Dissimilar climatic niche is predictive of contrasting historical demographic changes and altitudinal shifts in related oak species (Quercus)
Data files
Nov 05, 2025 version files 47.42 KB
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README.md
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Three_species_QuercusQ_spp_9SSR.xlsx
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Abstract
The database contains information on nine microsatellite markers from three Mexican oak species. These data were used to infer microevolutionary patterns within the species and to explore their association with climatic affinities. The database includes the size of each locus for all dinucleotide microsatellites analyzed. This information provides valuable insights into the genetic diversity and structure of Mexican oaks, contributing to a better understanding of their adaptation to different environmental conditions.
Dataset DOI: 10.5061/dryad.37pvmcvxp
Description of the data and file structure
The dataset consists of a Three_species_QuercusQ_spp_9SSR.xlsx. This table shows genetic data from multiple populations (Pop1–Pop43), where each sample has been genotyped at a series of microsatellite loci (genetic markers). Each locus has two columns (ending in “a” and without “a”) — representing the two alleles an individual carries at that locus.
Nine nuclear simple sequence repeat (SSRs) loci (quru-GA-0C11, quru-GA-0M05, quru-GA-0C19, quru-GA-2F05, quru-GA-0M07, quru-GA-1C08, QpZAG36, QrZAG39, y QpZAG110), developed for Q. rubra, Q. petraea, and Q. robur (Steinkellner et al., 1997; Kampfer et al., 1998; Aldrich et al., 2003), were amplified, using fluorescently labelled (VIC, FAM, PET) forward primers. Reactions were performed using the Platinum Multiplex PCR Master Mix (Thermo Fisher Scientific) in a final volume of 6 µL. The temperatures of reactions were as follows: denaturalization step at 95 °C for 5 Min; 40 cycles of 95 °C for 30 seg, alignment temperature between 45 to 61 °C for 45 seg and 72 °C for 1 min; followed by a final step of extension at 72 °C for 10 min. PCR products were analysed in an ABI-PRISM 3300 Avant sequencer. Fragment sizes were determined using the program GeneMarker 2.6.4 (SoftGenetics) using Gene-scan-600 Liz as a size standard.
Access information
Other publicly accessible locations of the data:
- None
Data was derived from the following sources:
- None