Data from: A microbiome-derived nucleobase underlies tyrosine metabolism and predator avoidance in mosquito larvae
Data files
Jun 09, 2026 version files 98.96 MB
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Fig._S11.zip
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Fig._S12.zip
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Fig._S13.zip
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Figure_1.zip
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Figure_2.zip
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Figure_3.zip
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Figure_4.zip
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Figure_S10.zip
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Figure_S2.zip
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Figure_S3.zip
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Figure_S5.zip
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Figure_S6.zip
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Figure_S8.zip
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Figure_S9.zip
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README.md
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Table_S1.zip
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Table_S2.zip
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Table_S3.zip
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Abstract
This dataset supports a study investigating how the microbiome-derived nucleobase queuosine (Q) influences mosquito larval physiology, metabolism, and predator avoidance behavior. It includes RNA-seq data, metabolite measurements (tyrosine, dopamine, and BH4), tRNA modification profiles, and behavioral and predation assay outputs comparing larvae with Q-producing and Q-deficient microbiomes. The data demonstrate that loss of microbiome-derived Q disrupts tyrosine metabolism and downstream processes, affecting cuticle formation, locomotion, and ecological interactions. Supplementation experiments with queuine and tyrosine are also included, showing partial or full recovery of molecular and phenotypic traits. Together, these datasets provide a comprehensive resource linking microbiome-derived nutrients to molecular regulation, organismal traits, and predator–prey dynamics in mosquito larvae.
All datasets are organized into subfolder based upon the figures and processed according to methods described in the manuscript. If there are questions, please contact Joshua Benoit and Melissa Kelley.
Description of data and file structure: Each dataset is organized by Figure. Files provided under each subheading are for the associated figure.
Figure_1.zip
Files and variables
File: Figure1-qPCRvalidation-queA.csv
Description: Mosquito microbiome composition is evaluated using the DDCT values of expression of the bacterial queA and queE gene.
Variables
- Group: mosquito larvae grown with K12, queA, queA + q, or from laboratory non-sterile conditions.
- QueE: Expression from queE.
- Control: Bacterial control gene
- DCT: delta Ct values.
- DDCT: delta delta Ct values calculated.
- Relative expression: Relative expression values for queA.
File: Figure1-Q-nucleosides.xlsx
Description: Peak areas of extracted ion chromatogram for Q modifications. Peak areas were normalized with the canonical peak areas to give a relative abundance of the modifications in the tRNA sample. Raw data files are listed below.
Variables: Mosquito larvae grown with K12 (control) or queA bacteria.
File: K12_nucleosides_R.csv
Description: The normalized relative abundances for nucleosides in tRNA of K12 larvae generated from LC-MS/MS data (files listed below).
Variables
- K12_L1: nucleosides from larvae grown with K12 bacteria - biological replicate 1.
- K12_L2: nucleosides from larvae grown with K12 bacteria - biological replicate 2.
- K12_L3: nucleosides from larvae grown with K12 bacteria - biological replicate 3.
File: 7_K12larva_1.raw
Description: LC-MS/MS raw data file of nucleoside analysis in tRNA of K12 larvae (biological replicate 1). LC-MS/MS data is processed using Qual Browswer in Xcalibur 3.0. Using the mass filter, each extracted ion chromatogram was isolated for precursor mass and detected mass transitions for modifications. The peak area of the XIC was then utilized from this file and normalized using summation of canonical nucleosides peak areas (C, A, G, U) to calculate the relative abundance.
File: 8_K12larva_2.raw
Description: LC-MS/MS raw data file of nucleoside analysis in tRNA of K12 larvae (biological replicate 2). LC-MS/MS data is processed using Qual Browswer in Xcalibur 3.0. Using the mass filter, each extracted ion chromatogram was isolated for precursor mass and detected mass transitions for modifications. The peak area of the XIC was then utilized from this file and normalized using summation of canonical nucleosides peak areas (C, A, G, U) to calculate the relative abundance.
File: 9_K12larva_3.raw
Description: LC-MS/MS raw data file of nucleoside analysis in tRNA of K12 larvae (biological replicate 3). LC-MS/MS data is processed using Qual Browswer in Xcalibur 3.0. Using the mass filter, each extracted ion chromatogram was isolated for precursor mass and detected mass transitions for modifications. The peak area of the XIC was then utilized from this file and normalized using summation of canonical nucleosides peak areas (C, A, G, U) to calculate the relative abundance.
File: queA_nucleoside_R.csv
Description: The normalized relative abundances for nucleosides in tRNA of queA larvae generated from LC-MS/MS data (files listed below).
Variables
- Q_L1: nucleosides from larvae grown with queA bacteria - biological replicate 1.
- Q_L2: nucleosides from larvae grown with queA bacteria - biological replicate 2.
- Q_L3: nucleosides from larvae grown with queA bacteria - biological replicate 3.
File: 10_queAlarva_1.raw
Description: LC-MS/MS raw data file of nucleoside analysis in tRNA of queA larvae (biological replicate 1). LC-MS/MS data is processed using Qual Browswer in Xcalibur 3.0. Using the mass filter, each extracted ion chromatogram was isolated for precursor mass and detected mass transitions for modifications. The peak area of the XIC was then utilized from this file and normalized using summation of canonical nucleosides peak areas (C, A, G, U) to calculate the relative abundance.
File: 11_queAlarva_2.raw
Description: LC-MS/MS raw data file of nucleoside analysis in tRNA of queA larvae (biological replicate 2). LC-MS/MS data is processed using Qual Browswer in Xcalibur 3.0. Using the mass filter, each extracted ion chromatogram was isolated for precursor mass and detected mass transitions for modifications. The peak area of the XIC was then utilized from this file and normalized using summation of canonical nucleosides peak areas (C, A, G, U) to calculate the relative abundance.
File: 12_queAlarva_3.raw
Description: LC-MS/MS raw data file of nucleoside analysis in tRNA of queA larvae (biological replicate 3). LC-MS/MS data is processed using Qual Browswer in Xcalibur 3.0. Using the mass filter, each extracted ion chromatogram was isolated for precursor mass and detected mass transitions for modifications. The peak area of the XIC was then utilized from this file and normalized using summation of canonical nucleosides peak areas (C, A, G, U) to calculate the relative abundance.
File: WT_nucleoside_R.csv
Description: The normalized relative abundances for nucleosides in tRNA of larvae grown in typical laboratory conditions (WT) generated from LC-MS/MS data (files listed below).
Variables
- Modification: All detected tRNA modifications.
- WT_L1: nucleosides from larvae grown in laboratory insectary - biological replicate 1.
- WT_L2: nucleosides from larvae grown in laboratory insectary - biological replicate 2.
- WT_L3: nucleosides from larvae grown in laboratory insectary - biological replicate 3.
File: 13_WTlarva_1.raw
Description: LC-MS/MS raw data file of nucleoside analysis in tRNA of laboratory (WT) larvae (biological replicate 1). LC-MS/MS data is processed using Qual Browswer in Xcalibur 3.0. Using the mass filter, each extracted ion chromatogram was isolated for precursor mass and detected mass transitions for modifications. The peak area of the XIC was then utilized from this file and normalized using summation of canonical nucleosides peak areas (C, A, G, U) to calculate the relative abundance.
File: 14_WTlarva_2.raw
Description: LC-MS/MS raw data file of nucleoside analysis in tRNA of laboratory (WT) larvae (biological replicate 2). LC-MS/MS data is processed using Qual Browswer in Xcalibur 3.0. Using the mass filter, each extracted ion chromatogram was isolated for precursor mass and detected mass transitions for modifications. The peak area of the XIC was then utilized from this file and normalized using summation of canonical nucleosides peak areas (C, A, G, U) to calculate the relative abundance.
File: 15_WTlarva_3.raw
Description: LC-MS/MS raw data file of nucleoside analysis in tRNA of laboratory (WT) larvae (biological replicate 3). LC-MS/MS data is processed using Qual Browswer in Xcalibur 3.0. Using the mass filter, each extracted ion chromatogram was isolated for precursor mass and detected mass transitions for modifications. The peak area of the XIC was then utilized from this file and normalized using summation of canonical nucleosides peak areas (C, A, G, U) to calculate the relative abundance.
Figure_2.zip
Files and variables
File: Figure2-RNAseq-expression-values.csv
Description: Expression of genes from RNAseq data in larvae.
Variables
- target_id: Genes of interest.
- queA1: reads from RNAseq analysis of queA larvae - biological replicate 1.
- queA2: reads from RNAseq analysis of queA larvae - biological replicate 2.
- queA3: reads from RNAseq analysis of queA larvae - biological replicate 3.
- K12_1: reads from RNAseq analysis of K12 larvae - biological replicate 1.
- K12_2: reads from RNAseq analysis of K12 larvae - biological replicate 2.
- K12_3: reads from RNAseq analysis of K12 larvae - biological replicate 3.
- queA_50_1: reads from RNAseq analysis of queA larvae supplemented with 50uM queuine - biological replicate 1.
- queA_50_2: reads from RNAseq analysis of queA larvae supplemented with 50uM queuine - biological replicate 2.
- queA_50_3: reads from RNAseq analysis of queA larvae supplemented with 50uM queuine - biological replicate 3.
File: Figure2-Tyrosine.csv
Description: Levels of tyrosine in larvae.
Variables
- Rep: Replicate number.
- Treatment: Type of bacteria grown with larvae.
- Tyr: Concentration of tyrosine.
File: Figure_2_-_BH4.csv
Description: Levels of BH4 in larvae.
Variables
- Group: Replicate and type of bacteria.
- Level: Concentration of BH4.
File: Figure2-TyrosinePathway.csv
Description: Expression of transcripts related to the tyrosine metabolism pathway in queA, K12, and queA supplemented with q larvae.
Variables
- Gene: Target gene in Aedes aegypti.
- Gene name: Common name of gene.
- Enzyme: Function of enzyme in pathway.
- queA_1: Expression value for queA larvae replicate 1.
- queA_2: Expression value for queA larvae replicate 2.
- queA_3: Expression value for queA larvae replicate 3.
- K12_1: Expression value for K12 larvae replicate 1.
- K12_2: Expression value for K12 larvae replicate 2.
- K12_3: Expression value for K12 larvae replicate 3.
- queA_50_1: Expression value for queA + q larvae replicate 1.
- queA_50_2: Expression value for queA + q larvae replicate 2.
- queA_50_3: Expression value for queA + q larvae replicate 3.
Figure_3.zip
Files and variables
File: Figure3-grayvalue-measurements-qsupplement.csv
Description: Gray values calculated by using ImageJ to inverese and gray scale the photos files provided below. Individual larvae were imaged and each body part 20 points were collected.
Variables
- Body location: head, thorax, abdomen.
- K12-1: gray values from K12 larva #1.
- K12-2: gray values from K12 larva #2.
- K12-3: gray values from K12 larva #3.
- K12-4: gray values from K12 larva #4.
- K12-5: gray values from K12 larva #5.
- K12-6: gray values from K12 larva #6.
- K12-7: gray values from K12 larva #7.
- K12-8: gray values from K12 larva #8.
- K12-9: gray values from K12 larva #9.
- K12-10: gray values from K12 larva #10.
- K12-11: gray values from K12 larva #11.
- K12-12: gray values from K12 larva #12.
- K12-13: gray values from K12 larva #13.
- K12-14: gray values from K12 larva #14.
- K12-15: gray values from K12 larva #15.
- queA-1: gray values from queA larva #1.
- queA-2: gray values from queA larva #2.
- queA-3: gray values from queA larva #3.
- queA-4: gray values from queA larva #4.
- queA-5: gray values from queA larva #5.
- queA-6: gray values from queA larva #6.
- queA-7: gray values from queA larva #7.
- queA-8: gray values from queA larva #8.
- queA-9: gray values from queA larva #9.
- queA-10: gray values from queA larva #10.
- queA-11: gray values from queA larva #11.
- queA-12: gray values from queA larva #12.
- queA-13: gray values from queA larva #13.
- queA-14: gray values from queA larva #14.
- queA-15: gray values from queA larva #15.
- queA+q-1: gray values from queA + q larva #1.
- queA+q-2: gray values from queA + q larva #2.
- queA+q-3: gray values from queA + q larva #3.
- queA+q-4: gray values from queA + q larva #4.
- queA+q-5: gray values from queA + q larva #5.
- queA+q-6: gray values from queA + q larva #6.
- queA+q-7: gray values from queA + q larva #7.
- queA+q-8: gray values from queA + q larva #8.
- queA+q-9: gray values from queA + q larva #9.
- queA+q-10: gray values from queA + q larva #10.
- queA+q-11: gray values from queA + q larva #11.
- queA+q-12: gray values from queA + q larva #12.
- queA+q-13: gray values from queA + q larva #13.
- queA+q-14: gray values from queA + q larva #14.
- queA+q-15: gray values from queA + q larva #15.
File: K12_L1.jpg
Description: image of K12 larva #1 used for calculating gray value measurements.
File: K12_L2.jpg
Description: image of K12 larva #2 used for calculating gray value measurements.
File: K12_L3.jpg
Description: image of K12 larva #3 used for calculating gray value measurements.
File: K12_L4.jpg
Description: image of K12 larva #4 used for calculating gray value measurements.
File: K12_L5.jpg
Description: image of K12 larva #5 used for calculating gray value measurements.
File: K12_L6.jpg
Description: image of K12 larva #6 used for calculating gray value measurements.
File: K12_L7.jpg
Description: image of K12 larva #7 used for calculating gray value measurements.
File: K12_L8.jpg
Description: image of K12 larva #8 used for calculating gray value measurements.
File: K12_L9.jpg
Description: image of K12 larva #9 used for calculating gray value measurements.
File: K12_L10.jpg
Description: image of K12 larva #10 used for calculating gray value measurements.
File: K12_L11.jpg
Description: image of K12 larva #11 used for calculating gray value measurements.
File: K12_L12.jpg
Description: image of K12 larva #12 used for calculating gray value measurements.
File: K12_L13.jpg
Description: image of K12 larva #13 used for calculating gray value measurements.
File: K12_L14.jpg
Description: image of K12 larva #14 used for calculating gray value measurements.
File: K12_L15.jpg
Description: image of K12 larva #15 used for calculating gray value measurements.
File: queA_L1.jpg
Description: image of queA larva #1 used for calculating gray value measurements.
File: queA_L2.jpg
Description: image of queA larva #1 used for calculating gray value measurements.
File: queA_L3.jpg
Description: image of queA larva #1 used for calculating gray value measurements.
File: queA_L4.jpg
Description: image of queA larva #1 used for calculating gray value measurements.
File: queA_L5.jpg
Description: image of queA larva #1 used for calculating gray value measurements.
File: queA_L6.jpg
Description: image of queA larva #1 used for calculating gray value measurements.
File: queA_L7jpg
Description: image of queA larva #1 used for calculating gray value measurements.
File: queA_L8.jpg
Description: image of queA larva #1 used for calculating gray value measurements.
File: queA_L9.jpg
Description: image of queA larva #1 used for calculating gray value measurements.
File: queA_L10.jpg
Description: image of queA larva #1 used for calculating gray value measurements.
File: queA_L11.jpg
Description: image of queA larva #1 used for calculating gray value measurements.
File: queA_L12.jpg
Description: image of queA larva #1 used for calculating gray value measurements.
File: queA_L13.jpg
Description: image of queA larva #13 used for calculating gray value measurements.
File: queA_L14.jpg
Description: image of queA larva #14 used for calculating gray value measurements.
File: queA_L15.jpg
Description: image of queA larva #15 used for calculating gray value measurements.
File: Figure3-dopamine.csv
Description: Levels of dopamine in larvae.
Variables
- Treatment: queA bacteria or K12 bacteria or queA supplemented with q.
- ng/mL: concentration of dopamine.
- nmol/L: molar concentrations of dopamine per mosquito larvae.
Figure_4.zip
File: Figure4-background-survival-predation.csv
Description: Survival of larvae exposed to predation in different backgrounds (white or black).
Variables
- Larvae: Predator beetle larvae performing the hunting.
- L1-A: survival of queA mosquito larvae from predator larvae 1 hunting.
- L3-A: survival of queA mosquito larvae from predator larvae 3 hunting.
- L4-A: survival of queA mosquito larvae from predator larvae 4 hunting.
- L9-A: survival of queA mosquito larvae from predator larvae 9 hunting.
- L10-A: survival of queA mosquito larvae from predator larvae 10 hunting.
- L11-A: survival of queA mosquito larvae from predator larvae 11 hunting.
- L12-A: survival of queA mosquito larvae from predator larvae 12 hunting.
- L1-B: survival of K12 mosquito larvae from predator larvae 1 hunting.
- L3-B: survival of K12 mosquito larvae from predator larvae 3 hunting.
- L4-B: survival of K12 mosquito larvae from predator larvae 4 hunting.
- L9-B: survival of K12 mosquito larvae from predator larvae 9 hunting.
- L10-B: survival of K12 mosquito larvae from predator larvae 10 hunting.
- L11-B: survival of K12 mosquito larvae from predator larvae 11 hunting.
- L12-B: survival of K12 mosquito larvae from predator larvae 12 hunting.
File: predation-events_survival.csv
Description: Calculated survival based on predation events and predator prey interactions.
Variables
- code: Unique predation event identifier.
- event.no: Sequential predation event number.
- event.time: Time stamp of event.
- pred.notice.time: Time when predator noticed/detected prey.
- pre.strike.st: Pre-strike time stamp used to order predation attempts for cumulative survival curves.
- lar.location.str: Prey/larval spatial grid location before strike.
- pred.suc: Predation outcome; Y = successful capture, N = unsuccessful capture/escape.
- no.surv: Number of larvae surviving after the predation attempt.
- video.label: Unique video identifier used to define replicate videos and clustering.
- tment: Larval treatment group: K12, queA, or * queA+q.
- status: Status/event indicator included in survival dataset.
File: Figure4-qsupp-predation-events.csv
Description: success and misses of predator strike events when hunting mosquito larvae.
Variables
Treatment: type of bacteria and supplementation of q for queA larvae.
- K12-1: success rate of predator strikes for K12 larvae replicate 1.
- K12-2: success rate of predator strikes for K12 larvae replicate 2.
- K12-3: success rate of predator strikes for K12 larvae replicate 3.
- queA-1: success rate of predator strikes for queA larvae replicate 1.
- queA-2: success rate of predator strikes for queA larvae replicate 2.
- queA-3: success rate of predator strikes for queA larvae replicate 3.
- queA + q-1: success rate of predator strikes for queA + q larvae replicate 1.
- queA + q-2: success rate of predator strikes for queA + q larvae replicate 2.
- queA + q-3: success rate of predator strikes for queA + q larvae replicate 3.
File: Figure4-vertical-analyses.csv
Description: Successful captures or misses for predator larvae strikes towards mosquito larvae
Variables
Treatment:
- K12-1: outcomes for K12 mosquito larvae replicate 1
- K12-2: outcomes for K12 mosquito larvae replicate 2
- K12-3: outcomes for K12 mosquito larvae replicate 3
- queA-1: outcomes for queA mosquito larvae replicate 1
- queA-2: outcomes for queA mosquito larvae replicate 2
- queA-3: outcomes for queA mosquito larvae replicate 3
File: predation-events_data_analysis_qsupp-predation.csv
Description: analysis of predation events for changes in predator prey interactions
Variables
- code : Unique predation event identifier.
- event.no: Sequential predation event number within a video.
- event.time: Time stamp of predation event.
- pred.notice.time : Time when predator was scored as noticing/detecting prey.
- pre.strike.st: Pre-strike time stamp used to order predation attempts for cumulative survival curves.
- lar.location.str: Prey/larval spatial grid location before strike.
- pred.location.str: location of predator at strike.
- pred.suc: Predation outcome; Y = successful capture, N = unsuccessful capture/escape.
- strike.seq: Strike sequence category, if present.
- w.burst: Wriggle burst count
- w.count: Total wriggle count
- str.time.diff: Strike-associated timing difference
- notice.pred.pos: Predator position at notice
- notice.prey.pos: Prey position at notice
- handle.time: Handling time point
- handle.dur: Handling duration
- video.label: Unique source video identifier
- tment: Larval treatment group: K12, queA, or * queA+q.
- track.dur: Total scored tracking duration for the activity observation.
- block : Experimental block identifier.
File: baselineactivity_data_analysis_v1.csv
Description: analysis of baseline activity for mosquito larvae grown with different levels of Q.
Variables
- code : Unique predation event identifier.
- event.no: Sequential predation event number within a video.
- event.time.st: Start time of scored baseline behavioral event.
- event.time.end : End time of scored baseline behavioral event.
- larvae.no: Larva number within the scored event or video.
- activity.type: Activity category: browsing, filtering, resting, or wriggling.
- time : Time spent in the scored activity category.
- video.label: Unique source video identifier.
- tment : Larval treatment group: K12, queA, or * queA+q.
- track.dur: Total scored tracking duration for the activity observation.
- video.id: Video replicate or recording identifier.
- video.time.skip: Video time interval skipped before scoring, such as 5min.
File: baselinedata_data_analysis_v1.csv
Description: baseline data used for baseline analysis of mosquito larvae movements
Variables
- code : Unique larval/track identifier.
- event.no: Sequential scored event number.
- event.time.st: Start time of scored baseline activity interval.
- event.time.end : End time of scored baseline activity interval.
- start.pos: Starting spatial position of the larva for the scored baseline event.
- w.burst : Number of discrete wriggle burst events.
- w.count : Total wriggling counts. Header has trailing space.
- video.label: Unique video identifier used to define replicate videos and clustering.
- tment : Larval treatment group: K12, queA, or * queA+q.
- track.dur : Duration of behavioral tracking interval, used as model offset.
- video.id : Video replicate or recording identifier.
- video.time.skip : Video time interval skipped before scoring, such as 2min.
File: point.data.csv
Description: Imported row index from original data export.
Variables
- Video: label for videos
- code: Unique predation event identifier.
- event.no: Sequential predation event number.
- event.time: Time stamp of event.
- frame.notice: Video frame number when predator noticed/detected prey.
- frame.strike: Video frame number at predator strike.
- pred.notice.time: Time when predator noticed/detected prey.
- pred.strike.st: Time of predator strike.
- lar.location.str: Prey/larval spatial grid location before strike.
- pred.suc: Predation outcome; Y = successful capture, N = unsuccessful capture/escape.
- strike.seq: Strike sequence category, if present.
- w.burst: Wriggle burst count
- w.count: Total wriggle count
- str.time.diff: Strike-associated timing difference
- notice.pred.pos: Predator position at notice
- notice.prey.pos: Prey position at notice
- handle.time: Handling time point
- handle.dur: Handling duration
- video.label: Unique source video identifier
- tment: Larval treatment group: K12, queA, or * queA+q.
- t.diff: Elapsed time from prey detection to predator strike, in seconds.
- Di.L: Larval/prey movement distance or displacement metric used to compute velocity
- Di.P: Predator movement distance or displacement metric used to compute velocity
- Di.LP: Distance between larva/prey and predator at prey detection/approach.
- Di.LP.norm: Normalized predator-prey distance.
- vel.L: Larval/prey velocity estimate.
- vel.P: Predator velocity estimate.
- frame.diff: Difference between notice frame and strike frame.
File: prey.pos_queA_q.csv
Description: Grid row label for arena position matrix (predator, queA + q).
Variables
- Replicate: Column labels of matrix (A-L)
- 1: Counts/frequencies of predator occurrence at arena grid cell 1 for queA + q.
- 2: Counts/frequencies of predator occurrence at arena grid cell 2 for queA + q.
- 3: Counts/frequencies of predator occurrence at arena grid cell 3 for queA + q.
- 4: Counts/frequencies of predator occurrence at arena grid cell 4 for queA + q.
- 5: Counts/frequencies of predator occurrence at arena grid cell 5 for queA + q.
- 6: Counts/frequencies of predator occurrence at arena grid cell 6 for queA + q.
- 7: Counts/frequencies of predator occurrence at arena grid cell 7 for queA + q.
- 8: Counts/frequencies of predator occurrence at arena grid cell 8 for queA + q.
- 9: Counts/frequencies of predator occurrence at arena grid cell 9 for queA + q.
- 10: Counts/frequencies of predator occurrence at arena grid cell 10 for queA + q.
- 11: Counts/frequencies of predator occurrence at arena grid cell 11 for queA + q.
- 12: Counts/frequencies of predator occurrence at arena grid cell 12 for queA + q.
- 13: Counts/frequencies of predator occurrence at arena grid cell 13 for queA + q.
- 14: Counts/frequencies of predator occurrence at arena grid cell 14 for queA + q.
- 15: Counts/frequencies of predator occurrence at arena grid cell 15 for queA + q.
- 16: Counts/frequencies of predator occurrence at arena grid cell 16 for queA + q.
- 17: Counts/frequencies of predator occurrence at arena grid cell 17 for queA + q.
- 18: Counts/frequencies of predator occurrence at arena grid cell 18 for queA + q.
- 19: Counts/frequencies of predator occurrence at arena grid cell 19 for queA + q.
- 20: Counts/frequencies of predator occurrence at arena grid cell 20 for queA + q.
- 21: Counts/frequencies of predator occurrence at arena grid cell 21 for queA + q.
File: prey.pos_queA.csv
Description: Grid row label for arena position matrix (predator, queA).
Variables
- Replicate: Column labels of matrix (A-L)
- 1: Counts/frequencies of predator occurrence at arena grid cell 1 for queA.
- 2: Counts/frequencies of predator occurrence at arena grid cell 2 for queA.
- 3: Counts/frequencies of predator occurrence at arena grid cell 3 for queA.
- 4: Counts/frequencies of predator occurrence at arena grid cell 4 for queA.
- 5: Counts/frequencies of predator occurrence at arena grid cell 5 for queA.
- 6: Counts/frequencies of predator occurrence at arena grid cell 6 for queA.
- 7: Counts/frequencies of predator occurrence at arena grid cell 7 for queA.
- 8: Counts/frequencies of predator occurrence at arena grid cell 8 for queA.
- 9: Counts/frequencies of predator occurrence at arena grid cell 9 for queA.
- 10: Counts/frequencies of predator occurrence at arena grid cell 10 for queA.
- 11: Counts/frequencies of predator occurrence at arena grid cell 11 for queA.
- 12: Counts/frequencies of predator occurrence at arena grid cell 12 for queA.
- 13: Counts/frequencies of predator occurrence at arena grid cell 13 for queA.
- 14: Counts/frequencies of predator occurrence at arena grid cell 14 for queA.
- 15: Counts/frequencies of predator occurrence at arena grid cell 15 for queA.
- 16: Counts/frequencies of predator occurrence at arena grid cell 16 for queA.
- 17: Counts/frequencies of predator occurrence at arena grid cell 17 for queA.
- 18: Counts/frequencies of predator occurrence at arena grid cell 18 for queA.
- 19: Counts/frequencies of predator occurrence at arena grid cell 19 for queA.
- 20: Counts/frequencies of predator occurrence at arena grid cell 20 for queA.
- 21: Counts/frequencies of predator occurrence at arena grid cell 21 for queA.
File: prey.pos_K12.csv
Description: Grid row label for arena position matrix (predator, K12).
Variables
- Replicate: Column labels of matrix (A-L)
- 1: Counts/frequencies of predator occurrence at arena grid cell 1 for K12.
- 2: Counts/frequencies of predator occurrence at arena grid cell 2 for K12.
- 3: Counts/frequencies of predator occurrence at arena grid cell 3 for K12.
- 4: Counts/frequencies of predator occurrence at arena grid cell 4 for K12.
- 5: Counts/frequencies of predator occurrence at arena grid cell 5 for K12.
- 6: Counts/frequencies of predator occurrence at arena grid cell 6 for K12.
- 7: Counts/frequencies of predator occurrence at arena grid cell 7 for K12.
- 8: Counts/frequencies of predator occurrence at arena grid cell 8 for K12.
- 9: Counts/frequencies of predator occurrence at arena grid cell 9 for K12.
- 10: Counts/frequencies of predator occurrence at arena grid cell 10 for K12.
- 11: Counts/frequencies of predator occurrence at arena grid cell 11 for K12.
- 12: Counts/frequencies of predator occurrence at arena grid cell 12 for K12.
- 13: Counts/frequencies of predator occurrence at arena grid cell 13 for K12.
- 14: Counts/frequencies of predator occurrence at arena grid cell 14 for K12.
- 15: Counts/frequencies of predator occurrence at arena grid cell 15 for K12.
- 16: Counts/frequencies of predator occurrence at arena grid cell 16 for K12.
- 17: Counts/frequencies of predator occurrence at arena grid cell 17 for K12.
- 18: Counts/frequencies of predator occurrence at arena grid cell 18 for K12.
- 19: Counts/frequencies of predator occurrence at arena grid cell 19 for K12.
- 20: Counts/frequencies of predator occurrence at arena grid cell 20 for K12.
- 21: Counts/frequencies of predator occurrence at arena grid cell 21 for K12.
Supplemental Information Files
Figure S1
File: Figure1-qPCRvalidation-queA.csv
Description: Mosquito microbiome composition is evaluated using the DDCT values of expression of the bacterial queA and queE gene.
Variables: Larvae are grown with either K12 or queA bacteria. All other conditions for growth were the same.
- Group: mosquito larvae grown with K12, queA, queA + q, or from laboratory non-sterile conditions.
- QueE: Expression from queE.
- Control: Control gene expression
- DCT: delta Ct values.
- DDCT: delta delta Ct values calculated.
- Relative expression: Relative expression values for queA.
Figure_S2.zip
File: FigureS2-Q-nucleosides.csv
Description: Relative abundance of Q nucleosides from LC-MS/MS.
Variables
- Q: values are for Q modifications oQ, Q, manQ.
- K12_L1: Abundances for K12 replicate 1.
- K12_L2: Abundances for K12 replicate 2.
- K12_L3: Abundances for K12 replicate 3.
- Q_L1: Abundances for queA replicate 1.
- Q_L2: Abundances for queA replicate 2.
- Q_L3: Abundances for queA replicate 3.
- WT_L1: Abundances for WT replicate 1.
- WT_L2: Abundances for WT replicate 2.
- WT_L3: Abundances for WT replicate 3.
Figure_S3.zip
File: FigureS3-growthstage-data.csv
Description: Number of mosquitoes in each growth stage to evaluate development rate.
Variables
- Treatment: Larvae grown with either queA or K12 bacteria.
- Day: Day of measurement.
- Larva: Number of larvae.
- Pupa: Number of pupa.
- Adult: Number of adults.
Table_S1.zip
File: TableS1-relativeabundance-modifications.xlsx
Description: Table of the relative abundance for all modifications and p-value comparison.
Figure_S5.zip
File: K12_larva_1.mzML
Description: Oligonucleotide LC-MS/MS file for mapping tRNA modifications of K12 larvae, replicate 1. Data analysis was performed using RNAModMapper (RAMM) to the mature tRNA sequences with specific interest in the anticodon region of predicted Q-containing tRNAs (Asp-GTC, Tyr-GTA, His-GTG, and Asn-GTT). Monoisotopic mass was selected using 0.6 and 0.1 as the percursor ion tolerance and product ion tolerance, respectively. The P-score cutoff was 55 and dot product of 0.65 for confidence. All hits were then manually confirmed by identifying 70% of c/y ions and 30% for a-B/y ions.
File: Aaegypti-maturetRNA-2-GUNonly.fasta
Description: mature tRNA sequences for tRNAs that may contain Q at position 34.
File: K12_larva_2.mzML
Description: Oligonucleotide LC-MS/MS file for mapping tRNA modifications of K12 larvae, replicate 2. Data analysis was performed using RNAModMapper (RAMM) to the mature tRNA sequences with specific interest in the anticodon region of predicted Q-containing tRNAs (Asp-GTC, Tyr-GTA, His-GTG, and Asn-GTT). Monoisotopic mass was selected using 0.6 and 0.1 as the percursor ion tolerance and product ion tolerance, respectively. The P-score cutoff was 55 and dot product of 0.65 for confidence. All hits were then manually confirmed by identifying 70% of c/y ions and 30% for a-B/y ions.
File: queA_larva_1.mzML
Description: Oligonucleotide LC-MS/MS file for mapping tRNA modifications of queA larvae, replicate 1. Data analysis was performed using RNAModMapper (RAMM) to the mature tRNA sequences with specific interest in the anticodon region of predicted Q-containing tRNAs (Asp-GTC, Tyr-GTA, His-GTG, and Asn-GTT). Monoisotopic mass was selected using 0.6 and 0.1 as the percursor ion tolerance and product ion tolerance, respectively. The P-score cutoff was 55 and dot product of 0.65 for confidence. All hits were then manually confirmed by identifying 70% of c/y ions and 30% for a-B/y ions.
File: Aaegypti-maturetRNA-2.fasta
Description: All mature tRNA sequences in Ae. aegypti.
File: queA_larva_2.mzML
Description: Oligonucleotide LC-MS/MS file for mapping tRNA modifications of queA larvae, replicate 2. Data analysis was performed using RNAModMapper (RAMM) to the mature tRNA sequences with specific interest in the anticodon region of predicted Q-containing tRNAs (Asp-GTC, Tyr-GTA, His-GTG, and Asn-GTT). Monoisotopic mass was selected using 0.6 and 0.1 as the percursor ion tolerance and product ion tolerance, respectively. The P-score cutoff was 55 and dot product of 0.65 for confidence. All hits were then manually confirmed by identifying 70% of c/y ions and 30% for a-B/y ions.
Figure_S6.zip
File: FigureS6-downregulated-queA.csv
Description: Gene ontology classifications for downregulated transcripts in RNAseq of queA larvae.
Variables
- source: GO classification.
- term_name: Type of protein by function.
- term_id: GO classification ID.
- highlighted: GO output.
- adjusted_p_value: Sorted by adjusted pvalue.
- negative_log10_of_adjusted_p_value: Value of negative log 10 of adjusted pvalue.
- term_size: Total number of genes in term group.
- query_size: Genes sorted into group.
- intersection_size: Shared/overlapping genes.
- effective_domain_size: Total number of GO terms in database.
- intersections: List of genes in the GO classifciations.
Figure_S8.zip
File: FigureS8_expressionvalues_tyrosinepathway.csv
Description: Expression values from RNAseq data for genes involved in tyrosine metabolism pathway.
Variables
- Gene: Gene label in Ae. aegypti.
- Gene name: Long name of protein/function.
- Enzyme: Short name for protein.
- queA_3: Expression values for queA replicate 3.
- queA_2: Expression values for queA replicate 2.
- queA_1: Expression values for queA replicate 1.
- K12_1: Expression values for K12 replicate 1.
- K12_2: Expression values for K12 replicate 2.
- K12_3: Expression values for K12 replicate 3.
- queA_50_1: Expression values for queA + q replicate 1.
- queA_50_2: Expression values for queA + q replicate 2.
- queA_50_3: Expression values for queA + q replicate 3.
Table_S2.zip
File: TableS2-tyrosinepathway-CUB.csv
Description: The codon usage bias calculated using RSCU for transcripts involved in tyrosine pathway and ribosomal proteins for comparison.
Variables
- RSCU: Relative synonymous codon usage calculated for each codon.
- Codon: List of codons.
- GTPCH: RSCU values for codons of GTP cyclohydrolase.
- PTPS: RSCU values for codons of pyruvoyl tetrahydrobiopterin synthase.
- PAH: RSCU values for codons of phenylalanine hydroxylase.
- TH: RSCU values for codons of tyrosine hydroxylase.
- DopaDC: RSCU values for codons of aromatic L amino acid decarboxylase.
- DCE: RSCU values for codons of dopachrome conversion enzyme.
- PO: RSCU values for codons of tyrosinase phenoloxidase.
- ribo1: RSCU values for codons of ribosomal protein 1.
- ribo2: RSCU values for codons of ribosomal protein 2.
- ribo3: RSCU values for codons of ribosomal protein 3.
- ribo4: RSCU values for codons of ribosomal protein 4.
- ribo5: RSCU values for codons of ribosomal protein 5.
- ribo6: RSCU values for codons of ribosomal protein 6.
- ribo7: RSCU values for codons of ribosomal protein 7.
Figure_S9.zip
File: aedes_codon_usage.R
Description: R code for calculating the codon frequency for differentially expressed transcripts in RNAseq data.
File: all_amino_acids_freq.pdf
Description: Codon frequency of amino acids (Asn, His, Asp, Tyr) that may require Q-tRNA for decoding.
File: asn_freq.pdf
Description: Codon frequency of asparagine codons AAC and AAT in differentially expressed transcripts.
File: his_freq.pdf
Description: Codon frequency of histidine codons CAC and CAT in differentially expressed transcripts.
File: asp_freq.pdf
Description: Codon frequency of asparagine codons GAT and GAC in differentially expressed transcripts.
File: tyr_freq.pdf
Description: Codon frequency of tyrosine codons TAC and TAT in differentially expressed transcripts.
File: CUB-R-figure_1.csv
Description: Codon frequency of Q-decoded transcripts which were differentially expressed in RNAseq data.
Variables
- tyr_tat_up: Codon frequencies of TAT in upregulated transcripts in queA larvae.
- tyr_tac_up: Codon frequencies of TAC in upregulated transcripts in queA larvae.
- tyr_tat_down: Codon frequencies of TAT in downregulated transcripts in queA larvae.
- tyr_tac_down: Codon frequencies of TAC in downregulated transcripts in queA larvae.
- his_cat_up: Codon frequencies of CAT in upregulated transcripts in queA larvae.
- his_cac_up: Codon frequencies of CAC in upregulated transcripts in queA larvae.
- his_cat_down: Codon frequencies of CAT in downregulated transcripts in queA larvae.
- his_cac_down: Codon frequencies of CAC in downregulated transcripts in queA larvae.
- asn_aat_up: Codon frequencies of AAT in upregulated transcripts in queA larvae.
- asn_aac_up: Codon frequencies of AAC in upregulated transcripts in queA larvae.
- asn_aat_down: Codon frequencies of AAT in downregulated transcripts in queA larvae.
- asn_aac_down: Codon frequencies of AAC in downregulated transcripts in queA larvae.
- asp_gat_up: Codon frequencies of GAT in upregulated transcripts in queA larvae.
- asp_gac_up: Codon frequencies of GAC in upregulated transcripts in queA larvae.
- asp_gat_down: Codon frequencies of GAT in downregulated transcripts in queA larvae.
- asp_gac_down: Codon frequencies of GAC in downregulated transcripts in queA larvae.
Figure_S10.zip
File: FigureS10-tyrosine-grayvalue.csv
Description: Gray value of larvae for measuring coloration differences in queA, K12, and queA + tyrosine supplementation.
Variables
- Body location: Locations of the measurements.
- K12-1: K12 larva image 1.
- K12-2: K12 larva image 2.
- K12-3: K12 larva image 3.
- K12-4: K12 larva image 4.
- K12-5: K12 larva image 5.
- K12-6: K12 larva image 6.
- K12-7: K12 larva image 7.
- K12-8: K12 larva image 8.
- K12-9: K12 larva image 9.
- K12-10: K12 larva image 10.
- K12-11: K12 larva image 11.
- K12-12: K12 larva image 12.
- K12-13: K12 larva image 13.
- K12-14: K12 larva image 14.
- K12-15: K12 larva image 15.
- queA-1: queA larva image 1.
- queA-2: queA larva image 2.
- queA-3: queA larva image 3.
- queA-4: queA larva image 4.
- queA-5: queA larva image 5.
- queA-6: queA larva image 6.
- queA-7: queA larva image 7.
- queA-8: queA larva image 8.
- queA-9: queA larva image 9.
- queA-10: queA larva image 10.
- queA-11: queA larva image 11.
- queA-12: queA larva image 12.
- queA-13: queA larva image 13.
- queA-14: queA larva image 14.
- queA-15: queA larva image 15.
- queA + tyr -1: queA + tyrosine image 1.
- queA + tyr -2: queA + tyrosine image 2.
- queA + tyr -3: queA + tyrosine image 3.
- queA + tyr -4: queA + tyrosine image 4.
- queA + tyr -5: queA + tyrosine image 5.
- queA + tyr -6: queA + tyrosine image 6.
- queA + tyr -7: queA + tyrosine image 7.
- queA + tyr -8: queA + tyrosine image 8.
- queA + tyr -9: queA + tyrosine image 9.
- queA + tyr -10: queA + tyrosine image 10.
- queA + tyr -11: queA + tyrosine image 11.
- queA + tyr -12: queA + tyrosine image 12.
- queA + tyr -13: queA + tyrosine image 13.
- queA + tyr -14: queA + tyrosine image 14.
- queA + tyr -15: queA + tyrosine image 15.
Fig._S11.zip
File: baselineactivity_data_analysis_v1.csv
Description: analysis of baseline activity for mosquito larvae grown with different levels of Q
Variables
- code : Unique predation event identifier.
- event.no: Sequential predation event number within a video.
- event.time.st: Start time of scored baseline behavioral event.
- event.time.end : End time of scored baseline behavioral event.
- larvae.no: Larva number within the scored event or video.
- activity.type: Activity category: browsing, filtering, resting, or wriggling.
- time : Time spent in the scored activity category.
- video.label: Unique source video identifier.
- tment : Larval treatment group: K12, queA, or * queA+q.
- track.dur: Total scored tracking duration for the activity observation.
- video.id: Video replicate or recording identifier.
- video.time.skip: Video time interval skipped before scoring, such as 5min.
File: baselinedata_data_analysis_v1.csv
Description: baseline data used for baseline analysis of mosquito larvae movements
Variables
- code : Unique larval/track identifier.
- event.no: Sequential scored event number.
- event.time.st: Start time of scored baseline activity interval.
- event.time.end : End time of scored baseline activity interval.
- start.pos: Starting spatial position of the larva for the scored baseline event.
- w.burst : Number of discrete wriggle burst events.
- w.count : Total wriggling counts. Header has trailing space.
- video.label: Unique video identifier used to define replicate videos and clustering.
- tment : Larval treatment group: K12, queA, or * queA+q.
- track.dur : Duration of behavioral tracking interval, used as model offset.
- video.id : Video replicate or recording identifier.
- video.time.skip : Video time interval skipped before scoring, such as 2min.
Fig._S12.zip
Fig._S13.zip
File: point.data.csv
Description: Imported row index from original data export.
Variables
- Video: label for videos
- code: Unique predation event identifier.
- event.no: Sequential predation event number.
- event.time: Time stamp of event.
- frame.notice: Video frame number when predator noticed/detected prey.
- frame.strike: Video frame number at predator strike.
- pred.notice.time: Time when predator noticed/detected prey.
- pred.strike.st: Time of predator strike.
- lar.location.str: Prey/larval spatial grid location before strike.
- pred.suc: Predation outcome; Y = successful capture, N = unsuccessful capture/escape.
- strike.seq: Strike sequence category, if present.
- w.burst: Wriggle burst count
- w.count: Total wriggle count
- str.time.diff: Strike-associated timing difference
- notice.pred.pos: Predator position at notice
- notice.prey.pos: Prey position at notice
- handle.time: Handling time point
- handle.dur: Handling duration
- video.label: Unique source video identifier
- tment: Larval treatment group: K12, queA, or * queA+q.
- t.diff: Elapsed time from prey detection to predator strike, in seconds.
- Di.L: Larval/prey movement distance or displacement metric used to compute velocity
- Di.P: Predator movement distance or displacement metric used to compute velocity
- Di.LP: Distance between larva/prey and predator at prey detection/approach.
- Di.LP.norm: Normalized predator-prey distance.
- vel.L: Larval/prey velocity estimate.
- vel.P: Predator velocity estimate.
- frame.diff: Difference between notice frame and strike frame.
File: prey.pos_queA_q.csv
Description: Grid row label for arena position matrix (predator, queA + q).
Variables
- Replicate: Column labels of matrix (A-L)
- 1: Counts/frequencies of predator occurrence at arena grid cell 1 for queA + q.
- 2: Counts/frequencies of predator occurrence at arena grid cell 2 for queA + q.
- 3: Counts/frequencies of predator occurrence at arena grid cell 3 for queA + q.
- 4: Counts/frequencies of predator occurrence at arena grid cell 4 for queA + q.
- 5: Counts/frequencies of predator occurrence at arena grid cell 5 for queA + q.
- 6: Counts/frequencies of predator occurrence at arena grid cell 6 for queA + q.
- 7: Counts/frequencies of predator occurrence at arena grid cell 7 for queA + q.
- 8: Counts/frequencies of predator occurrence at arena grid cell 8 for queA + q.
- 9: Counts/frequencies of predator occurrence at arena grid cell 9 for queA + q.
- 10: Counts/frequencies of predator occurrence at arena grid cell 10 for queA + q.
- 11: Counts/frequencies of predator occurrence at arena grid cell 11 for queA + q.
- 12: Counts/frequencies of predator occurrence at arena grid cell 12 for queA + q.
- 13: Counts/frequencies of predator occurrence at arena grid cell 13 for queA + q.
- 14: Counts/frequencies of predator occurrence at arena grid cell 14 for queA + q.
- 15: Counts/frequencies of predator occurrence at arena grid cell 15 for queA + q.
- 16: Counts/frequencies of predator occurrence at arena grid cell 16 for queA + q.
- 17: Counts/frequencies of predator occurrence at arena grid cell 17 for queA + q.
- 18: Counts/frequencies of predator occurrence at arena grid cell 18 for queA + q.
- 19: Counts/frequencies of predator occurrence at arena grid cell 19 for queA + q.
- 20: Counts/frequencies of predator occurrence at arena grid cell 20 for queA + q.
- 21: Counts/frequencies of predator occurrence at arena grid cell 21 for queA + q.
File: prey.pos_queA.csv
Description: Grid row label for arena position matrix (predator, queA).
Variables
- Replicate: Column labels of matrix (A-L)
- 1: Counts/frequencies of predator occurrence at arena grid cell 1 for queA.
- 2: Counts/frequencies of predator occurrence at arena grid cell 2 for queA.
- 3: Counts/frequencies of predator occurrence at arena grid cell 3 for queA.
- 4: Counts/frequencies of predator occurrence at arena grid cell 4 for queA.
- 5: Counts/frequencies of predator occurrence at arena grid cell 5 for queA.
- 6: Counts/frequencies of predator occurrence at arena grid cell 6 for queA.
- 7: Counts/frequencies of predator occurrence at arena grid cell 7 for queA.
- 8: Counts/frequencies of predator occurrence at arena grid cell 8 for queA.
- 9: Counts/frequencies of predator occurrence at arena grid cell 9 for queA.
- 10: Counts/frequencies of predator occurrence at arena grid cell 10 for queA.
- 11: Counts/frequencies of predator occurrence at arena grid cell 11 for queA.
- 12: Counts/frequencies of predator occurrence at arena grid cell 12 for queA.
- 13: Counts/frequencies of predator occurrence at arena grid cell 13 for queA.
- 14: Counts/frequencies of predator occurrence at arena grid cell 14 for queA.
- 15: Counts/frequencies of predator occurrence at arena grid cell 15 for queA.
- 16: Counts/frequencies of predator occurrence at arena grid cell 16 for queA.
- 17: Counts/frequencies of predator occurrence at arena grid cell 17 for queA.
- 18: Counts/frequencies of predator occurrence at arena grid cell 18 for queA.
- 19: Counts/frequencies of predator occurrence at arena grid cell 19 for queA.
- 20: Counts/frequencies of predator occurrence at arena grid cell 20 for queA.
- 21: Counts/frequencies of predator occurrence at arena grid cell 21 for queA.
File: prey.pos_K12.csv
Description: Grid row label for arena position matrix (predator, K12).
Variables
- Replicate: Column labels of matrix (A-L)
- 1: Counts/frequencies of predator occurrence at arena grid cell 1 for K12.
- 2: Counts/frequencies of predator occurrence at arena grid cell 2 for K12.
- 3: Counts/frequencies of predator occurrence at arena grid cell 3 for K12.
- 4: Counts/frequencies of predator occurrence at arena grid cell 4 for K12.
- 5: Counts/frequencies of predator occurrence at arena grid cell 5 for K12.
- 6: Counts/frequencies of predator occurrence at arena grid cell 6 for K12.
- 7: Counts/frequencies of predator occurrence at arena grid cell 7 for K12.
- 8: Counts/frequencies of predator occurrence at arena grid cell 8 for K12.
- 9: Counts/frequencies of predator occurrence at arena grid cell 9 for K12.
- 10: Counts/frequencies of predator occurrence at arena grid cell 10 for K12.
- 11: Counts/frequencies of predator occurrence at arena grid cell 11 for K12.
- 12: Counts/frequencies of predator occurrence at arena grid cell 12 for K12.
- 13: Counts/frequencies of predator occurrence at arena grid cell 13 for K12.
- 14: Counts/frequencies of predator occurrence at arena grid cell 14 for K12.
- 15: Counts/frequencies of predator occurrence at arena grid cell 15 for K12.
- 16: Counts/frequencies of predator occurrence at arena grid cell 16 for K12.
- 17: Counts/frequencies of predator occurrence at arena grid cell 17 for K12.
- 18: Counts/frequencies of predator occurrence at arena grid cell 18 for K12.
- 19: Counts/frequencies of predator occurrence at arena grid cell 19 for K12.
- 20: Counts/frequencies of predator occurrence at arena grid cell 20 for K12.
- 21: Counts/frequencies of predator occurrence at arena grid cell 21 for K12.
Table_S3.zip
File: TableS3_SRM_Table_mosquitoes_Qpaper.csv
Description: Single reaction monitoring table for measuring mass transitions in LC-MS/MS.
This lists instrument parameters, precursor ions, and product ions for monitoring the detection of specific modifications. The LC-MS/MS data was then processed using Xcalibur 3.0 as described previously.
Variables
- Compound: Modification to be detected.
- Retention Time (min): Predicted retention time (RT) of compound.
- RT window: Time for flexible RT detection.
- Polarity: Mode of MS detection.
- Precursor: m/z value for precursor compound ion.
- Product (m/z): Mass of the predicted product ion.
- Collision energy (V): Energy required to ionize compound.
- RF Lens (V): Radio frequency lens values.