Data from: Seed pelleting and storage effects on germination of Pennycress (Thlaspi arvense L.)
Data files
Jul 17, 2025 version files 58.72 KB
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Pellet_storage.csv
56.14 KB
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README.md
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Abstract
Pennycress (Thlaspi arvense L.) is an emerging bioenergy oilseed crop of interest to farmers in the Upper Midwestern United States. Improved lines with beneficial agronomic traits are being developed, including lines with reduced silicle shattering and altered seed coat characteristics, though planting and establishment is still challenging for this small‐seeded crop. A controlled‐environment experiment was conducted to assess the impact of seed treatment and pelleting on pennycress germination and seedling vigor of four pennycress lines (three black and one golden colored seed) following storage for 0, 1, 3, 9, and 12 months in warm or cold conditions (23°C or 10°C, respectively). Seeds were either treated with a gibberellin A4+7 (GA) soak (positive control), seed pelleting, or pelleting with GA added to the binder solution in addition to the untreated control (negative control). After each storage duration, seeds were germinated in darkness and counted daily for 7 days to obtain total germination. Vigor indices were calculated using changes in daily germination values. For black‐seeded lines, GA treatment increased germination over the untreated by 5%–75% and pelleting alone increased germination by 4%–30%, but only until 3 months of storage. The GA treatment did not increase germination of the golden‐seeded line, and pelleting decreased germination after 3 months of storage. The GA soak treatment was most effective at improving seed vigor indices compared with other treatments, while pelleting negatively affected vigor scores at 9 and 12 months of storage. Similar effects on germination from treatment were observed under both warm and cold storage conditions. Results indicate seed treatment can benefit germination and vigor within 3 months of storage for black‐seeded lines but may negatively affect performance of golden‐seeded lines, or all pennycress seed types after 9 months of storage.
Dataset DOI: 10.5061/dryad.41ns1rnss
Description of the data and file structure
This dataset contains information related to tested pennycress lines (4 in total) and the four seed treatments evaluated (untreated, a gibberellic acid soaking treatment, a pelleting treatment, and a pelleted treatment using gibberellic acid in the binder solution). Variables measured include storage environment temperature (23C or 10 °C) and duration of storage (0, 1, 3, 9, or 12 months). Collected data on each treatment includes cumulative germination over a seven-day period after the duration of storage was achieved. Data related to seed weight and diameter for each treatment is also included for the 0 month duration to provide baseline data related to seed characteristics. Missing data for a given variable within a plot is denoted solely with a “.” value as data was not or could not be collected in the specific instance.
Files and variables
File: Pellet_storage.csv
Variables
- Row 1 - Column Name: Column contains the descriptive titles for the first six rows of the worksheet, where each row describes a component of the data included within the spreadsheet.
- Variety: One of the four varieties evaluated is named within this column.
- Seed treatment: One of the four applied seed treatments is named in this column.
- Block (Rep): This relates to when this experiment was performed in time.
- Plate: Refers to individual units sampled at each timepoint within each block.
- Storage Duration: This value in months is the time after treatment was applied when seeds were tested for germination.
- Storage Temperature: Refers to temperature of the storage environment. Does not apply to the initial seedsets (0 months) because no storage had occurred at that time.
- Total Germination day 3: Cumulative germination percentage on day 3
- Total Germination Day 4: Cumulative germination percentage on day 4
- Total Germination Day 5: Cumulative germination percentage on day 5
- Total Germination Day 6: Cumulative germination percentage on day 6
- Total Germination Day 7: Cumulative germination percentage on day 7
- Seed weight: Weight of 1000 seeds for each treatment
- Seed Size: Weighted average diameter of seeds of each treatment in mm
- Weight Difference: Change in weight of 5g of pelleted seeds in grams after shaking
Access information
Other publicly accessible locations of the data:
- GEMS Informatics platform