Data from: Biochemical and molecular dynamics of metabolic flux and detoxification responses in phosphine-resistant Tribolium castaneum
Data files
Mar 26, 2026 version files 36.63 KB
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All_R_Script.R
2.99 KB
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BHM.csv
263 B
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Bioassay_data.csv
608 B
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DEC.csv
373 B
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DEP.csv
439 B
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Enzyme_data.csv
7.19 KB
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Gene_expression_data.csv
18.56 KB
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MEC.csv
184 B
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MEP.csv
250 B
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README.md
5.77 KB
Abstract
Phosphine (PH₃) is widely used for stored-product pest management, yet increasing resistance in Tribolium castaneum populations poses a significant challenge. This study investigates the metabolic modulation and detoxification response changes observed in phosphine-resistant T. castaneum. Bioassay data analyzed via a Bayesian Hierarchical Model (BHM) revealed substantial heterogeneity in LC₅₀ values, with resistance ratios ranging from 3.73-fold in Shillong to 92.96-fold in Patiala, highlighting pronounced population-specific heterogeneity. There was a significant decline in the activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from 9.20 ± 0.35 nmol/mg in the susceptible strain to 4.88 ± 0.08 nmol/mg in the highly resistant Patiala populations, whereas there was a two-fold rise in triose phosphate isomerase (TPI) activity in the resistant population. Detoxification enzymes, including carboxylesterase (CE) and glutathione S-transferases (GSTs), exhibited marked elevation, with CE activity rising to 7.82-fold and GST up to 7.65-fold. Although acetylcholinesterase (AChE) enzymatic activity remained unchanged. Quantitative gene expression analysis mirrored enzymatic trends. Gene expression profiling revealed pronounced transcriptional modulation. GAPDH expression was reduced up to 0.03-fold, while TPI transcripts increased by 9.10-fold, indicating re-programming of the lytic pathway. Detoxification-associated genes showed strong induction, with elevation of CE by 94.7-fold and GST Delta family genes by 5.6–26.9-fold and AChE activity by 56-fold, suggesting their role in xenobiotic metabolism. Correlation and principal component analyses confirmed strong concordance between enzymatic activities and transcriptional profiles. Overall, phosphine-resistant T. castaneum populations exhibit coordinated metabolic adjustments and enhanced detoxification responses, reflecting a complex, system-wide adaptive shift.
Dataset DOI: [10.5061/dryad.573n5tbpg] (https://doi.org/10.5061/dryad.573n5tbpg)
This contains the data used for experimental analysis for the article: Biochemical and molecular dynamics of metabolic flux and detoxification responses in phosphine-resistant Tribolium castaneum (Authors:M.G. Deeksha, Sabtharishi Subramanian , Niraj Guleria , Suresh M. Nebapure, Anil Dahuja, Sagar Doddachowdappa, Ramcharan Bhattacharya)
Description of the data and file structure
Files and variables
File: BHM.csv
Description: Dataset of Bayesian Hierarchical Modeling including LC₅₀ (lethal concentration 50) values, standard errors, and resistance ratios for phosphine-resistant populations of Tribolium castaneum compared with a laboratory-susceptible population
Variables
population location in the first column, LC₅₀ (lethal concentration 50) values in the second column, standard error in the third column, and resistance ratio in the fourth column
File: Bioassay_data.csv
Description: The dataset presents bioassay data for seven phosphine-resistant populations of Tribolium castaneum in comparison with a laboratory-susceptible strain
Variables
population in the first column; LC₅₀ (mg/L) in the second column; fiducial limits in the third column; slope ± standard error in the fourth column; chi-square value in the fifth column; heterogeneity in the sixth column; degrees of freedom in the seventh column; and resistance ratio in the eighth column
File: DEP.csv
Description: The dataset is of Detoxification enzymes and their gene expression arranged for principal component analysis (PCA), encompassing seven phosphine-resistant populations of Tribolium castaneum in comparison with a laboratory-susceptible strain
Variables
population data (first column); carboxyl esterase enzyme assay (CE EA) (second column); glutathione S-transferase enzyme assay (GST EA) (third column); acetylcholinesterase enzyme assay (AChE EA) (fourth column); carboxyl esterase gene expression (CE GE) (fifth column); glutathione S-transferase delta 1 gene expression (GSTD1 GE) (sixth column); glutathione S-transferase delta 2 gene expression (GSTD2 GE) (seventh column); glutathione S-transferase delta 3 gene expression (GSTD3 GE) (eigth column); and acetylcholinesterase gene expression (AChE GE) (ninth column)
File: MEP.csv
Description: The dataset is of Metabolic enzymes and their gene expression arranged for principal component analysis (PCA), encompassing seven phosphine-resistant populations of Tribolium castaneum in comparison with a laboratory-susceptible strain
Variables
population data in the first column; GAPDH enzyme assay (GAPDH EA) in the second column; TPI enzyme assay (TPI EA) in the third column; GAPDH gene expression (GAPDH GE) in the fourth column; and TPI gene expression (TPI GE) in the fifth column.”
File: DEC.csv
Description: The dataset is of Detoxification enzymes and their gene expression, arranged for correlation analysis, encompassing seven phosphine-resistant populations of Tribolium castaneum in comparison with a laboratory-susceptible strain
Variables
population data (first column); carboxyl esterase enzyme assay (CE EA) (second column); glutathione S-transferase enzyme assay (GST EA) (third column); acetylcholinesterase enzyme assay (AChE EA) (fourth column); carboxyl esterase gene expression (CE GE) (fifth column); glutathione S-transferase delta 1 gene expression (GSTD1 GE) (sixth column); glutathione S-transferase delta 2 gene expression (GSTD2 GE) (seventh column); glutathione S-transferase delta 3 gene expression (GSTD3 GE) (eigth column); and acetylcholinesterase gene expression (AChE GE) (ninth column)
File: MEC.csv
Description: The dataset is of Metabolic enzymes and their gene expression, arranged for correlation analysis, encompassing seven phosphine-resistant populations of Tribolium castaneum in comparison with a laboratory-susceptible strain
Variables
population data in the first column; GAPDH enzyme assay (GAPDH EA) in the second column; TPI enzyme assay (TPI EA) in the third column; GAPDH gene expression (GAPDH GE) in the fourth column; and TPI gene expression (TPI GE) in the fifth column.”
File: Enzyme_data.csv
Description: The dataset comprises enzyme activity data for detoxification and metabolic enzymes, along with their one-way ANOVA analysis, encompassing seven phosphine-resistant populations of Tribolium castaneum in comparison with a laboratory-susceptible strain
Variables
one-way ANOVA–analyzed data for carboxyl esterase, glutathione S-transferase, acetylcholinesterase, glyceraldehyde-3-phosphate dehydrogenase, and triose phosphate isomerase
File: Gene_expression_data.csv
Description: The dataset comprises gene expression data for detoxification and metabolic enzymes, along with their one-way ANOVA analysis, encompassing seven phosphine-resistant populations of Tribolium castaneum in comparison with a laboratory-susceptible strain
Variables
one-way ANOVA–analyzed data for carboxyl esterase, glutathione S-transferase delta family genes, acetylcholinesterase, glyceraldehyde-3-phosphate dehydrogenase, and triose phosphate isomerase
File: All_R_Script.R
Description: R codes for Bayesian Hierarchical Model (BHM), Principal Component Analysis, and correlation analysis
The R script is designed to perform Bayesian Hierarchical Modeling (BHM), Principal Component Analysis (PCA), and correlation analysis for the Tribolium castaneum dataset