table with mean, SEM, N.
• Fig6F_source.csv
– Type: CSV (comma-delimited)
– Shape: 14 rows × 9 columns
– Variables: RowType, Condition, Value, Value_display, Mean, SEM, N, Mean_display, SEM_display
– Notes: Summary/condition table with mean, SEM, N.
Figure7
• Fig7A_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 21 rows × 9 columns
– Variables: time (min), DA 10 µM, sem_DA, time (min).1, Cit 10 µM, sem_Cit, time (min).2, Cit+DA, sem_Cit+DA
– Notes: Tabular dataset.
• Fig7B_source.csv
– Type: CSV (comma-delimited)
– Shape: 49 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, g5HT3_DeltaF_over_F_percent
– Notes: Summary/condition table with mean, SEM, N.
• Fig7D_source.csv
– Type: CSV (comma-delimited)
– Shape: 162 rows × 8 columns
– Variables: DA10_t_avg, DA10_t_sem, DA50_t_avg, DA50_t_sem, aCSF_t_avg, aCSF_t_sem, TBZ_t_avg, TBZ_t_sem
– Notes: Tabular dataset.
• Fig7E_source.csv
– Type: CSV (comma-delimited)
– Shape: 24 rows × 5 columns
– Variables: Time_min, Condition, Mean, SEM, N
– Notes: Tabular dataset.
• Fig7F_source.csv
– Type: CSV (comma-delimited)
– Shape: 21 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, g5HT3_DeltaF_over_F_percent
– Notes: Summary/condition table with mean, SEM, N.
Figure8
• Fig8B_source.csv
– Type: CSV (comma-delimited)
– Shape: 21 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, g5HT3_DeltaF_over_F_percent
– Notes: Summary/condition table with mean, SEM, N.
• Fig8D_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 42 rows × 12 columns
– Variables: RowType, Condition, Mean, SEM, N, light-evoked eIPSC change (%), Unnamed: 6, Unnamed: 7, Unnamed: 8, Unnamed: 9, Unnamed: 10, Unnamed: 11
– Notes: Summary/condition table with mean, SEM, N.
Figure9
• Fig9C_source .csv
– Type: CSV (semicolon-delimited)
– Shape: 37 rows × 12 columns
– Variables: RowType, Condition, Mean, SEM, N, oIPSC, Unnamed: 6, Unnamed: 7, Unnamed: 8, Unnamed: 9, Unnamed: 10, Unnamed: 11
– Notes: Summary/condition table with mean, SEM, N.
• Fig9D_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 37 rows × 13 columns
– Variables: RowType, Condition, Mean, SEM, N, oIPSC, Unnamed: 6, Unnamed: 7, Unnamed: 8, Unnamed: 9, Unnamed: 10, Unnamed: 11, Unnamed: 12
– Notes: Summary/condition table with mean, SEM, N.
• Fig9E_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 37 rows × 13 columns
– Variables: RowType, Condition, Mean, SEM, N, PPR value, Unnamed: 6, Unnamed: 7, Unnamed: 8, Unnamed: 9, Unnamed: 10, Unnamed: 11, Unnamed: 12
– Notes: Summary/condition table with mean, SEM, N.
• Fig9G_source.csv
– Type: CSV (comma-delimited)
– Shape: 276 rows × 4 columns
– Variables: ctr_sem, avg_ctr, avg_cp, cp_sem
– Notes: Tabular dataset.
• Fig9H_source.csv
– Type: CSV (comma-delimited)
– Shape: 4 rows × 3 columns
– Variables: freq, FluoDecr, FluoDecrSem
– Notes: Tabular dataset.
Figure10
• Fig10C_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 433330 rows × 18 columns
– Variables: Time (mins), Vehicle, Unnamed: 2, Unnamed: 3, Unnamed: 4, Unnamed: 5, Unnamed: 6, GBR, Unnamed: 8, Unnamed: 9, Unnamed: 10, Unnamed: 11, Unnamed: 12, Citalopram, Unnamed: 14, Unnamed: 15, Unnamed: 16, Unnamed: 17
– Notes: Tabular dataset.
• Fig10D_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 37 rows × 13 columns
– Variables: RowType, Condition, Mean, SEM, N, zF, Unnamed: 6, Unnamed: 7, Unnamed: 8, Unnamed: 9, Unnamed: 10, Unnamed: 11, Unnamed: 12
– Notes: Summary/condition table with mean, SEM, N.
FigureS1
• FigS1A_source.csv
– Type: CSV (comma-delimited)
– Shape: 132 rows × 5 columns
– Variables: Time_s, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• FigS1B_source.csv
– Type: CSV (comma-delimited)
– Shape: 21 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, FractionalRelease
– Notes: Summary/condition table with mean, SEM, N.
• FigS1C_source.csv
– Type: CSV (comma-delimited)
– Shape: 18 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, g5HT3_DeltaF_over_F_percent
– Notes: Summary/condition table with mean, SEM, N.
FigureS2
• FigS2B.csv
– Type: CSV (comma-delimited)
– Shape: 59 rows × 5 columns
– Variables: Time_s, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• FigS2C.csv
– Type: CSV (comma-delimited)
– Shape: 16 rows × 7 columns
– Variables: RowType, Condition, Mean, SEM, N, FiringFrequency_Hz, PairID
– Notes: Summary/condition table with mean, SEM, N.
• FigS2D.csv
– Type: CSV (comma-delimited)
– Shape: 132 rows × 5 columns
– Variables: Time_s, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• FigS2E.csv
– Type: CSV (comma-delimited)
– Shape: 132 rows × 5 columns
– Variables: Time_s, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• FigS2F.csv
– Type: CSV (comma-delimited)
– Shape: 132 rows × 5 columns
– Variables: Time_s, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• FigS2G.csv
– Type: CSV (comma-delimited)
– Shape: 132 rows × 5 columns
– Variables: Time_s, Condition, Mean, SEM, N
– Notes: Time-course dataset with condition averages and SEM.
• FigS2H.csv
– Type: CSV (comma-delimited)
– Shape: 10 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, FractionalRelease
– Notes: Summary/condition table with mean, SEM, N.
• FigS2I.csv
– Type: CSV (comma-delimited)
– Shape: 10 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, FractionalRelease
– Notes: Summary/condition table with mean, SEM, N.
• FigS2J.csv
– Type: CSV (comma-delimited)
– Shape: 12 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, FractionalRelease
– Notes: Summary/condition table with mean, SEM, N.
• FigS2K.csv
– Type: CSV (comma-delimited)
– Shape: 18 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, FractionalRelease
– Notes: Summary/condition table with mean, SEM, N.
FigureS3
• FigS3B.csv
– Type: CSV (comma-delimited)
– Shape: 32 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, GRAB_DA_dFF_percent_of_baseline
– Notes: Summary/condition table with mean, SEM, N.
• FigS3E.csv
– Type: CSV (comma-delimited)
– Shape: 161 rows × 6 columns
– Variables: avg_nostim, sem_nostim, avg_2hz, sem_2hz, avg_10hz, sem_10hz
– Notes: Tabular dataset.
• FigS3F.csv
– Type: CSV (comma-delimited)
– Shape: 31 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, GRAB_DA_dFF_percent_of_baseline
– Notes: Summary/condition table with mean, SEM, N.
• FigS3G.csv
– Type: CSV (comma-delimited)
– Shape: 161 rows × 6 columns
– Variables: avg_nostim, sem_nostim, avg_2hz, sem_2hz, avg_10hz, sem_10hz
– Notes: Tabular dataset.
• FigS3H.csv
– Type: CSV (comma-delimited)
– Shape: 24 rows × 6 columns
– Variables: RowType, Condition, Mean, SEM, N, GRAB_DA_dFF_percent_of_baseline
– Notes: Summary/condition table with mean, SEM, N.
FigureS4
• FigS4A_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 29 rows × 9 columns
– Variables: RowType, Condition, Mean, SEM, N, PPR value, Unnamed: 6, Unnamed: 7, Unnamed: 8
– Notes: Summary/condition table with mean, SEM, N.
• FigS4B_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 29 rows × 10 columns
– Variables: RowType, Condition, Mean, SEM, N, PPR value, Unnamed: 6, Unnamed: 7, Unnamed: 8, Unnamed: 9
– Notes: Summary/condition table with mean, SEM, N.
• FigS4C_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 31 rows × 9 columns
– Variables: RowType, Condition, Mean, SEM, N, PPR value, Unnamed: 6, Unnamed: 7, Unnamed: 8
– Notes: Summary/condition table with mean, SEM, N.
• FigS4D_source.csv
– Type: CSV (semicolon-delimited)
– Shape: 29 rows × 9 columns
– Variables: RowType, Condition, Mean, SEM, N, PPR value, Unnamed: 6, Unnamed: 7, Unnamed: 8
– Notes: Summary/condition table with mean, SEM, N.