https://doi.org/10.5061/dryad.2z34tmq18

Data Records

The dataset comprises four primary MATLAB data structures (patch_s3, optogenetics, perfusion_s1c, and electrical_stimultation), together with two Excel data files associated with Fig. 3 and Supplementary Fig. 4. All datasets follow a nested experimental design in which slices are nested within mice. In MATLAB files all fluorescence traces are sampled at 31 Hz, and a shared variable (time) provides a common time vector.

patch_s3
The “patch_s3.mat”  dataset contains electrophysiological recordings from multiple mice and cells. Each row in the struct represents one slice recorded across multiple experimental conditions, including c2o, cciv_on, cciv_off, and led. The c2o condition reports the onset of 920 nm laser scanning following scan initiation. The cciv_on and cciv_off conditions correspond to standard current-clamp voltage (current) protocols performed with scanning on or off, respectively. The led condition reports LED stimulation during spiking activity recording.
Units: ephys data collected in pA where time for ephys and laser time in seconds.

Each condition-specific sub-struct contains spiking activity data and a corresponding time vector. In the c2o condition, laser onset is additionally reported as a vector aligned to the same time base. In the led condition, LED stimulation consistently occurs at 3 s.

optogenetic
The “optogenetic.mat” dataset consists of multiple struct files, each corresponding to a distinct optogenetic experiment. Each struct includes the fields date (experiment date, equal to the number of mice), slice (slice identifier, nested design), and trace (averaged fluorescence signal, arbitrary units). When applicable, additional fields denote pharmacological or experimental conditions (e.g., mec, citalopram), using the same structural format.

These datasets are mapped to figures as follows: c57 (Supplementary Fig. 1d,e), chrimson (Supplementary Fig. 4), citalopram (Supplementary Fig. 2c–e), cocktail (Fig. 2e–g), dorsal1p (Fig. 2d), ventral1p (Supplementary Fig. 5), idle (Supplementary Fig. 2f–h), mec (Fig. 2b,c), rs (Supplementary Fig. 2a,b), and th_chr2 (Supplementary Fig. 6). A shared variable, time, measured in seconds, provides a general time vector at 31 Hz across all optogenetic measurements.

perfusion_s1c
The “perfusion_s1c.mat”  dataset contains two structs corresponding to bath perfusion of either 10 µM dopamine (DA) or 5-hydroxytryptamine (5-HT). Each struct includes trace, representing averaged fluorescence vectors acquired at different intervals, arbitrary units; time, providing timestamped time vectors for each recording epoch [date type]; file, denoting the slice identifier and nested design; and param, containing experimental and acquisition parameters.

electrical_stimultation
The “electric_stimulation.mat”  dataset contains recordings from multiple mice and cells. The variable electrical_decay (Fig. 1b,c,e,f) includes the fields date (measurement date, equal to the number of mice), slice (slice identifier, nested design), trace (mean fluorescence signal evoked by stimulation [arbitrary units]), and trace_treatment (mean evoked fluorescence [arbitrary units] following application of 10 µM mec), region (segregating experiments done at the dorsal/ ventral striatum).
The variable estim_freq (Supplementary Fig. 8) contains one row per slice across multiple experiments, organized by stimulation frequency and experimental condition (with or without 10 µM mec).
The variable propagation (Fig. 1g,h) includes date and slice identifiers, along with control and mec fields that report fluorescence responses [arbitrary units] as a function of distance from the stimulation origin.
A shared variable, time [sec], provides a general time vector at 31 Hz.

Sapap3_ko data files
Two Excel source data files provide numerical values underlying main and supplementary figures.

NatComm_Fig3_Data_updated013026.xlsx contains raw data for Fig. 3. Fluorescence traces are reported for recordings performed under control artificial cerebrospinal fluid (acsf) conditions and following application of mec. The dataset includes recordings from both control mice and sapap3 knockout (KO) mice, enabling direct comparison between pharmacological and genetic conditions within the same experimental framework.

NatComm_FigS4_Data.xlsx contains data for Supplementary Fig. 7 and follows the same organizational structure. Traces are provided for acsf and mec conditions and include recordings from sapap3 KO mice alongside control data.

In both files, data is presented as Δf/ F0 and are organized in time-aligned [seconds] tabular format. Rows correspond to individual slices or recordings, and columns correspond to time points or experimental conditions, as indicated in the worksheet headers. Grouping by genotype and pharmacological condition matches the figure panels presented in the manuscript.