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Data from: Rapid formation of non-spatial hippocampal representations consistent with behavioral timescale synaptic plasticity is modulated by entorhinal input

Dorian, Conor1; Taxidis, Jiannis23; Arac, Ahmet1; Golshani, Peyman1

Published Apr 02, 2026 on Dryad. https://doi.org/10.5061/dryad.573n5tbpp

Data files

Apr 02, 2026 version files 49.51 GB

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Abstract

Behavioral timescale synaptic plasticity (BTSP) is a form of synaptic potentiation where a single plateau potential in hippocampal neurons forms a place field during spatial learning. However, it remains unknown whether BTSP also forms non-spatial responses and what roles the medial and lateral entorhinal cortex (MEC and LEC) play in driving non-spatial BTSP. Using two-photon calcium imaging of CA1 pyramidal neurons in mice learning an odor-cued working memory task, we observed spontaneously occurring large plateau-like calcium events during odor cues, forming stable odor representations. Using holographic optogenetics, we induced similar plateau-like calcium events in single neurons that were followed by novel odor representations. Chemogenetic inhibition of MEC reduced the frequency of plateau-like events, whereas LEC inhibition reduced their efficiency in forming odor representations. Together, our findings demonstrate that rare large somatic calcium events, consistent with BTSP, precede and drive novel odor representations in a manner differentially regulated by medial and lateral entorhinal cortex. This dataset contains all preprocessed data from the publication [Dorian et al 2026](Rapid formation of non-spatial hippocampal representations consistent with behavioral timescale synaptic plasticity is modulated by entorhinal input). All figure panels with comparisons and statistics can be generated with the data here and code found on GitHub, which has been written in MATLAB version 2021a.