Data from: Ameliorative effect of roxithromycin against prostate inflammation-induced chronic pelvic pain in a preclinical model
Data files
Feb 20, 2026 version files 10.68 KB
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BW_and_PI.csv
1.16 KB
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EBD.csv
314 B
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GSH.csv
364 B
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Histology_score.csv
140 B
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IR_SCORE.csv
685 B
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Pain_Score.csv
458 B
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PGE2.csv
443 B
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README.md
5.95 KB
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SOD.csv
317 B
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TBARS.csv
361 B
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Time_Course.csv
139 B
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TNF-α.csv
353 B
Abstract
In view of the well-known anti-inflammatory property of roxithromycin, the present study was carried out to evaluate its efficacy in ameliorating prostate inflammation-induced chronic pelvic pain in a rat model. Inflammation was induced by a single intra-prostatic injection of carrageenan, where a time course study revealed a peak histology score on day 14. The efficacy study was carried out in four sets of rats, each comprising 5 groups, which included the respective normal and experimental controls (NC, EC). The drugs (roxithromycin 5 and 20 mg/kg, diclofenac 5 mg/kg) were given daily orally, and pain score and body weight were evaluated at different time intervals. The prostatic index, vascular permeability, tissue levels of TNF-α and PGE2, thiobarbituric acid reactive substances, glutathione and superoxide dismutase, histology score, and immunoreactivity of COX-2 and iNOS were evaluated on day 14. Like diclofenac, roxithromycin decreased pain scores, and the rats gained weight during the study period as compared to the EC group. The beneficial effect of roxithromycin was also evident from the dose-dependent restoration of markers of inflammation and oxidative stress.This study shows that by alleviating pain and suppressing inflammation and oxidative stress,roxithromycin has a potential to provide relief in prostatitis.
We have submitted our raw data. Time_course study (Time_Course.csv), Body weight and prostatic index (BW_and_PI.csv), Evans blue dye extraction (EBD.csv), Oxidative marker GSH (GSH.csv), Histology score (Histology_score.csv), Immunoreactivity score (IR_SCORE.csv), Pain_score (Pain_Score.csv), Inflammatory marker PGE2 (PGE2.csv) Oxidative marker SOD (SOD.csv) Oxidative marker TBARS (TBARS.csv), Inflammatory marker TNF-α (TNF-α.csv)
Descriptions
Time_course study
* Day 0 - Histology score of rats sacrificed on day 0
* Day 7 - Histology score of rats sacrificed on day 7
* Day 14 - Histology score of rats sacrificed on day 14
* Day 21 - Histology score of rats sacrificed on day 21
* n = sample number used for the calculation
* Mean & SEM = Standard Error of mean
BW_and_PI
- NC: Normal control - No intervention is done
- EC: Experimental control - Intra- prostastic injection of carrageenan given
- R5: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg
- R20: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
- D5: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg
- n = sample number used for the calculation
- Body weight of rat calculated in grams and prostate weight of rat in milligrams
- Prostatic Index = prostate weight/Body weight
- Mean & SEM = Standard Error of mean
Pain score
- NC: Normal control - No intervention is done
- EC: Experimental control - Intra- prostastic injection of carrageenan given
- R5: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg
- R20: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
- D5: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg
- n = sample number used for the calculation
- Mean & SEM = Standard Error of mean
Evans blue dye extravasation
- NC: Normal control - No intervention is done
- EC: Experimental control - Intra- prostastic injection of carrageenan given
- R5: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg
- R20: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
- D5: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg
- n = sample number used for the calculation
- Mean & SEM = Standard Error of mean
- Absorbance of extracted Evans blue dye is expressed as ng/mg tissue
Oxidative marker GSH
- NC: Normal control - No intervention is done
- EC: Experimental control - Intra- prostastic injection of carrageenan given
- R5: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg
- R20: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
- D5: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg
- n = sample number used for the calculation
- Mean & SEM = Standard Error of mean
- Tissue levels of GSH is expressed as µg/g tissue
Oxidative marker SOD
- NC: Normal control - No intervention is done
- EC: Experimental control - Intra- prostastic injection of carrageenan given
- R5: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg
- R20: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
- D5: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg
- n = sample number used for the calculation
- Mean & SEM = Standard Error of mean
- Absorbance of SOD activity is expressed as U/mg protein
Oxidative marker TBARS
- NC: Normal control - No intervention is done
- EC: Experimental control - Intra- prostastic injection of carrageenan given
- R5: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg
- R20: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
- D5: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg
- n = sample number used for the calculation
- Mean & SEM = Standard Error of mean
- Tissue level of TBARS is expressed as nmol/g tissue
Inflammatory marker TNF-α
- NC: Normal control - No intervention is done
- EC: Experimental control - Intra- prostastic injection of carrageenan given
- R5: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg
- R20: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
- D5: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg
- n = sample number used for the calculation
- Mean & SEM = Standard Error of mean
- TNF-α levels were expressed as pg/100 mg tissue
Inflammatory marker PGE2
- NC: Normal control - No intervention is done
- EC: Experimental control - Intra- prostastic injection of carrageenan given
- R5: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg
- R20: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
- D5: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg
- n = sample number used for the calculation
- Mean & SEM = Standard Error of mean
- PGE2 levels were expressed as pg/100 mg tissue
Histology score
- NC: Normal control - No intervention is done
- EC: Experimental control - Intra- prostastic injection of carrageenan given
- R5: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg
- R20: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
- D5: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg
- n = sample number used for the calculation
- Mean & SEM = Standard Error of mean
Immunoreactivity score
- NC: Normal control - No intervention is done
- EC: Experimental control - Intra- prostastic injection of carrageenan given
- R5: Intra- prostastic injection of carrageenan + Roxithromycin 5 mg/kg
- R20: Intra- prostastic injection of carrageenan + Roxithromycin 20 mg/kg
- D5: Intra- prostastic injection of carrageenan + Diclofenac 5 mg/kg
- n = sample number used for the calculation
- Mean & SEM = Standard Error of mean