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Interactions between entomopathogenic fungi and nematodes in controlling larval and adult stages of the agave weevil, Scyphophorus acupunctatus

Guzmán-Franco, Ariel Wilbert1; Melitón-Rosalio, Julia1

Published Mar 18, 2026 on Dryad. https://doi.org/10.5061/dryad.34tmpg4zz

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Abstract

This dataset contains raw mortality data from laboratory bioassays evaluating the susceptibility of different developmental stages of Scyphophorus acupunctatus to entomopathogenic fungi and nematodes, including both single and dual inoculation treatments. We assessed the pathogenicity of isolates of the entomopathogenic fungi Beauveria bassiana, B. pseudobassiana, Metarhizium anisopliae, and M. pingshaense, as well as the entomopathogenic nematodes Steinernema feltiae, S. carpocapsae, and Heterorhabditis bacteriophora, against adult and larval stages of S. acupunctatus. Fungal isolates caused low mortality in adults (<20%) but induced moderate mortality in larvae (50–80%), with no significant differences among isolates. Nematode-induced mortality was also low in adults (<10%) but significantly higher in larvae, particularly with S. carpocapsae (~60%) compared with the other species (<10%). In dual-inoculation experiments using isolates C18-11 (B. bassiana) and L21-1 (M. anisopliae) in combination with S. carpocapsae, additive effects were observed in adults, with higher mortality (up to 50%) than in single treatments. In larvae, S. carpocapsae alone caused high mortality (~98%), similar to that observed in dual treatments, suggesting limited additional benefit from combined applications.