Summary of Experimental Efforts

This dataset supports a preclinical study investigating the cardioprotective potential of Melissa officinalis ethanolic extract (MOE) against doxorubicin-induced cardiotoxicity (DIC) in female Wistar albino rats. The study included 21 rats divided into three groups: healthy control (CTRL), doxorubicin-treated (DOX), and doxorubicin plus MOE (DOX-MO). MOE was administered orally for 10 days, and doxorubicin was injected intraperitoneally on Day 8. The experiment evaluated in vivo cardiac function using echocardiography, ex vivo function using the Langendorff heart model, oxidative stress biomarkers in cardiac effluent, histological assessments, and antioxidant capacity of MOE via in vitro assays.

File Structure and Contents

Antioxidant_activity_MOE.xlsx

• Content: Total phenolic content (TPC) of MOE extract.
• Variables:
  • Values: Sample ID
  • mg GAE: Total phenolics (mg gallic acid equivalents/g dry extract)
  • AA: Ascorbic acid (µg/ml)
  • BHA: Butylated hydroxyanisole (µg/ml)
  • TROLOX: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (µg/ml)
• Assay: Folin-Ciocalteu method using microplate spectrophotometry.

Cardiodynamics.xlsx

• Content: Ex vivo heart function parameters recorded during Langendorff perfusion.
• Variables:
  • HR: Heart rate (beats/min)
  • dp/dt max and dp/dt min: Max/min pressure change (mmHg/s)
  • SLVP: Systolic left ventricular pressure (mmHg)
  • DLVP: Diastolic pressure (mmHg)
  • CF: Coronary flow (mL/min)
• Measurement Timepoints: Stabilization, post-ischemia (every 5 minutes for 40 minutes).

Colagen.xlsx

• Content: Picrosirius Red quantification of myocardial collagen deposition.
• Variables:
  • Collagen %: Area percentage of red-stained collagen fibers.
• Interpretation: Reflects myocardial fibrosis severity.

EHO.xlsx

• Content: Echocardiographic parameters measured via M-mode.
• Variables:
  • LVIDd, LVIDs: Left ventricular internal diameters (cm)
  • IVSd, IVSs: Septal wall thickness (cm)
  • PWd, PWs: Posterior wall thickness (cm)
  • EF: Ejection fraction (%)
  • FS: Fractional shortening (%)
  • LVEDV, LVESV: Volumes from Teichholz formula (mL)

HE_Histology.xlsx

• Content: Morphometric analysis of histological slides (H&E).
• Variables:
  • CSA: Cross-sectional area of cardiomyocytes (µm²)
  • LSD: Longitudinal section diameter (µm)
• Qualitative: Scores for inflammation, fiber waviness, and nuclear hypertrophy.

Oxidative_stress_efluent.xlsx

• Content: Markers of oxidative stress in coronary venous effluent.
• Variables:
  • O2−: Superoxide anion radical (absorbance 530 nm)
  • H2O2: Hydrogen peroxide (absorbance 610 nm)
  • NO2−: Nitrite (μmol/L via Griess assay)
  • TBARS: Lipid peroxidation index (nmol/mg protein)
• Timepoints: Stabilization and every 5–10 min during reperfusion.

Weight.xlsx

• Content: Animal body weights tracked throughout experiment.
• Timepoints: Day 0 (baseline), Day 7 (pre-doxorubicin), Day 10 (sacrifice day)
• Units: Grams (g)
• Variables:
  • CTRL I: Control group, Day 0 (g)
  • DOX I: DOX group, Day 0 (g)
  • DOX-MO I: DOX-MO group, Day 0 (g)
  • CTRL II: Control group, Day 7 (g)
  • DOX II: DOX group, Day 7 (g)
  • DOX-MO II: DOX-MO group, Day 7 (g)
  • CTRL III: Control group, Day 10 (g)
  • DOX III: DOX group, Day 10 (g)
  • DOX-MO III: DOX-MO group, Day 10 (g)

Abbreviations and Definitions

Missing Data

• No placeholder values used.
• Missing data are indicated by empty cells.

Related Resources

• Manuscript: Protective Effects of Melissa officinalis Ethanolic Extract on Doxorubicin-Induced Cardiotoxicity in a Rat Model
• Corresponding author: Dr. Isidora Milosavljević
  Email: isidora.milosavljevic@fmn.kg.ac.rs

License and Reuse

• License: Creative Commons CC0 1.0 Universal Public Domain Dedication applies to all materials deposited in this Dryad data package. These materials may be used, shared, modified, and distributed without restriction.
• Scope: This CC0 waiver applies only to the contents of this Dryad repository submission and does not affect the copyright status of the journal manuscript or any third-party materials included under separate terms.
• Citation: While not required by the CC0 license, citation of the associated manuscript is appreciated and strongly encouraged when reusing these data.
• Recommended Citation: Terzić J. et al. Protective Effects of Melissa officinalis Ethanolic Extract on Doxorubicin-Induced Cardiotoxicity in a Rat Model. [Journal], [Year]. DOI: [Insert when available]

Notes for Reuse and Replication

• Data derived directly from controlled laboratory experiments.
• All experimental design, methods, and statistical tests are described in detail in the associated manuscript.
• Sample sizes for each group: n = 7
• Measured values are representative of both functional and biochemical effects of treatment.
• This dataset is suitable for meta-analysis, replication, and educational use in cardiovascular pharmacology and toxicology research.