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Long-term raw color image stacks of ring-confined Physarum polycephalum for extraction of contractile activity: Part 1

Saiseau, Raphael12; Busson, Valentin2; Durand, Marc2

Published May 04, 2026 on Dryad. https://doi.org/10.5061/dryad.2z34tmq08

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Abstract

This deposit contains the first half of a raw-image dataset comprising long-term color image stacks of Physarum polycephalum confined in annular (ring) geometries. Images were acquired as time series (image stacks) that capture the evolution of contractile activity and network reorganisation over extended durations (a few-second frame rate for tens of hours). Contractile dynamics are visible only indirectly in the raw frames and require standard image-processing and time-series analysis to extract quantitative measures (local contraction amplitude, phase, wave direction, vein diameter, etc.). The dataset is intended for reuse in studies of large-scale self-organisation and transport in biological networks, and as a benchmark for image-processing, segmentation, tracking, and time-series analysis methods.