Data from: Neuronal regulation of myenteric interstitial cells of Cajal (ICC-MY) in the colon
Data files
Jul 21, 2025 version files 22.72 MB
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Bay58.zip
3.19 MB
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CCH.zip
718.12 KB
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MEN-10376.zip
1.20 MB
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Neostigmine.zip
4.80 MB
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Nononate.zip
2.91 MB
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ODQ.zip
1.91 MB
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README.md
2.46 KB
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RP-67580.zip
2.36 MB
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SubstanceP.zip
1.28 MB
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TTX.zip
4.33 MB
Abstract
Interstitial cells of Cajal (ICC) generate contractile patterns of colonic motility. We investigated innervation of ICC within the plane of the myenteric plexus (ICC-MY) in proximal colon using mice expressing GCaMP6f in ICC. ICC-MY generated localized Ca2+ transients that couple to activation of ANO1 channels, a Ca2+-activated Cl- conductance. ICC are electrically coupled to SMCs, so activation or suppression of currents in ICC affects excitability of SMCs. ICC-MY displayed tonic inhibition, as the neurotoxin, TTX, increased the frequency of Ca2+ transients. Tonic inhibition was mimicked by a nitric oxide donor, NONOate, and by a guanylate cyclase agonist (Bay 58-2667). In contrast, ODQ mimicked the effects of TTX, increasing Ca2+ transients. Carbachol (CCh) increased Ca2+ transients in ICC-MY, and these effects were mediated by M3 muscarinic receptors. Neostigmine also increased Ca2+ transients, suggesting there is tonic activation of enteric excitatory neurons in colonic muscles. Substance P and antagonists of NK1 and NK2 receptors had no effect on Ca2+ transients in ICC-MY. Electrical field stimulation (EFS), under conditions that emphasized excitatory neural responses, enhanced Ca2+ transients, and these effects were blocked by atropine or an M3 receptor antagonist (DAU 5884). EFS in the presence of atropine caused inhibition of Ca2+ via release of NO. Cessation of nitrergic stimulation resulted in a substantial increase in Ca2+ transients, known as post-stimulus excitation. In summary, ICC-MY, important for the generation of propulsive contractions in the colon, are innervated by excitatory (cholinergic) and inhibitory (nitrergic) motor neurons, and these inputs regulate the excitability of these cells.
Dataset DOI: 10.5061/dryad.31zcrjf0k
Description of the data and file structure
The file includes image datasets from ICC-MY cellular calcium events under control and pharmacological agents.
This directory contains subfolders related to different compounds used in experiments. Each folder contains data corresponding to a specific control subfolder and treatment subfolder. Below is a brief description of each subfolder:
Files and variables
File: Bay58.zip
Description: Contains experimental data related to Bay-58-2667 and its effects on cellular activity.
2 subfolders:
control and in the presence of Bay-58-2667
File: CCH.zip
Description: Includes data for Carbachol (CCH) affecting calcium signaling in ICC-MY.
2 subfolders:
Control and in the presence of CCH
File: MEN-10376.zip
Description: Contains data for experiments using MEN-10376 and its effects on ICC-MY calcium transients.
2 subfolders:
Control and in the presence of MEN-10376
File: Neostigmine.zip
Description: Stores experimental results related to Neostigmine on ICC-MY calcium signaling.
2 subfolders:
Control and in the presence of Neostigmine
File: Nononate.zip
Description: A subset of Nononate experiments effects on ICC-MY calcium signals.
2 subfolders: control and in the presence of Nononate
File: ODQ.zip
Description: Contains data related to ODQ, effects on ICC-MY calcium signals.
2 subfolders: control and in the presence of ODQ
File: RP-67580.zip
Description: Includes experimental data involving RP-67580, effects on ICC-MY calcium dynamics.
2 subfolders: control and in the presence of RP-67580
File: SubstanceP.zip
Description: Contains results from experiments with Substance P, effects on ICC-MY calcium homeostasis.
2 subfolders: control and in the presence of Substance P
File: TTX.zip
Description: Contains results from experiments with TTX, effects on ICC-MY calcium transients.
2 subfolders: control and in the presence of Substance P
Code/software
- Fiji/ImageJ (National Institutes of Health, MD, USA, http://rsbweb.nih.gov/ij).
- STMapAuto software: https://github.com/gdelvalle99/STMapAuto